cDNA Clone GFP (plant opt)

• Synonyms: HRCA1, RCA11, pVHL, VHL
• Plasmid: pBABED P 3FLAG VHL GFPp 1-9
• Parent Plasmid: pBABED P 3FLAG
• Expressed: 3FLAG-VHL-5GLY-GFPp 1-9
• Tag: FLAG

Sequence of Insert

VHL
GGATCCGCCATGCCCCGGAGGGCGGAGAACTGGGACGAGGCCGAGGTAGGCGCGGAGGAGGCAGGCGTCGAAGAGTACGGCCCTGAAGAAGACGGCGGGGAGGAGTCGGGCGCCGAGGAGTCCGGCCCGGAAGAGTCCGGCCCGGAGGAACTGGGCGCCGAGGAGGAGATGGAGGCCGGTCGGCCGCGGCCCGTGCTGCGCTCGGTGAACTCGCGCGAGCCCTCCCAGGTCATCTTCTGCAATCGCAGTCCGCGCGTCGTGCTGCCCGTATGGCTCAACTTCGACGGCGAGCCGCAGCCCTACCCAACGCTGCCGCCTGGCACGGGCCGCCGCATCCACAGCTACCGAGGTCACCTTTGGCTCTTCAGAGATGCAGGGACACACGATGGGCTTCTGGTTAACCAAACTGAATTATTTGTGCCATCTCTCAATGTTGACGGACAGCCTATTTTTGCCAATATCACACTGCCAGTGTATACTCTGAAAGAGCGATGCCTCCAGGTTGTCCGGAGCCTAGTCAAGCCTGAGAATTACAGGAGACTGGACATCGTCAGGTCGCTCTACGAAGATCTGGAAGACCACCCAAATGTGCAGAAAGACCTGGAGCGGCTGACACAGGAGCGCATTGCACATCAACGGATGGGAGATGGTGGAGGCGGAGGT

GFPp 1-9
GAATTCGCCATGCGCAAAGGCGAAGAACTGTTTACCGGCGTGGTGCCGATTCTGATTGAACTGGATGGCGATGTGAACGGCCATAAATTTTTTGTGCGCGGCGAAGGCGAAGGCGATGCGACCATTGGCAAACTGAGCCTGAAATTTATTTGCACCACCGGCAAACTGCCGGTGCCGTGGCCGACCCTGGTGACCACCCTGACCTATGGCGTGCAGTGCTTTAGCCGCTATCCGGATCACATGAAACGCCATGATTTTTTTAAAAGCGCGATGCCGGAAGGCTATGTGCAGGAACGCACCATTTATTTTAAAGATGATGGCACCTATAAAACCCGCGCGGAAGTGAAATTTGAAGGCGATACCCTGGTGAACCGCATTGAACTGAAAGGCATTGATTTTAAAGAAGATGGCAACATTCTGGGCCATAAACTGGAATATAACTTTAACAGCCATAAAGTGTATATTACCGCGGATAAACAGAACAACGGCATTAAAGCGAACTTTACCATTCGCCATAACGTGGAAGATGGCAGCGTGCAGCTGGCGGATCATTATCAGCAGAACACCCCGATTGGCGATGGCCCGGTGCTGCTGCCGTAAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSDYKDDDDKDYKDDDDKGSAMPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSRE
PSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLV
KPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGDGGGGGEFAMRKGEELFTGVVPILIELDGDVNGHKFFVRGEGEGDATIGKLSLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIYFKDDGTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHK
VYITADKQNNGIKANFTIRHNVEDGSVQLADHYQQNTPIGDGPVLLP*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pBABED P 3FLAG VHL GFPp 1-9123.16 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU60535
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Plant optimised GFP fragment for use in the construction of tripartite reporter and degron systems.
• Low Temp: No