cDNA Clone NCK2

• Plasmid: pcDNA5-FRT/TO-FLAG-NCK2 R311M
• Parent Plasmid: pcDNA5-FRT/TO-FLAG
• Expressed: FLAG-NCK2 R311M
• Genbank: NM_003581.4 → NP_003572.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGC
CCAGCAGGACCAGGAGCTGGACATCAAGAAGAACGAGCGGCTGTGGTTGC
TGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAGGACG
GGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGG
CTCCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCA
GGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAG
TACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCC
GGCCTTCGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCC
TGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCGACGGTTGG
TGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGT
CTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCC
TGCGCAAGGGCGCCTCGCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCAT
GTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACCGAGGAGGAGCTCAA
CTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACC
CCGAGTGGTGGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCC
AAAAACTACGTGGTGGTCCTCAGTGACGGGCCTGCCCTGCACCCTGCGCA
CGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTCGCGG
GCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCC
CTCAACGAGCGGGGCGTGGAGGGCGACTTCCTCATTAtGGACAGCGAGTC
CTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAAC
ACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGC
TTCCACACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTT
CACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGCAGtgag
cggccgc

Amino Acid Sequence

MDYKDDDDKGSMTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWW
RVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDA
SPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVT
VMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLS
NGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKN
ARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGN
VTRHQAECALNERGVEGDFLIMDSESSPSDFSVSLKASGKNKHFKVQLVD
NVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28935
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cytoplasmic protein NCK2 isoform A.
• Low Temp: No