cDNA Clone Shroom4

• Plasmid: pcDNA FRT TO GFP Shroom4 S1089L
• Parent Plasmid: pcDNA FRT TO GFP
• Expressed: GFP Shroom 4 S1089L
• Tag: GFP

Sequence of Insert

GAATTCATGGAGAACCGGCCTGGGTCCTTCCAGTACGTCCCTGTGCAGCTGCAAGGGGGGGCACCCTGGGGCTTCACCCTTAAGGGGGGTCTGGAACACTGTGAGCCGCTCACAGTGTCTAAGATTGAAGATGGAGGCAAGGCAGCTTTGTCCCAGAAGATGAGGACTGGTGATGAGCTGGTGAATATCAATGGCACTCCATTATATGGCTCCCGCCAAGAGGCCCTCATTCTCATCAAAGGCTCCTTCCGGATTCTCAAGCTGATTGTCAGGAGGAGGAACGCCCCTGTCAGTAGGCCGCACTCATGGCATGTGGCCAAGCTGCTGGAGGGATGCCCTGAAGCAGCCACCACCATGCATTTCCCTTCTGAAGCCTTCAGCTTGTCCTGGCATTCTGGCTGCAACACAAGTGACGTGTGTGTGCAGTGGTGTCCACTCTCCCGGCATTGCAGCACCGAGAAAAGCAGCTCCATTGGCAGCATGGAGAGCCTGGAGCAACCAGGCCAAGCCACCTATGAGAGCCATCTGTTGCCTATTGACCAGAACATGTACCCTAACCAGCGTGACTCAGCCTACAGCTCCTTCTCGGCCAGCTCAAATGCTTCTGACTGTGCCCTTTCCCTCAGGCCAGAGGAGCCAGCCTCTACAGACTGCATCATGCAAGGCCCAGGGCCAACTAAGGCCCCCAGTGGCCGGCCTAATGTGGCTGAGACCTCAGGAGGTAGTCGGCGCACCAATGGGGGCCACCTGACCCCCAGCTCTCAGATGTCATCCCGTCCACAGGAGGGATACCAGTCAGGGCCCGCCAAAGCAGTCAGGGGCCCACCACAACCTCCAGTGAGGCGGGACAGCCTTCAGGCCTCCAGAGCCCAACTCCTCAATGGAGAGCAGCGCAGGGCATCTGAGCCTGTGGTCCCCTTGCCACAGAAGGAGAAACTGAGCTTAGAGCCTGTGCTACCCGCAAGGAACCCTAATAGGTTCTGTTGCCTCAGTGGGCATGACCAAGTGACAAGTGAGGGCCATCAGAACTGTGAGTTCAGTCAGCCTCCTGAATCCAGCCAACAGGGCTCTGAGCATCTACTGATGCAGGCCTCAACCAAAGCTGTTGGATCCCCAAAAGCCTGTGACAGAGCTTCCAGCGTGGATTCCAACCCACTCAATGAGGCTTCTGCAGAGCTAGCTAAGGCTTCTTTTGGCAGACCTCCACATCTCATAGGACCCACAGGGCATCGCCATAGTGCCCCTGAACAGCTGCTGGCATCCCACCTGCAGCATGTGCACCTTGATACCAGGGGCAGCAAAGGGATGGAGCTCCCACCCGTACAGGATGGGCACCAGTGGACTCTGTCCCCTTTGCACAGCAGCCACAAAGGGAAGAAAAGTCCATGCCCCCCTACAGGAGGAACCCATGACCAGTCCAGCAAAGAAAGAAAGACCAGACAAGTGGATGACAGGTCTTTAGTTTTGGGACACCAGAGCCAAAGCAGTCCCCCACATGGAGAGGCTGATGGACACCCCTCAGAAAAAGGTTTCCTGGACCCAAACAGAACAAGCAGAGCAGCCAGTGAATTGGCCAACCAGCAACCCTCTGCCTCTGGCTCCCTTGTTCAACAAGCCACGGACTGTTCTTCAACCACTAAAGCAGCTAGTGGCACAGAGGCAGGTGAAGAAGGGGACAGCGAGCCCAAGGAGTGCAGCCGGATGGGTGGTAGGCGAAGTGGAGGGACCCGGGGCCGCTCGATCCAAAACCGGCGGAAGAGTGAGCGTTTTGCTACCAATCTGCGTAATGAAATTCAGAGGAGGAAGGCCCAGCTCCAGAAAAGCAAGGGTCCCTTGTCACAGCTGTGTGACACTAAGGAGCCAGTGGAAGAGACCCAGGAGCCCCCAGAAAGTCCTCCACTCACTGCCTCTAACACATCTCTTCTATCTTCATGTAAAAAACCTCCCAGCCCCAGAGACAAGCTCTTCAACAAAAGCATGATGCTCAGAGCTAGGTCTTCCGAGTGCCTCAGCCAAGCCCCTGAGAGCCATGAATCTAGGACAGGCTTAGAGGGACGAATAAGCCCTGGCCAGAGGCCTGGCCAGTCCTCTTTGGGCCTGAACACCTGGTGGAAAGCACCTGACCCATCCTCCTCAGACCCTGAGAAAGCACATGCTCACTGTGGAGTCCGTGGAGGTCATTGGAGATGGTCTCCAGAGCATAATTCACAGCCACTTGTGGCAGCAGCCATGGAAGGCCCTTCCAACCCAGGTGACAACAAGGAATTGAAGGCTTCTACTGCTCAAGCTGGGGAGGATGCCATCCTCTTGCCTTTTGCAGACAGAAGAAAGTTCTTTGAAGAGAGTAGCAAATCCTTATCTACATCTCATTTGCCAGGTTTAACCACTCATAGCAACAAGACTTTTACCCAGAGACCAAAACCTATAGACCAAAACTTCCAGCCAATGAGCTCCAGCTGTAGGGAATTGAGGCGCCATCCCATGGACCAATCATATCATTCCGCAGACCAACCATATCATGCCACAGACCAATCATATCATTCCATGTCACCCCTTCAGTCAGAAACTCCCACTTACTCAGAATGTTTTGCAAGCAAAGGTCTAGAAAATTCCATGTGTTGTAAGCCACTACACTGTGGTGATTTTGATTACCACAGGACCTGCTCTTACTCCTGCAGTGTTCAAGGAGCTCTAGTCCATGATCCTTGCATTTATTGTTCTGGGGAAATCTGCCCTGCCTTGCTAAAGAGAAATATGATGCCAAATTGCTACAACTGCCGGTGCCACCACCACCAATGCATTCGGTGTTCAGTTTGCTATCATAATCCTCAGCACAGTGCCCTCGAGGACAGCAGCTTGGCACCTGGCAACACTTGGAAACCCAGGAAGCTGACAGTGCAGGAATTTCCTGGGGACAAATGGAATCCAATAACAGGAAACAGGAAGACCAGCCAGTCAGGGAGGGAAATGGCTCATTCCAAGACTAGCTTTTCATGGGCAACCCCTTTCCATCCTTGCCTTGAGAACCCAGCACTGGACTTGTCAAGCTACCGAGCAATTTCTTCTCTTGACCTCCTTGGAGACTTCAAACATGCTTTGAAAAAATCAGAGGAAACTTCAGTTTATGAGGAGGGGAGCTCCCTTGCCTCCATGCCCCACCCACTGCGCAGCCGTGCCTTCTCAGAGAGTCACATCAGCTTGGCGCCCCAAAGCACCCGGGCCTGGGGGCAGCATAGGAGGGAGCTCTTTAGCAAAGGTGATGAGACCCAGTTGGATCTTCTCGGAGCCAGGAAGAAGGCCTTTCCTCCTCCTCGCCCTCCTCCTCCCAACTGGGAGAAGTACAGGCTCTTTCGTGCAGCCCAGCAGCAGAAGCAGCAACAGCAGCAGCAACAGCAGCAGCAGAAGCAACAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAGGAAGAGGAAGAGGAGGAGGAGGAGGCAGAGGAGGAGGAGGAGGAGCTGCCACCCCAGTATTTCAGTTCAGAAACCTCTGGTTCCTGTGCTCTCAATCCTGAGGAGGTCCTAGAGCAGCCACAACCCCTCAGCTTTGGCCACCTGGAGGGCTCGAGACAGGGTTCACAAAGTGTCCCAGCAGAGCAAGAATCCTTTGCACTCCATTCCAGTGATTTCTTGCCTCCAATAAGGGGTCACTTGGGATCTCAACCTGAGCAGGCTCAGCCCCCTTGCTACTATGGCATTGGTGGGCTTTGGAGGACATCGGGACAGGAAGCCACTGAATCCGCCAAACAAGAGTTTCAGCACTTTTCGCCTCCTTCAGGGGCCCCAGGAATCCCTACCTCTTACTCAGCTTATTACAATATTTCTGTGGCCAAGGCAGAGCTGCTGAACAAACTGAAAGACCAACCTGAGATGGCAGAGATTGGCCTAGGAGAGGAGGAAGTTGACCATGAACTGGCTCAAAAAAAGATACAGCTTATCGAAAGCATCAGCAGAAAACTTTCTGTCTTGCGGGAGGCCCAGCGAGGGCTGCTAGAGGACATCAATGCCAATTCTGCCCTTGGGGAGGAGGTGGAGGCCAACTTAAAAGCCGTCTGCAAATCCAATGAATTTGAAAAGTACCACTTGTTTGTTGGGGACCTGGACAAAGTGGTCAACCTGTTGCTGTCACTCTCTGGACGACTGGCCCGGGTGGAGAATGCTCTGAACAGCATCGATTCAGAGGCCAACCAGGAGAAGTTGGTACTGATAGAGAAGAAGCAGCAGCTGACGGGGCAGTTGGCAGATGCCAAGGAGCTGAAGGAGCACGTGGACCGCCGGGAGAAGTTGGTGTTTGGCATGGTCTCCCGCTACCTGCCTCAGGACCAGCTCCAAGATTACCAGCACTTTGTCAAGATGAAATCTGCTCTCATCATTGAACAGCGAGAGCTGGAGGAGAAGATCAAGCTCGGGGAAGAGCAACTCAAATGTCTCAGGGAGAGTCTACTCCTGGGGCCCAGCAATTTCTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFMENRPGSFQYVPVQLQGGAPWGFTLKGGLEHCEPLTVSKIEDGGKAALSQKMRTGDELVNINGTPLYGSRQEALILIKGSFRILKLIVRRRNAPVSRPHSWHVAKLLEGCPEAATTMHFPSEAFSLSWHSGCNTSDVCVQWCPLSRHCSTEKSSSIGSMESLEQPGQATYESHLLPIDQNMYPNQRDSAYSSFSASSNASDCALSLRPEEPASTDCIMQGPGPTKAPSGRPNVAETSGGSRRTNGGHLTPSSQMSSRPQEGYQSGPAKAVRGPPQPPVRRDSLQASRAQLLNGEQRRASEPVVPLPQKEKLSLEPVLPARNPNRFCCLSGHDQVTSEGHQNCEFSQPPESSQQGSEHLLMQASTKAVGSPKACDRASSVDSNPLNEASAELAKASFGRPPHLIGPTGHRHSAPEQLLASHLQHVHLDTRGSKGMELPPVQDGHQWTLSPLHSSHKGKKSPCPPTGGTHDQSSKERKTRQVDDRSLVLGHQSQSSPPHGEADGHPSEKGFLDPNRTSRAASELANQQPSASGSLVQQATDCSSTTKAASGTEAGEEGDSEPKECSRMGGRRSGGTRGRSIQNRRKSERFATNLRNEIQRRKAQLQKSKGPLSQLCDTKEPVEETQEPPESPPLTASNTSLLSSCKKPPSPRDKLFNKSMMLRARSSECLSQAPESHESRTGLEGRISPGQRPGQSSLGLNTWWKAPDPSSSDPEKAHAHCGVRGGHWRWSPEHNSQPLVAAAMEGPSNPGDNKELKASTAQAGEDAILLPFADRRKFFEESSKSLSTSHLPGLTTHSNKTFTQRPKPIDQNFQPMSSSCRELRRHPMDQSYHSADQPYHATDQSYHSMSPLQSETPTYSECFASKGLENSMCCKPLHCGDFDYHRTCSYSCSVQGALVHDPCIYCSGEICPALLKRNMMPNCYNCRCHHHQCIRCSVCYHNPQHSALEDSSLAPGNTWKPRKLTVQEFPGDKWNPITGNRKTSQSGREMAHSKTSFSWATPFHPCLENPALDLSSYRAISSLDLLGDFKHALKKSEETSVYEEGSSLASMPHPLRSRAFSESHISLAPQSTRAWGQHRRELFSKGDETQLDLLGARKKAFPPPRPPPPNWEKYRLFRAAQQQKQQQQQQQQQQKQQEEEEEEEEEEEEEEEEEEEEEAEEEEEELPPQYFSSETSGSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAEQESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSPPSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIESISRKLSVLREAQRGLLEDINANSALGEEVEANLKAVCKSNEFEKYHLFVGDLDKVVNLLLSLSGRLARVENALNSIDSEANQEKLVLIEKKQQLTGQLADAKELKEHVDRREKLVFGMVSRYLPQDQLQDYQHFVKMKSALIIEQRELEEKIKLGEEQLKCLRESLLLGPSNF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU59318
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Dear Simone, can you please mutate the DU59055 (S1089L). Thank you!
• Low Temp: No