cDNA Clone NCK2

• Plasmid: pcDNA5-FRT/TO C-mCherry DU-NCK2
• Parent Plasmid: pcDNA5-FRT/TO C-mCherry DU
• Expressed: NCK2-mCherry
• Genbank: NM_003581.4 → NP_003572.2
• Tag: mCherry
• Species: Human

Sequence of Insert

ggatccATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGC
CCAGCAGGACCAGGAGCTGGACATCAAGAAGAACGAGCGGCTGTGGTTGC
TGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAGGACG
GGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGG
CTCCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCA
GGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAG
TACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCC
GGCCTTCGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCC
TGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCGACGGTTGG
TGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGT
CTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCC
TGCGCAAGGGCGCCTCGCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCAT
GTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACCGAGGAGGAGCTCAA
CTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACC
CCGAGTGGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCC
AAAAACTACGTGGTGGTCCTCAGTGACGGGCCTGCCCTGCACCCTGCGCA
CGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTCGCGG
GCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCC
CTCAACGAGCGGGGCGTGGAGGGCGACTTCCTCATTAGGGACAGCGAGTC
CTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAAC
ACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGC
TTCCACACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTT
CACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGCAGgcgg
ccgc

Amino Acid Sequence

MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGY
VPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYP
ANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWR
GSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVV
QTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKN
YVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALN
ERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFH
TMDELVEHYKKAPIFTSEHGEKLYLVRALQAAAMVSKGEEDNMAIIKEFM
RFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILS
PQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSS
LQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIK
QRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVE
QYERAEGRHSTGGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28690
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cytoplasmic protein NCK2 isoform A. Parental 23848
• Low Temp: No