cDNA Clone NEK1

• Synonyms: ALS24; NY-REN-55; SRPS2; SRPS2A; SRTD6
• Plasmid: pFastbacDual 6P1 NEK1 tv1 M1-I503
• Parent Plasmid: pFastbac 6P1
• Expressed: GST-NEK1 (M1 - I503)
• Genbank: NM_001199397.1
• Tag: GST

Sequence of Insert

GGATCCATGGAGAAGTATGTTAGACTACAGAAGATTGGAGAAGGTTCATTTGGAAAAGCCATTCTTGTTAAATCTACAGAAGATGGCAGACAGTATGTTATCAAGGAAATTAACATCTCAAGAATGTCCAGTAAAGAAAGAGAAGAATCAAGGAGAGAAGTTGCAGTATTGGCAAACATGAAGCATCCAAATATTGTCCAGTATAGAGAATCATTTGAAGAAAATGGCTCTCTCTACATAGTAATGGATTACTGTGAGGGAGGGGATCTGTTTAAGCGAATAAATGCTCAGAAAGGCGTTTTGTTTCAAGAGGATCAGATTTTGGACTGGTTTGTACAGATATGTTTGGCCCTGAAACATGTACATGATAGAAAAATTCTTCATCGAGACATTAAATCTCAGAACATATTTTTAACTAAAGATGGAACAGTACAACTTGGAGATTTTGGAATTGCTAGAGTTCTTAATAGTACTGTAGAGCTGGCTCGAACTTGCATAGGGACCCCATACTACTTGTCACCTGAAATCTGTGAAAACAAACCTTACAATAATAAAAGTGACATTTGGGCTCTGGGGTGTGTCCTTTATGAGCTGTGTACACTTAAACATGCTTTTGAAGCTGGCAGTATGAAAAACCTGGTACTGAAGATAATATCTGGATCTTTTCCACCTGTGTCTTTGCATTATTCCTATGATCTCCGCAGTTTGGTGTCTCAGTTATTTAAAAGAAATCCTAGGGATAGACCATCAGTCAACTCCATATTGGAGAAAGGTTTTATAGCCAAACGCATTGAAAAGTTTCTCTCTCCTCAGCTTATTGCAGAAGAATTTTGTCTAAAAACATTTTCGAAGTTTGGATCACAGCCTATACCAGCTAAAAGACCAGCTTCAGGACAAAACTCGATTTCTGTTATGCCTGCTCAGAAAATTACAAAGCCTGCCGCTAAATATGGAATACCTTTAGCATATAAGAAATATGGAGATAAAAAATTACACGAAAAGAAACCACTGCAAAAACATAAACAGGCCCATCAAACTCCAGAGAAGAGAGTGAATACTGGAGAAGAAAGGAGGAAAATATCTGAGGAAGCAGCAAGAAAGAGAAGGCTGGAATTTATTGAAAAAGAAAAGAAACAAAAGGATCAGATTATTAGTTTAATGAAGGCTGAACAAATGAAAAGGCAAGAAAAGGAAAGGTTGGAAAGAATAAATAGGGCCAGGGAACAAGGATGGAGAAATGTGCTAAGTGCTGGTGGAAGTGGTGAAGTAAAGGCTCCTTTTCTGGGCAGTGGAGGGACTATAGCTCCATCATCTTTTTCTTCTCGAGGACAGTATGAACATTACCATGCCATTTTTGACCAAATGCAGCAACAAAGAGCAGAAGATAATGAAGCTAAATGGAAAAGAGAAATATATGGTCGAGGTCTTCCAGAAAGAGGAATTCTGCCTGGAGTTCGTCCAGGATTTCCTTATGGGGCTGCAGGTCATCACCATTTTCCTGATGCTGATGATATTTAAAGAAAAACTTTGAAAAGATTGAAGGCGGTGTCTAAACAAGCCAATGCAAACAGGCAAAAAGGGCAGCTAGCTGTAGAAAGAGCTAAACAAGTAGAAGAGTTCCTGCAGCGAAAACGGGAAGCTATGCAGAATAAAGCTCGAGCCGAAGGACATATGGGAATCCTGCAAAACCTGGCAGCTATGTATGGAGGCAGGCCCAGCTCTTCAAGAGGAGGGAAGCCAAGAAACAAAGAGGAAGAGGTTTATCTGGCAAGACTGAGGCAAATAAGACTACAGAATTTCAATGAGCGCCAACAGATTAAAGCCAAACTTCGTGGTGAAAAGAAAGAAGCTAATCATTCTGAAGGACAAGAAGGAAGTGAAGAGGCTGACATGAGGCGCAAAAAAATCGAATCACTGAAGGCCCATGCAAATGCACGTGCTGCTGTACTAAAAGAACAACTAGAACGAAAGAGAAAGGAGGCTTATGAGAGAGAAAAAAAAGTGTGGGAAGAGCATTTGGTGGCTAAAGGAGTTAAGAGTTCTGATGTTTCTCCACCTTTGGGACAGCATGAAACAGGTGGCTCTCCATCAAAGCAACAGATGAGATCTGTTATTTCTGTAACTTCAGCTTTGAAAGAAGTTGGCGTGGACAGTAGTTTAACTGATACCCGGGAAACTTCAGAAGAGATGCAAAAGACCAACAATGCTATTTCAAGTAAGCGAGAAATACTTCGTAGATTAAATGAAAATCTTAAAGCTCAAGAAGATGAAAAAGGAAAGCAGAATCTCTCTGATACTTTTGAGATAAATGTTCATGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTGATCGCAAGAAGTGGGAGGCAGGAGGTCAACTTGTGATTCCTCTGGATGAGTTAACACTAGATACATCCTTCTCTACAACTGAAAGACATACAGTGGGAGAAGTTATTAAATTAGGTCCTAATGGATCTCCAAGAAGAGCCTGGGGGAAAAGTCCGACAGATTCTGTTCTAAAGATACTTGGAGAAGCTGAACTACAACTTCAGACAGAACTATTAGAAAATACAACTATTAGAAGTGAGATTTCTCCCGAAGGGGAAAAGTACAAACCCTTAATTACTGGAGAAAAAAAAGTACAATGTATTTCACATGAAATAAACCCATCAGCTATTGTTGATTCTCCTGTTGAGACAAAAAGTCCCGAGTTCAGTGAGGCATCTCCACAGATGTCATTGAAACTGGAAGGAAATTTAGAAGAACCTGATGATTTGGAAACAGAAATTCTACAAGAGCCAAGTGGAACAAACAAAGATGAGAGCTTGCCATGCACTATTACTGATGTGTGGATTAGTGAGGAAAAAGAAACAAAGGAAACTCAGTCGGCAGATAGGATCACCATTCAGGAAAATGAAGTTTCTGAAGATGGAGTCTCGAGTACTGTGGACCAACTTAGTGACATTCATATAGAGCCTGGAACCAATGATTCTCAGCACTCTAAATGTGATGTAGATAAGTCTGTGCAACCGGAACCATTTTTCCATAAGGTGGTTCATTCTGAACACTTGAACTTAGTCCCTCAAGTTCAATCAGTTCAGTGTTCACCAGAAGAATCCTTTGCATTTCGATCTCACTCGCATTTACCACCAAAAAATAAAAACAAGAATTCCTTGCTGATTGGACTTTCAACTGGTCTGTTTGATGCAAACAACCCAAAGATGTTAAGGACATGTTCACTTCCAGATCTCTCAAAGCTGTTCAGAACCCTTATGGATGTTCCCACCGTAGGAGATGTTCGTCAAGACAATCTTGAAATAGATGAAATTGAAGATGAAAACATTAAAGAAGGACCTTCTGATTCTGAAGACATTGTGTTTGAAGAAACTGACACAGATTTACAAGAGCTGCAGGCCTCGATGGAACAGTTACTTAGGGAACAACCTGGTGAAGAATACAGTGAAGAAGAAGAGTCAGTCTTGAAGAACAGTGATGTGGAGCCAACTGCAAATGGGACAGATGTGGCAGATGAAGATGACAATCCCAGCAGTGAAAGTGCCCTGAACGAAGAATGGCACTCAGATAACAGTGATGGTGAAATTGCTAGTGAATGTGAATGCGATAGTGTCTTTAACCATTTAGAGGAACTGAGACTTCATCTGGAGCAGGAAATGGGCTTTGAAAAATTCTTTGAGGTTTATGAGAAAATAAAGGCTATTCATGAAGATGAAGATGAAAATATTGAAATTTGTTCAAAAATAGTTCAAAATATTTTGGGAAATGAACATCAGCATCTTTATGCCAAGATTCTTCATTTAGTCATGGCAGATGGAGCCTACCAAGAAGATAATGATGAATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEKYVRLQKIGEGSFGKAILVKSTEDGRQYVIKEINISRMSSKEREESRREVAVLANMKHPNIVQYRESFEENGSLYIVMDYCEGGDLFKRINAQKGVLFQEDQILDWFVQICLALKHVHDRKILHRDIKSQNIFLTKDGTVQLGDFGIARVLNSTVELARTCIGTPYYLSPEICENKPYNNKSDIWALGCVLYELCTLKHAFEAGSMKNLVLKIISGSFPPVSLHYSYDLRSLVSQLFKRNPRDRPSVNSILEKGFIAKRIEKFLSPQLIAEEFCLKTFSKFGSQPIPAKRPASGQNSISVMPAQKITKPAAKYGIPLAYKKYGDKKLHEKKPLQKHKQAHQTPEKRVNTGEERRKISEEAARKRRLEFIEKEKKQKDQIISLMKAEQMKRQEKERLERINRAREQGWRNVLSAGGSGEVKAPFLGSGGTIAPSSFSSRGQYEHYHAIFDQMQQQRAEDNEAKWKREIYGRGLPERGILPGVRPGFPYGAAGHHHFPDADDI*

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU55526
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: inserted stop after 503
• Low Temp: No