cDNA Clone PTPN11

• Synonyms: NSA1, BPTP3, CFC, JMML, METCDS, NS1, PTP-1D, PTP2C, SH-PTP2, SH-PTP3, SHP2, PTPN11, VHL
• Plasmid: pRETROX TIGHT PURO VHL aNSA1
• Parent Plasmid: pRetroX tight puro
• Expressed: VHL-aNSA1
• Species: Human

Sequence of Insert

gtggtaccgccatgccccggagggcggagaactgggacgaggccgaggtaggcgcggaggaggcaggcgtcgaagagtacggccctgaagaagacggcggggaggagtcgggcgccgaggagtccggcccggaagagtccggcccggaggaactgggcgccgaggaggagatggaggccggTcggccgcggcccgtgctgcgctcggtgaactcgcgcgagccctcccaggtcatcttctgcaatcgcagtccgcgcgtcgtgctgcccgtatggctcaacttcgacggcgagccgcagccctacccaacgctgccgcctggcacgggccgccgcatccacagctaccgaggtcacctttggctcttcagagatgcagggacacacgatgggcttctggttaaccaaactgaattatttgtgccatctctcaatgttgacggacagcctatttttgccaatatcacactgccagtgtatactctgaaagagcgatgcctccaggttgtccggagcctagtcaagcctgagaattacaggagactggacatcgtcaggtcgctctacgaagatctggaagaccacccaaatgtgcagaaagacctggagcggctgacacaggagcgcattgcacatcaacggatgggagatggtggaggcggaggtgaattcgccatggtgagcagcgtgccgaccaaactggaagtggtggcggcgaccccgaccagcctgctgattagctgggatgcgccggcggtgaccgtggattattatgtgattacctatggcgaaaccggcagcggcggctatgcgtggcaggaatttgaagtgccgggcagcaaaagcaccgcgaccattagcggcctgaaaccgggcgtggattataccattaccgtgtatgcgggctattatggctatccgacctattatagcagcccgattagcattaactatcgcacctgagcggccgc

Amino Acid Sequence

MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPT
LPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLER
LTQERIAHQRMGDGGGGGEFAMVSSVPTKLEVVAATPTSLLISWDAPAVTVDYYVITYGETGSGGYAWQEFEVPGSKSTATISGLKPGVDYTITVYAGYY
GYPTYYSSPISINYRT*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRETROX TIGHT PURO VHL aNSA198.31 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU57059
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Anti-NSA1 (PTPN11) monobody (modified fibronectin III domain) for use in degron work.
• Low Temp: No