cDNA Clone NCK1

• Synonyms: nck-1, NCKalpha
• Plasmid: pcDNA5-FRT/TO C-mCherry DU-NCK1
• Parent Plasmid: pcDNA5-FRT/TO C-mCherry
• Expressed: NCK1-mCherry
• Genbank: NM_001291999.1
• Tag: mCherry
• Species: Human

Sequence of Insert

GGATCCATGGCAGAAGAAGTGGTGGTAGTAGCCAAATTTGATTATGTGGC
CCAACAAGAACAAGAGTTGGACATCAAGAAGAATGAGAGATTATGGCTTC
TGGATGATTCTAAGTCCTGGTGGCGAGTTCGAAATTCCATGAATAAAACA
GGTTTTGTGCCTTCTAACTATGTGGAAAGGAAAAACAGTGCTCGGAAAGC
ATCTATTGTGAAAAACCTAAAGGATACCTTAGGCATTGGAAAAGTGAAAA
GAAAACCTAGTGTGCCAGATTCTGCATCTCCTGCTGATGATAGTTTTGTT
GACCCAGGGGAACGTCTCTATGACCTCAACATGCCCGCTTATGTGAAATT
TAACTACATGGCTGAGAGAGAGGATGAATTATCATTGATAAAGGGGACAA
AGGTGATCGTCATGGAGAAATGCAGTGATGGGTGGTGGCGTGGTAGCTAC
AATGGACAAGTTGGATGGTTCCCTTCAAACTATGTAACTGAAGAAGGTGA
CAGTCCTTTGGGTGACCATGTGGGTTCTCTGTCAGAGAAATTAGCAGCAG
TCGTCAATAACCTAAATACTGGGCAAGTGTTGCATGTGGTACAGGCTCTT
TACCCATTCAGCTCATCTAATGATGAAGAACTTAATTTCGAGAAAGGAGA
TGTAATGGATGTTATTGAAAAACCTGAAAATGACCCAGAGTGGTGGAAAT
GCAGGAAGATCAATGGTATGGTTGGTCTAGTACCAAAAAACTATGTTACC
GTTATGCAGAATAATCCATTAACTTCAGGTTTGGAACCATCACCTCCACA
GTGTGATTACATTAGGCCTTCACTCACTGGAAAGTTTGCTGGCAATCCTT
GGTATTATGGCAAAGTCACCAGGCATCAAGCAGAAATGGCATTAAATGAA
AGAGGACATGAAGGGGATTTCCTCATTCGTGATAGTGAATCTTCGCCAAA
TGATTTCTCAGTATCACTAAAAGCACAAGGGAAAAACAAGCATTTTAAAG
TCCAACTAAAAGAGACTGTCTACTGCATTGGGCAGCGTAAATTCAGCACC
ATGGAAGAACTTGTAGAACATTACAAAAAGGCACCAATTTTTACAAGTGA
ACAAGGAGAAAAATTATATCTTGTCAAGCATTTATCAGCGGCCGC

Amino Acid Sequence

MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGF
VPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDP
GERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNG
QVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYP
FSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVM
QNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERG
HEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTME
ELVEHYKKAPIFTSEQGEKLYLVKHLSAAAMVSKGEEDNMAIIKEFMRFK
VHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQF
MYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQD
GEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRL
KLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYE
RAEGRHSTGGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28689
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 23848
• Low Temp: No