cDNA Clone FAM83G

• Plasmid: pGEX6P-1-FAM83G S356A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-FAM83G S356A
• Genbank: NM_001039999.2
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCCCGGAATTCATGGCCTTCTCTCAGGTGCAGTGTCTGGACGACAACCATGTGAACTGGCGCTCCAGCGAGTCCAAGCCTGAGTTCTTCTACAGCG
AGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCT
GGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTC
GGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCC
CGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAA
AGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCC
GGCTTCAAGAGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACC
TCAAGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGA
TGGAGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATG
TTTGACCGGCAGTTCCAGGAGCTGTACCTCATGTCACACAGTGTGAGCCTCAAGGGCATCCCTATGGAGAAGGAACCGGAGCCGGAGCCTATTGTGCTGC
CCTCTGTGGTCCCCCTGGTGCCCGCGGGCACTGTGGCCAAGAAGCTCGTCAACCCCAAGTACGCACTTGTCAAGGCCAAGgccGTCGACGAGATTGCCAA
GATCTCCTCTGAGAAGCAGGAGGCCAAGAAGCCCCTGGGGCTGAAAGGCCCAGCGCTGGCTGAGCATCCAGGGGAACTCCCCGAGCTGCTGCCACCCATC
CACCCAGGACTGCTTCACCTGGAGAGGGCCAACATGTTTGAGTACCTGCCCACGTGGGTGGAGCCAGACCCGGAGCCTGGCAGCGACATCCTGGGCTACA
TCAATATCATCGACCCCAACATCTGGAACCCCCAGCCCAGCCAGATGAACCGCATCAAGATCCGTGACACCTCCCAGGCCAGCGCCCAGCACCAGCTGTG
GAAGCAGAGCCAGGACAGCAGGCCCCGTCCAGAGCCTTGCCCTCCCCCAGAGCCCAGTGCCCCCCAGGACGGTGTCCCAGCTGAGAACGGCCTCCCCCAG
GGGGACCCTGAGCCATTGCCCCCCGTGCCCAAGCCCCGGACAGTCCCTGTGGCAGATGTACTAGCCCGGGACAGCAGTGATATTGGCTGGGTCCTGGAGC
TCCCCAAAGAGGAAGCTCCCCAGAATGGGACAGACCATAGGCTACCCAGGATGGCAGGCCCAGGCCACGCCCCACTCCAGCGGCAGCTATCTGTGACCCA
GGATGACCCCGAGAGCCTCGGGGTGGGGCTCCCCAATGGGCTGGATGGGGTGGAAGAAGAAGATGATGACGACTACGTAACCCTCAGTGACCAGGACAGC
CACTCAGGCAGCTCCGGCCGTGGCCCTGGCCCCCGACGGCCCTCAGTGGCTTCCTCTGTGTCAGAGGAGTACTTCGAGGTGAGAGAGCACTCAGTCCCTC
TCCGGAGGCGCCACTCAGAGCAAGTGGCCAACGGGCCAACCCCACCACCGCGCCGGCAGCTGAGTGCCCCCCATATAACCCGAGGGACCTTTGTTGGACC
CCAGGGTGGCTCCCCATGGGCCCAGAGTCGGGGAAGAGAAGAAGCAGATGCGTTGAAGAGGATGCAGGCCCAGCGCTCCACAGACAAGGAGGCACAGGGC
CAGCAGTTTCATCATCACAGGGTCCCTGCCTCAGGGACTAGGGATAAAGACGGCTTCCCAGGACCCCCTAGGTACCGCTCTGCTGCTGACAGCGTCCAGA
GCTCTACCAGAAACGCTGGCCCAGCCATGGCTGGCCCCCACCACTGGCAGGCCAAGGGAGGTCAGGTACCCCGCCTGCTTCCGGAcCCCGGCAGCCCAAG
ACTGGCCCAAAATGCCCGCCCCATGACCGATGGCAGGGCCACCGAGGAGCATCCGAGTCCCTTCGGAATCCCATACTCCAAACTGTCTCAGTCGAAGCAC
CTAAAGGCCAGGACGGGCGGTAGCCAGTGGGCCTCATCGGATTCTAAACGGAGGGCTCAAGCCCCCCGGGACCGCAAAGACCCCTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEFMAFSQVQCLDDNHVNWRSSESKPEFFYSEEQRLALEALVARGRDAFYEVLKRENIRDFLSELELKR
ILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGVTRASVYMQPPIDGQAHIKEVVR
KMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFTRSATKFKGALAQKFMFVDGDRA
VCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMSHSVSLKGIPMEKEPEPEPIVLPSVVPLVPAGTVAKKLVNPKYALVKAKAVDEIAKISSE
KQEAKKPLGLKGPALAEHPGELPELLPPIHPGLLHLERANMFEYLPTWVEPDPEPGSDILGYINIIDPNIWNPQPSQMNRIKIRDTSQASAQHQLWKQSQ
DSRPRPEPCPPPEPSAPQDGVPAENGLPQGDPEPLPPVPKPRTVPVADVLARDSSDIGWVLELPKEEAPQNGTDHRLPRMAGPGHAPLQRQLSVTQDDPE
SLGVGLPNGLDGVEEEDDDDYVTLSDQDSHSGSSGRGPGPRRPSVASSVSEEYFEVREHSVPLRRRHSEQVANGPTPPPRRQLSAPHITRGTFVGPQGGS
PWAQSRGREEADALKRMQAQRSTDKEAQGQQFHHHRVPASGTRDKDGFPGPPRYRSAADSVQSSTRNAGPAMAGPHHWQAKGGQVPRLLPDPGSPRLAQN
ARPMTDGRATEEHPSPFGIPYSKLSQSKHLKARTGGSQWASSDSKRRAQAPRDRKDP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU29131
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 44023. Silent change at D757 (GAT > GAC) compared to NCBI
• Low Temp: No