cDNA Clone NCK2

• Plasmid: pcDNA5-FRT/TO-FLAG-NCK2 W235K
• Parent Plasmid: pcDNA5-FRT/TO-FLAG
• Expressed: FLAG-NCK2 W235K
• Genbank: NM_003581.4 → NP_003572.2
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatccATGACAGAAGAAGTTATTGTGATAGCCAAGTGGGACTACACCGC
CCAGCAGGACCAGGAGCTGGACATCAAGAAGAACGAGCGGCTGTGGTTGC
TGGACGACTCCAAGACGTGGTGGCGGGTGAGGAACGCGGCCAACAGGACG
GGCTATGTACCGTCCAACTACGTGGAGCGGAAGAACAGCCTGAAGAAGGG
CTCCCTCGTGAAGAACCTGAAGGACACACTAGGCCTCGGCAAGACGCGCA
GGAAGACCAGCGCGCGGGATGCGTCCCCCACGCCCAGCACGGACGCCGAG
TACCCCGCCAATGGCAGCGGCGCCGACCGCATCTACGACCTCAACATCCC
GGCCTTCGTCAAGTTCGCCTATGTGGCCGAGCGGGAGGATGAGTTGTCCC
TGGTGAAGGGGTCGCGCGTCACCGTCATGGAGAAGTGCAGCGACGGTTGG
TGGCGGGGCAGCTACAACGGGCAGATCGGCTGGTTCCCCTCCAACTACGT
CTTGGAGGAGGTGGACGAGGCGGCTGCGGAGTCCCCAAGCTTCCTGAGCC
TGCGCAAGGGCGCCTCGCTGAGCAATGGCCAGGGCTCCCGCGTGCTGCAT
GTGGTCCAGACGCTGTACCCCTTCAGCTCAGTCACCGAGGAGGAGCTCAA
CTTCGAGAAGGGGGAGACCATGGAGGTGATTGAGAAGCCGGAGAACGACC
CCGAGTGGaaGAAATGCAAAAATGCCCGGGGCCAGGTGGGCCTCGTCCCC
AAAAACTACGTGGTGGTCCTCAGTGACGGGCCTGCCCTGCACCCTGCGCA
CGCCCCACAGATAAGCTACACCGGGCCCTCGTCCAGCGGGCGCTTCGCGG
GCAGAGAGTGGTACTACGGGAACGTGACGCGGCACCAGGCCGAGTGCGCC
CTCAACGAGCGGGGCGTGGAGGGCGACTTCCTCATTAGGGACAGCGAGTC
CTCGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAAC
ACTTCAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGC
TTCCACACCATGGACGAGCTGGTGGAACACTACAAAAAGGCGCCCATCTT
CACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGCAGtgag
cggccgc

Amino Acid Sequence

MDYKDDDDKGSMTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWW
RVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDA
SPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVT
VMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLS
NGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWKKCKN
ARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGN
VTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVD
NVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU28938
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: cytoplasmic protein NCK2 isoform A.
• Low Temp: No