cDNA Clone Nek10

• Synonyms: JCK; NEK12A; NPHP9; RHPD2
• Plasmid: pcDNA5 FRT/TO GFP Nek10
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP Nek10
• Genbank: NM_001031741.3 with two silent changes
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGTTCCTGTACAGCCTGCAGAACCCCCCGAGGTACTGAAGAGTGAGCCGTATGGGGAGAAGGCTGATGTCTGGGCAGTAGGCTGCATCCTTTATCAGATGGCGACTTTGAGTCCCCCCTTCTACAGCACTAACATGCTGTCCTTGGCTACAAAAATAGTGGAGGCGGTATATGAACCAGTGCCAGAAGGTATCTACTCTGAAAAAGTAACAGACACCATCAGCAGGTGCCTCACTCCTGATGCGGAAGCTCGTCCAGATATTGTAGAAGTCAGTTCGATGATATCAGATGTCATGATGAAATATTTAGACAACTTATCTACATCCCAGTTGTCCTTGGAAAAGAAGCTAGAACGGGAACGAAGACGCACACAAAGGTATTTTATGGAAGCCAACCGGAACACCGTCACATGTCACCATGAGCTGGCTGTTCTATCTCACGAGACCTTTGAGAAGGCAAGTTTGAGTAGCAGCAGCAGTGGAGCAGCCAGCCTGAAAAGTGAACTTTCAGAAAGCGCAGACCTGCCCCCTGAAGGCTTCCAGGCCTCCTATGGTAAAGACGAAGACAGGGCCTGTGACGAAATCCTGTCAGATGATAACTTCAACCTGGAAAATGCTGAGAAAGATACATATTCAGAGGTAGATGATGAATTGGACATTTCGGATAACTCCAGCAGCTCCAGTTCAAGCCCTCTGAAAGAATCTACATTCAACATTTTAAAGAGAAGTTTTAGTGCTTCAGGAGGAGAAAGACAATCCCAAACAAGGGACTTCACTGGAGGAACAGGATCAAGACCAAGACCAGCTTTGCTGCCTCTTGACCTGCTTCTGAAAGTGCCACCCCATATGCTCAGGGCCCACATTAAGGAAATAGAGGCTGAGTTAGTGACAGGGTGGCAGTCCCATAGCCTTCCTGCTGTGATTCTTCGAAATCTCAAAGATCATGCATCAGCAGGAATTGCTGTGTCCCAGAGGAAAGTGCGTCAGATCAGTGATCCTATTCAGCAGATATTAATTCAGCTGCACAAAATAATCTATATCACACAGCTTCCTCCAGCTTTGCACCACAATTTGAAAAGAAGGGTTATAGAGAGATTCAAGAAATCCCTCTTCAGCCAGCAGAGTAACCCTTGTAATTTGAAATCTGAAATTAAAAAGTTATCTCAGGGATCTCCAGAACCGATTGAGCCCAACTTTTTCACAGCAGATTACCATTTATTACATCGTTCATCCGGTGGAAACAGCCTGTCCCCAAATGACCCTACAGGTTTACCAACCAGCATTGAATTGGAGGAAGGAATAACATATGAACAGATGCAGACTGTGATTGAAGAAGTCCTTGAGGAAAGTGGCTATTACAATTTTACATCTAACAGGTATCATTCCTATCCATGGGGGACCAAGAATCACCCAACCAAAAGATGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMVPVQPAEPPEVLKSEPYGEKADVWAVGCILYQMATLSPPFYSTNMLSLATKIVEA
VYEPVPEGIYSEKVTDTISRCLTPDAEARPDIVEVSSMISDVMMKYLDNLSTSQLSLEKKLERERRRTQRYFMEANRNTVTCHHELAVLSHETFEKASLS
SSSSGAASLKSELSESADLPPEGFQASYGKDEDRACDEILSDDNFNLENAEKDTYSEVDDELDISDNSSSSSSSPLKESTFNILKRSFSASGGERQSQTR
DFTGGTGSRPRPALLPLDLLLKVPPHMLRAHIKEIEAELVTGWQSHSLPAVILRNLKDHASAGIAVSQRKVRQISDPIQQILIQLHKIIYITQLPPALHH
NLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIEPNFFTADYHLLHRSSGGNSLSPNDPTGLPTSIELEEGITYEQMQTVIEEVLEESGYYNFT
SNRYHSYPWGTKNHPTKR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU58430
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No