cDNA Clone PTPN11

• Synonyms: CS3, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, PTPN11, VHL
• Plasmid: pRETROX TIGHT PURO VHL aCS3
• Parent Plasmid: pRetroX tight puro
• Expressed: VHL-aCS3 (PTPN11) monobody
• Tag: FLAG
• Species: Human

Sequence of Insert

gtggtaccgccatgccccggagggcggagaactgggacgaggccgaggtaggcgcggaggaggcaggcgtcgaagagtacggccctgaagaagacggcggggaggagtcgggcgccgaggagtccggcccggaagagtccggcccggaggaactgggcgccgaggaggagatggaggccggTcggccgcggcccgtgctgcgctcggtgaactcgcgcgagccctcccaggtcatcttctgcaatcgcagtccgcgcgtcgtgctgcccgtatggctcaacttcgacggcgagccgcagccctacccaacgctgccgcctggcacgggccgccgcatccacagctaccgaggtcacctttggctcttcagagatgcagggacacacgatgggcttctggttaaccaaactgaattatttgtgccatctctcaatgttgacggacagcctatttttgccaatatcacactgccagtgtatactctgaaagagcgatgcctccaggttgtccggagcctagtcaagcctgagaattacaggagactggacatcgtcaggtcgctctacgaagatctggaagaccacccaaatgtgcagaaagacctggagcggctgacacaggagcgcattgcacatcaacggatgggagatggtggaggcggaggtgaattcgccatggtgagcagcgtgccgaccaaactggaagtggtggcggcgaccccgaccagcctgctgattagctgggatgcgccggcggtgaccgtggattattatgtgattacctatggcgaaaccggccattggccgtgggtgtggcaggaatttgaagtgccgggcagcaaaagcaccgcgaccattagcggcctgaaaccgggcgtggattataccattaccgtgtatgcgggcagctatagcagctattattattatggcagcccgattagcattaactatcgcacctgagcggccgc

Amino Acid Sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPT
LPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLER
LTQERIAHQRMGDGGGGGEFAMVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGHWPWVWQEFEVPGSKSTATISGLKPGVDYTITVYAGSY
SSYYYYGSPISINYRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRETROX TIGHT PURO VHL aCS3101.6 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU57058
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CS3 anti-PTN11 monobody (modified fibronectin III domain) for use in degron work. Codon optimised.
• Low Temp: No