cDNA Clone NAIP (human)

• Plasmid: pCMV5D NAIP
• Parent Plasmid: pSC-B
• Expressed: NAIP
• Genbank: NP_004527.2
• Species: Human

Sequence of Insert

GGATCCATGGCCACCCAGCAGAAAGCCTCTGACGAGAGGATCTCCCAGTTTGATCACAATTTGCTGCCAGAGCTGTCTGCTCTTCTGGGCCTAGATGCAGTTCAGTTGGCAAAGGAACTAGAAGAAGAGGAGCAGAAGGAGCGAGCAAAAATGCAGAAAGGCTACAACTCTCAAATGCGCAGTGAAGCAAAAAGGTTAAAGACTTTTGTGACTTATGAGCCGTACAGCTCATGGATACCACAGGAGATGGCGGCCGCTGGGTTTTACTTCACTGGGGTAAAATCTGGGATTCAGTGCTTCTGCTGTAGCCTAATCCTCTTTGGTGCCGGCCTCACGAGACTCCCCATAGAAGACCACAAGAGGTTTCATCCAGATTGTGGGTTCCTTTTGAACAAGGATGTTGGTAACATTGCCAAGTACGACATAAGGGTGAAGAATCTGAAGAGCAGGCTGAGAGGAGGTAAAATGAGGTACCAAGAAGAGGAGGCTAGACTTGCATCCTTCAGGAACTGGCCATTTTATGTCCAAGGGATATCCCCTTGTGTGCTCTCAGAGGCTGGCTTTGTCTTTACAGGTAAACAGGACACGGTACAGTGTTTTTCCTGTGGTGGATGTTTAGGAAATTGGGAAGAAGGAGATGATCCTTGGAAGGAACATGCCAAATGGTTCCCCAAATGTGAATTTCTTCGGAGTAAGAAATCCTCAGAGGAAATTACCCAGTATATTCAAAGCTACAAGGGATTTGTTGACATAACGGGAGAACATTTTGTGAATTCCTGGGTCCAGAGAGAATTACCTATGGCATCAGCTTATTGCAATGACAGCATCTTTGCTTACGAAGAACTACGGCTGGACTCTTTTAAGGACTGGCCCCGGGAATCAGCTGTGGGAGTTGCAGCACTGGCCAAAGCAGGTCTTTTCTACACAGGTATAAAGGACATCGTCCAGTGCTTTTCCTGTGGAGGGTGTTTAGAGAAATGGCAGGAAGGTGATGACCCATTAGACGATCACACCAGATGTTTTCCCAATTGTCCATTTCTCCAAAATATGAAGTCCTCTGCGGAAGTGACTCCAGACCTTCAGAGCCGTGGTGAACTTTGTGAATTACTGGAAACCACAAGTGAAAGCAATCTTGAAGATTCAATAGCAGTTGGTCCTATAGTGCCAGAAATGGCACAGGGTGAAGCCCAGTGGTTTCAAGAGGCAAAGAATCTGAATGAGCAGCTGAGAGCAGCTTATACCAGCGCCAGTTTCCGCCACATGTCTTTGCTTGATATCTCTTCCGATCTGGCCACGGACCACTTGCTGGGCTGTGATCTGTCTATTGCTTCAAAACACATCAGCAAACCTGTGCAAGAACCTCTGGTGCTGCCTGAGGTCTTTGGCAACTTGAACTCTGTCATGTGTGTGGAGGGTGAAGCTGGAAGTGGAAAGACGGTCCTCCTGAAGAAAATAGCTTTTCTGTGGGCATCTGGATGCTGTCCCCTGTTAAACAGGTTCCAGCTGGTTTTCTACCTCTCCCTTAGTTCCACCAGACCAGACGAGGGGCTGGCCAGTATCATCTGTGACCAGCTCCTAGAGAAAGAAGGATCTGTTACTGAAATGTGCATGAGGAACATTATCCAGCAGTTAAAGAATCAGGTCTTATTCCTTTTAGATGACTACAAAGAAATATGTTCAATCCCTCAAGTCATAGGAAAACTGATTCAAAAAAACCACTTATCCCGGACCTGCCTATTGATTGCTGTCCGTACAAACAGGGCCAGGGACATCCGCCGATACCTAGAGACCATTCTAGAGATCAAAGCATTTCCCTTTTATAATACTGTCTGTATATTACGGAAGCTCTTTTCACATAATATGACTCGTCTGCGAAAGTTTATGGTTTACTTTGGAAAGAACCAAAGTTTGCAGAAGATACAGAAAACTCCTCTCTTTGTGGCGGCGATCTGTGCTCATTGGTTTCAGTATCCTTTTGACCCATCCTTTGATGATGTGGCTGTTTTCAAGTCCTATATGGAACGCCTTTCCTTAAGGAACAAAGCGACAGCTGAAATTCTCAAAGCAACTGTGTCCTCCTGTGGTGAGCTGGCCTTGAAAGGGTTTTTTTCATGTTGCTTTGAGTTTAATGATGATGATCTCGCAGAAGCAGGGGTTGATGAAGATGAAGATCTAACCATGTGCTTGATGAGCAAATTTACAGCCCAGAGACTAAGACCATTCTACCGGTTTTTAAGTCCTGCCTTCCAAGAATTTCTTGCGGGGATGAGGCTGATTGAACTCCTGGATTCAGATAGGCAGGAACATCAAGATTTGGGACTGTATCATTTGAAACAAATCAACTCACCCATGATGACTGTAAGCGCCTACAACAATTTTTTGAACTATGTCTCCAGCCTCCCTTCAACAAAAGCAGGGCCCAAAATTGTGTCTCATTTGCTCCATTTAGTGGATAACAAAGAGTCATTGGAGAATATATCTGAAAATGATGACTACTTAAAGCACCAGCCAGAAATTTCACTGCAGATGCAGTTACTTAGGGGATTGTGGCAAATTTGTCCACAAGCTTACTTTTCAATGGTTTCAGAACATTTACTGGTTCTTGCCCTGAAAACTGCTTATCAAAGCAACACTGTTGCTGCGTGTTCTCCATTTGTTTTGCAATTCCTTCAAGGGAGAACACTGACTTTGGGTGCGCTTAACTTACAGTACTTTTTCGACCACCCAGAAAGCTTGTCATTGTTGAGGAGCATCCACTTCCCAATACGAGGAAATAAGACATCACCCAGAGCACATTTTTCAGTTCTGGAAACATGTTTTGACAAATCACAGGTGCCAACTATAGATCAGGACTATGCTTCTGCCTTTGAACCTATGAATGAATGGGAGCGAAATTTAGCTGAAAAAGAGGATAATGTAAAGAGCTATATGGATATGCAGCGCAGGGCATCACCAGACCTTAGTACTGGCTATTGGAAACTTTCTCCAAAGCAGTACAAGATTCCCTGTCTAGAAGTCGATGTGAATGATATTGATGTTGTAGGCCAGGATATGCTTGAGATTCTAATGACAGTTTTCTCAGCTTCACAGCGCATCGAACTCCATTTAAACCACAGCAGAGGCTTTATAGAAAGCATCCGCCCAGCTCTTGAGCTGTCTAAGGCCTCTGTCACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAACTGCTTCTCACCCTGCCTTCCCTGGAATCTCTTGAAGTCTCAGGGACAATCCAGTCACAAGACCAAATCTTTCCTAATCTGGATAAGTTCCTGTGCCTGAAAGAACTGTCTGTGGATCTGGAGGGCAATATAAATGTTTTTTCAGTCATTCCTGAAGAATTTCCAAACTTCCACCATATGGAGAAATTATTGATCCAAATTTCAGCTGAGTATGATCCTTCCAAACTAGTAAAATTAATTCAAAATTCTCCAAACCTTCATGTTTTCCATCTGAAGTGTAACTTCTTTTCGGATTTTGGGTCTCTCATGACTATGCTTGTTTCCTGTAAGAAACTCACAGAAATTAAGTTTTCGGATTCATTTTTTCAAGCCGTCCCATTTGTTGCCAGTTTGCCAAATTTTATTTCTCTGAAGATATTAAATCTTGAAGGCCAGCAATTTCCTGATGAGGAAACATCAGAAAAATTTGCCTACATTTTAGGTTCTCTTAGTAACCTGGAAGAATTGATCCTTCCTACTGGGGATGGAATTTATCGAGTGGCCAAACTGATCATCCAGCAGTGTCAGCAGCTTCATTGTCTCCGAGTCCTCTCATTTTTCAAGACTTTGAATGATGACAGCGTGGTGGAAATTGCCAAAGTAGCAATCAGTGGAGGTTTCCAGAAACTTGAGAACCTAAAGCTTTCAATCAATCACAAGATTACAGAGGAAGGATACAGAAATTTCTTTCAAGCACTGGACAACATGCCAAACTTGCAGGAGTTGGACATCTCCAGGCATTTCACAGAGTGTATCAAAGCTCAGGCCACAACAGTCAAGTCTTTGAGTCAATGTGTGTTACGACTACCAAGGCTCATTAGACTGAACATGTTAAGTTGGCTCTTGGATGCAGATGATATTGCATTGCTTAATGTCATGAAAGAAAGACATCCTCAATCTAAGTACTTAACTATTCTCCAGAAATGGATACTGCCGTTCTCTCCAATCATTCAGAAATAACTCGAG

Amino Acid Sequence

MATQQKASDERISQFDHNLLPELSALLGLDAVQLAKELEEEEQKERAKMQKGYNSQMRSEAKRLKTFVTYEPYSSWIPQEMAAAGFYFTGVKSGIQCFCCSLILFGAGLTRLPIEDHKRFHPDCGFLLNKDVGNIAKYDIRVKNLKSRLRGGKMRYQEEEARLASFRNWPFYVQGISPCVLSEAGFVFTGKQDTVQCFSCGGCLGNWEEGDDPWKEHAKWFPKCEFLRSKKSSEEITQYIQSYKGFVDITGEHFVNSWVQRELPMASAYCNDSIFAYEELRLDSFKDWPRESAVGVAALAKAGLFYTGIKDIVQCFSCGGCLEKWQEGDDPLDDHTRCFPNCPFLQNMKSSAEVTPDLQSRGELCELLETTSESNLEDSIAVGPIVPEMAQGEAQWFQEAKNLNEQLRAAYTSASFRHMSLLDISSDLATDHLLGCDLSIASKHISKPVQEPLVLPEVFGNLNSVMCVEGEAGSGKTVLLKKIAFLWASGCCPLLNRFQLVFYLSLSSTRPDEGLASIICDQLLEKEGSVTEMCMRNIIQQLKNQVLFLLDDYKEICSIPQVIGKLIQKNHLSRTCLLIAVRTNRARDIRRYLETILEIKAFPFYNTVCILRKLFSHNMTRLRKFMVYFGKNQSLQKIQKTPLFVAAICAHWFQYPFDPSFDDVAVFKSYMERLSLRNKATAEILKATVSSCGELALKGFFSCCFEFNDDDLAEAGVDEDEDLTMCLMSKFTAQRLRPFYRFLSPAFQEFLAGMRLIELLDSDRQEHQDLGLYHLKQINSPMMTVSAYNNFLNYVSSLPSTKAGPKIVSHLLHLVDNKESLENISENDDYLKHQPEISLQMQLLRGLWQICPQAYFSMVSEHLLVLALKTAYQSNTVAACSPFVLQFLQGRTLTLGALNLQYFFDHPESLSLLRSIHFPIRGNKTSPRAHFSVLETCFDKSQVPTIDQDYASAFEPMNEWERNLAEKEDNVKSYMDMQRRASPDLSTGYWKLSPKQYKIPCLEVDVNDIDVVGQDMLEILMTVFSASQRIELHLNHSRGFIESIRPALELSKASVTKCSISKLELSAAEQELLLTLPSLESLEVSGTIQSQDQIFPNLDKFLCLKELSVDLEGNINVFSVIPEEFPNFHHMEKLLIQISAEYDPSKLVKLIQNSPNLHVFHLKCNFFSDFGSLMTMLVSCKKLTEIKFSDSFFQAVPFVASLPNFISLKILNLEGQQFPDEETSEKFAYILGSLSNLEELILPTGDGIYRVAKLIIQQCQQLHCLRVLSFFKTLNDDSVVEIAKVAISGGFQKLENLKLSINHKITEEGYRNFFQALDNMPNLQELDISRHFTECIKAQATTVKSLSQCVLRLPRLIRLNMLSWLLDADDIALLNVMKERHPQSKYLTILQKWILPFSPIIQK*

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU27785
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Has non silent mutation to be corrected V535M. 1 silent mutation A164
• Low Temp: No