cDNA Clone FAM83G

• Plasmid: pGEX6P-1-FAM83G A255R
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83H A255R
• Genbank: NM_198488.3
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAA
CCATGTGAACTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCG
AGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCC
TTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCT
GGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTG
AGGACCCTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTC
GGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGA
GCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCC
CGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACC
CGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAA
AGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGG
TCATGGACATGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCC
GGCTTCAAGAGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGT
CAAGTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACC
TCAAGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGG
TCGGCAACCAAGTTCAAGGGTGCCCTGagaCAGAAGTTCATGTTTGTGGA
TGGAGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGC
GGACGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATG
TTTGACCGGCAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCT
CAAGGGCATCCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGC
CCTCTGTGGTCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTC
AACCCCAAGTACGCACTTGTCAAGGCCAAGAGCGTCGACGAGATTGCCAA
GATCTCCTCTGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCC
CAGCGCTGGCTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATC
CACCCAGGACTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCC
CACGTGGGTGGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACA
TCAATATCATCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAAC
CGCATCAAGATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTG
GAAGCAGAGCCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAG
AGCCCAGTGCCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAG
GGGGACCCTGAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGT
GGCAGATGTACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGC
TCCCCAAAGAGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGG
ATGGCAGGCCCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCA
GGATGACCCCGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGG
TGGAAGAAGAAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGC
CACTCAGGCAGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGC
TTCCTCTGTGTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTC
TCCGGAGGCGCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCG
CGCCGGCAGCTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACC
CCAGGGTGGCTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATG
CGTTGAAGAGGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGC
CAGCAGTTTCATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGA
CGGCTTCCCAGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGA
GCTCTACCAGAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAG
GCCAAGGGAGGTCAGGTACCCCGCCTGCTTCCGGAcCCCGGCAGCCCAAG
ACTGGCCCAAAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGC
ATCCGAGTCCCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCAC
CTAAAGGCCAGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACG
GAGGGCTCAAGCCCCCCGGGACCGCAAAGACCCCtaggcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAFSQVQCLDDNHVNW
RSSESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIRDFLSELELKR
ILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPS
LEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVR
KMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFL
HMCERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALRQKFMFVDGDRA
VCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIP
MEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKSVDEIAKISSE
KQEAKKPLGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVE
PDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQ
DSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVL
ARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPE
SLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVS
EEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGS
PWAQSRGREEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPG
PPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQN
ARPMTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQA
PRDRKDP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU28836
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: can be excised Bam-Not or EcoR1-Not
• Low Temp: No