cDNA Clone KIAA1164

• Plasmid: pGEX6P1 KIAA1164 Y243A C266A
• Parent Plasmid: pGEX6P1
• Expressed: GST KIAA1164 Y243A C266A
• Tag: GST

Sequence of Insert

AGATCTATGGAGAGCAGCCCCGAGAGCCTGCAGCCGCTAGAACACGGGGTGGCGGCCGGGCCAGCGTCAGGGACAGGTTCTTCGCAGGAAGGGCTACAGGAGACCAGGCTCGCCGCTGGTGATGGTCCTGGGGTATGGGCGGCGGAGACCAGCGGCGGGAATGGGCTGGGGGCGGCGGCCGCCAGGAGGAGCCTCCCGGACTCGGCTTCTCCCGCGGGCTCTCCTGAGGTTCCCGGACCCTGCAGCTCCTCCGCGGGTTTGGACTTGAAGGACAGTGGTTTGGAGAGTCCTGCTGCCGCCGAGGCGCCTCTGAGAGGGCAGTACAAGGTGACCGCCTCCCCGGAGACAGCCGTGGCCGGAGTGGGTCATGAGTTGGGTACCGCCGGAGACGCGGGAGCCCGCCCGGATCTCGCCGGCACCTGCCAAGCAGAACTGACCGCCGCCGGCTCCGAAGAGCCCAGCAGCGCCGGCGGCCTCAGCAGCAGTTGCAGCGACCCGAGCCCTCCTGGGGAATCTCCGAGCCTGGACTCTCTGGAGTCGTTCTCTAACCTGCATTCTTTTCCCAGTAGCTGCGAGTTCAATAGTGAGGAGGGAGCGGAGAACAGGGTCCCTGAGGAGGAGGAGGGCGCGGCGGTGTTGCCCGGGGCTGTTCCTCTGTGCAAGGAGGAGGAGGGGGAGGAGACCGCTCAGGTGCTGGCGGCCTCCAAGGAACGCTTCCCGGGACAATCTGTGGCTCACATCAAGTGGATCCAGTGGAAGGAAGAGAACACACCCATCATCACCCAGAATGAGAACGGACCCGCTCCCTTGCTGGCCATCCTCAATGTTTTGCTCCTGGCCTGGAAGGTGAAACTTCCACCGATGATGGAAATCATAACTGCTGAGCAGCTGATGGAATATTTAGGAGATTACATGCTTGATGCAAAGCCAAAAGAAATTTCAGAAATTCAACGTTTAAATTATGAACAGAATATGAGTGATGCCATGGCAATTTTGCACAAACTACAGACAGGCCTGGATGTAAATGTAAGATTCACTGGTGTTCGAGTGTTTGAATATACACCAGAATGCATAGTATTTGATCTTCTTGATATTCCTTTGTACCATGGGTGGTTAGTAGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGTAACTGCAGCTACAACCAACTAGTGGAGAAGATCATCTCTTGTAAACAGTCAGACAATAGTGAGCTGGTTAGTGAAGGCTTTGTAGCTGAGCAGTTTCTAAATAACACAGCCACTCAACTGACATACCATGGATTATGTGAACTAACTTCAACGGTTCAGGAAGGAGAACTTTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACCAAATACAAGGGTCAACTGTATTTGTTGGTAACGGACCAGGGGTTTCTTACTGAAGAGAAAGTTGTTTGGGAAAGCCTACACAACGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTTCATCTTCGACCTCCTTCAGATCCTGAAACTGTATACAAAGGACAACAAGATCAGATAGATCAGGATTATCTTATGGCATTATCTCTACAACAAGAACAGCAGAGCCAAGAGATCAATTGGGAACAAATCCCGGAAGGAATCAGTGATTTGGAACTAGCAAAGAAACTCCAAGAGGAAGAGGACAGACGGGCTTCTCAATACTATCAGGAACAGGAACAAGCAGCAGCTGCTGCTGCTGCTGCTTCTACACAGGCTCAGCAGGGCCAGCCAGCACAAGCCTCTCCATCAAGTGGAAGACAATCTGGGAATAGTGAACGTAAACGGAAGGAACCACGAGAAAAAGATAAAGAAAAAGAAAAGGAAAAAAATAGCTGTGTTATTTTGTAAGATCCCCGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMESSPESLQPLEHGVAAGPASGTGSSQEGLQETRLAAGDGPGVWAAETSGGNGLGAAAARRSLPDSASPAGSPEVPGPCSSSAGLDLKDSGLESPAAAEAPLRGQYKVTASPETAVAGVGHELGTAGDAGARPDLAGTCQAELTAAGSEEPSSAGGLSSSCSDPSPPGESPSLDSLESFSNLHSFPSSCEFNSEEGAENRVPEEEEGAAVLPGAVPLCKEEEGEETAQVLAASKERFPGQSVAHIKWIQWKEENTPIITQNENGPAPLLAILNVLLLAWKVKLPPMMEIITAEQLMEYLGDYMLDAKPKEISEIQRLNYEQNMSDAMAILHKLQTGLDVNVRFTGVRVFEYTPECIVFDLLDIPLYHGWLVDPQIDDIVKAVGNCSYNQLVEKIISCKQSDNSELVSEGFVAEQFLNNTATQLTYHGLCELTSTVQEGELCVFFRNNHFSTMTKYKGQLYLLVTDQGFLTEEKVVWESLHNVDGDGNFCDSEFHLRPPSDPETVYKGQQDQIDQDYLMALSLQQEQQSQEINWEQIPEGISDLELAKKLQEEEDRRASQYYQEQEQAAAAAAAASTQAQQGQPAQASPSSGRQSGNSERKRKEPREKDKEKEKEKNSCVIL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU59144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Please mutate Y243 to alanine in 55340
• Low Temp: No