cDNA Clone KIAA1390

• Plasmid: pGEX6P1 KIAA1390 C137A
• Parent Plasmid: pGEX6P1
• Expressed: GST-KIAA1390 C137A
• Genbank: Q8N5J2
• Tag: GST

Sequence of Insert

agatctATGGAATACCATCAGCCTGAGGATCCAGCCCCTGGTAAGGCCGGGACTGCAGAAGCAGTCATCCCTGAAAACCATGAGGTTCTGGCAGGCCCAGATGAGCACCCTCAGGACACAGATGCAAGAGATGCTGATGGGGAGGCTAGAGAACGGGAGCCAGCAGACCAAGCTTTGCTGCCTAGCCAGTGTGGGGACAACCTTGAGTCCCCTCTGCCTGAAGCTAGCTCAGCTCCACCGGGGCCAACCCTTGGGACACTGCCTGAAGTAGAGACAATAAGGGCATGCTCCATGCCCCAGGAGCTTCCTCAGTCCCCCAGGACCCGACAGCCTGAGCCAGATTTCTACTGTGTCAAGTGGATCCCTTGGAAAGGAGAACAGACACCCATCATCACCCAGAGCACTAACGGCCCTGCCCCTCTCCTTGCCATCATGAACATCCTCTTTCTTCAGTGGAAGGTGAAGCTCCCCCCGCAGAAGGAAGTGATCACATCGGATGAGCTCATGGCCCATCTTGGAAACTGCCTCCTGTCCATCAAGCCCCAGGAGAAGTCAGAGGGACTTCAGCTTAATTTTCAGCAGAATGTGGATGATGCAATGACAGTGCTGCCTAAACTGGCCACAGGTCTGGATGTCAATGTGCGATTCACAGGCGTCTCTGATTTTGAGTATACACCCGAGTGCAGTGTCTTTGACCTGCTAGGCATACCTCTGTACCATGGCTGGCTTGTTGATCCACAGAGTCCTGAGGCTGTGCGTGCAGTTGGGAAACTGAGTTACAACCAGCTGGTGGAGAGGATCATCACCTGCAAACACTCCAGTGACACCAACCTCGTGACAGAAGGCCTGATTGCAGAGCAGTTCCTGGAGACCACCGCGGCCCAGCTGACCTACCACGGACTGTGTGAGCTGACAGCAGCTGCTAAGGAGGGTGAACTTAGCGTCTTTTTCCGAAACAACCACTTTAGCACCATGACTAAGCATAAGAGTCACTTATACCTACTGGTCACTGACCAGGGCTTTCTACAGGAGGAGCAAGTCGTATGGGAGAGCCTGCACAATGTGGATGGAGACAGCTGCTTTTGTGACTCTGACTTTCACCTGAGTCATTCCCTGGGCAAGGGGCCTGGAGCAGAAGGTGGGAGTGGCTCCCCAGAAAAGCAGCTGCAGGTAGACCAGGACTACCTGATTGCTCTGTCCCTGCAGCAGCAACAGCCACGAGGCCCGCTGGGGCTTACCGACTTGGAGCTGGCCCAGCAGCTTCAGCAAGAGGAGTATCAACAGCAGCAGGCAGCGCAGCCAGTGCGGATGCGGACGCGGGTCCTGTCACTGCAGGGGAGAGGAGCCACATCTGGACGCCCAGCCGGGGAGCGTCGGCAGAGGCCGAAGCACGAGTCAGACTGCATTCTGCTGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEYHQPEDPAPGKAGTAEAVIPENHEVLAGPDEHPQDTDARDADGEAREREPADQALLPSQCGDNLESPLPEASSAPPGPTLGTLPEVETIRACSMPQELPQSPRTRQPEPDFYCVKWIPWKGEQTPIITQSTNGPAPLLAIMNILFLQWKVKLPPQKEVITSDELMAHLGNCLLSIKPQEKSEGLQLNFQQNVDDAMTVLPKLATGLDVNVRFTGVSDFEYTPECSVFDLLGIPLYHGWLVDPQSPEAVRAVGKLSYNQLVERIITCKHSSDTNLVTEGLIAEQFLETTAAQLTYHGLCELTAAAKEGELSVFFRNNHFSTMTKHKSHLYLLVTDQGFLQEEQVVWESLHNVDGDSCFCDSDFHLSHSLGKGPGAEGGSGSPEKQLQVDQDYLIALSLQQQQPRGPLGLTDLELAQQLQQEEYQQQQAAQPVRMRTRVLSLQGRGATSGRPAGERRQRPKHESDCILL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU55337
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate Cys 137 to Ala in 49563