cDNA Clone SPT6 (SUPT6H)

• Synonyms: SUPT6H; SPT6H
• Plasmid: pcDNA5D FRT/TO GFP SPT6
• Parent Plasmid: pcDNA5D FRT/TO BirA R118G MCS HA
• Expressed: GFP SPT6
• Genbank: NM_003170.4 (Q7KZ85)
• Tag: GFP
• Species: Human

Sequence of Insert

aCTAGTATGTCTGATTTTGTGGAAAGCGAGGCTGAGGAGTCAGAGGAAGAATACAATGATGAAGGCGAGGTGGTACCCCGAGTCACCAAGAAATTTGTGGAAGAGGAGGATGATGATGAGGAGGAGGAGGAGGAGAACCTAGATGATCAGGATGAGCAAGGCAACTTGAAAGGCTTTATCAATGACGATGATGATGAAGATGAAGGGGAGGAGGATGAGGGCAGTGACTCTGGTGATTCAGAAGATGATGTTGGCCACAAGAAGAGAAAACGCACCTCTTTTGATGACCGCCTGGAGGATGATGATTTTGACCTCATTGAGGAGAATTTGGGTGTCAAAGTCAAAAGAGGACAAAAGTACCGGCGTGTCAAAAAAATGTCAGATGACGAGGACGATGACGAGGAGGAATATGGCAAGGAGGAACATGAAAAAGAAGCTATTGCGGAAGAAATCTTCCAGGATGGGGAAGGGGAAGAAGGGCAGGAGGCCATGGAGGCCCCCATGGCTCCTCCAGAGGAGGAGGAAGAAGATGATGAGGAGTCAGATATTGACGACTTCATTGTGGATGATGATGGACAGCCTCTGAAAAAACCTAAGTGGCGGAAAAAGCTTCCTGGATACACAGACGCGGCCCTGCAAGAAGCCCAGGAAATCTTCGGTGTGGACTTTGACTATGATGAATTTGAGAAATACAATGAGTATGATGAAGAACTGGAGGAAGAGTATGAGTATGAGGATGATGAGGCTGAGGGTGAAATCCGAGTGCGCCCCAAGAAGACCACCAAGAAGCGTGTGAGCCGTAGGAGCATCTTTGAAATGTATGAGCCCAGTGAGCTAGAAAGCAGCCACCTCACAGATCAGGACAATGAAATCCGAGCCACTGACCTGCCTGAGAGGTTCCAGCTCCGCTCCATCCCAGTCAAGGGGGCTGAAGATGATGAACTAGAAGAAGAAGCTGACTGGATCTACAGGAATGCTTTTGCCACACCAACCATTTCTCTCCAGGAAAGCTGTGATTACCTAGACCGAGGGCAGCCAGCCAGCAGCTTCAGTCGGAAAGGGCCCAGCACAATTCAGAAGATCAAAGAGGCCCTGGGCTTCATGCGAAATCAGCATTTTGAGGTGCCTTTTATTGCCTTCTATCGAAAGGAGTATGTGGAGCCTGAGTTGCACATCAATGACCTATGGAGAGTCTGGCAGTGGGATGAAAAGTGGACCCAGCTGCGGATCCGTAAAGAGAACCTAACACGGCTGTTTGAGAAGATGCAGGCTTATCAGTATGAACAGATCTCTGCTGACCCTGACAAACCTCTTGCTGATGGCATCCGGGCTCTGGACACCACTGACATGGAGAGGCTCAAGGATGTCCAATCAATGGATGAGCTGAAAGATGTCTACAACCATTTTCTTCTTTATTATGGCCGAGACATCCCTAAGATGCAGAACGCCGCCAAAGCTAGCCGCAAGAAGCTGAAGCGTGTCAGGGAAGAGGGAGATGAAGAAGGTGAAGGTGACGAGGCAGAAGATGAGGAGCAGAGGGGGCCTGAGCTCAAGCAAGCCTCTCGCCGAGACATGTACACCATCTGCCAGAGTGCTGGGCTAGATGGCCTGGCCAAAAAGTTTGGGCTTACTCCCGAGCAGTTTGGGGAGAACCTGCGGGATAGCTACCAGCGGCACGAGACAGAGCAGTTTCCCGCGGAGCCCTTGGAGCTGGCCAAGGATTACGTTTGCAGCCAGTTCCCTACTCCAGAAGCTGTGCTAGAAGGCGCCCGCTACATGGTAGCCCTGCAGATTGCCCGTGAGCCCCTTGTCCGGCAGGTGCTGAGGCAAACCTTCCAAGAGAGAGCCAAGTTAAATATAACCCCCACCAAGAAAGGTAGAAAGGATGTGGATGAGGCCCACTATGCCTATTCCTTCAAGTATTTAAAGAACAAGCCTGTTAAGGAACTGAGAGATGACCAGTTTCTCAAGATATGCCTGGCTGAAGACGAAGGGCTCCTCACCACTGACATCAGCATAGATTTGAAGGGAGTGGAAGGCTATGGCAACGACCAGACATATTTTGAGGAGATAAAACAGTTTTACTACCGAGATGAGTTCAGCCACCAGGTGCAGGAGTGGAACCGGCAGCGCACCATGGCCATCGAACGGGCTTTACAGCAGTTCCTCTATGTGCAGATGGCCAAAGAACTCAAGAACAAGCTGCTGGCTGAAGCCAAGGAATATGTCATAAAGGCCTGTAGTCGAAAGCTCTACAATTGGTTGAGAGTGGCACCCTACCGACCAGATCAGCAGGTGGAAGAAGATGACGACTTTATGGACGAGAACCAAGGGAAGGGCATTCGAGTCCTCGGCATTGCTTTCTCCTCTGCCAGAGATCACCCTGTGTTCTGCGCCCTGGTCAATGGTGAAGGAGAAGTGACAGACTTCCTTCGACTGCCCCATTTTACCAAACGGCGAACTGCATGGAGAGAGGAAGAGCGGGAAAAGAAGGCTCAAGACATTGAAACGCTAAAGAAATTTCTCCTGAATAAGAAGCCTCATGTAGTGACAGTTGCAGGAGAGAACAGGGACGCCCAGATGTTGATTGAAGATGTGAAGCGCATTGTACATGAGCTGGACCAGGGCCAGCAGCTGTCATCTATTGGGGTAGAGCTGGTTGACAACGAGTTGGCCATTCTCTATATGAACAGCAAGAAGTCAGAGGCAGAGTTCCGGGATTATCCTCCAGTGCTGAGACAGGCCGTCTCCCTGGCCCGGCGCATCCAGGACCCTCTGATTGAATTTGCCCAGGTGTGCAGTTCCGATGAAGACATCCTGTGTCTCAAGTTTCACCCCTTGCAGGAGCATGTGGTGAAAGAGGAGCTGCTCAACGCCTTGTACTGTGAATTTATCAACCGAGTCAATGAGGTCGGGGTCGATGTCAACCGTGCCATTGCCCACCCTTACAGCCAGGCCTTGATCCAGTATGTTTGTGGCCTGGGACCTCGGAAAGGGACCCACCTCCTGAAGATCCTGAAGCAGAACAACACCCGGCTCGAGAGCCGGACCCAGCTGGTCACCATGTGCCACATGGGTCCCAAAGTCTTCATGAATTGTGCTGGCTTCCTCAAGATCGACACGGCCTCCCTGGGGGACAGCACTGACTCATATATTGAAGTCCTTGATGGTTCCCGTGTCCACCCTGAGACTTATGAGTGGGCTAGGAAGATGGCAGTGGATGCCCTGGAATACGATGAATCAGCCGAGGATGCCAATCCTGCAGGAGCCCTTGAAGAAATCTTGGAAAACCCAGAGCGACTGAAAGACCTGGACCTTGATGCCTTTGCAGAAGAGCTGGAGAGGCAGGGCTATGGTGACAAACACATCACACTCTATGACATCCGGGCAGAGCTGAGCTGTCGATATAAGGACCTCCGGACAGCCTACCGCTCTCCCAACACAGAGGAGATCTTCAATATGTTAACCAAAGAAACACCAGAGACCTTCTACATTGGAAAGCTCATCATCTGCAATGTCACTGGCATTGCCCACAGGCGTCCCCAGGGTGAGAGCTATGACCAGGCGATCCGCAATGATGAGACAGGGCTGTGGCAGTGCCCCTTCTGTCAGCAGGACAATTTCCCTGAACTAAGCGAGGTGTGGAACCACTTTGACAGCGGTTCGTGCCCAGGCCAGGCCATCGGTGTCAAAACACGGCTAGACAATGGTGTCACCGGCTTCATCCCCACCAAATTCCTCAGTGACAAAGTGGTAAAGCGGCCAGAAGAACGAGTGAAGGTGGGAATGACTGTTCACTGCCGCATCATGAAGATTGACATTGAGAAGTTCAGTGCAGACCTGACCTGCCGCACCTCAGACCTCATGGACAGGAACAATGAGTGGAAGCTGCCCAAAGACACCTACTATGACTTTGATGCTGAAGCTGCAGACCACAAGCAGGAGGAGGACATGAAGCGGAAGCAGCAGCGGACCACATACATCAAGAGAGTGATCGCACACCCATCCTTCCATAATATCAATTTCAAGCAAGCAGAAAAGATGATGGAGACCATGGACCAGGGTGATGTGATTATCCGACCAAGCAGCAAGGGCGAGAACCACCTGACAGTGACCTGGAAAGTCAGTGATGGCATCTACCAGCATGTGGATGTGCGGGAGGAGGGCAAGGAAAATGCCTTCAGCCTGGGAGCCACTCTGTGGATCAACAGTGAGGAATTCGAAGATTTGGATGAGATTGTTGCTCGCTATGTCCAGCCCATGGCATCCTTTGCCCGGGACCTTCTGAATCACAAGTATTATCAGGACTGCAGCGGTGGGGACCGCAAGAAATTAGAGGAGCTGCTCATCAAAACTAAGAAGGAGAAGCCCACCTTCATCCCTTATTTCATCTGTGCCTGCAAGGAACTGCCCGGCAAGTTCCTACTGGGATACCAGCCCCGGGGTAAACCCAGGATAGAATATGTAACGGTGACTCCAGAGGGATTCCGGTACCGGGGCCAGATCTTCCCAACCGTGAATGGACTGTTTAGATGGTTTAAGGATCACTACCAGGATCCTGTACCAGGCATCACCCCTAGCAGCAGCAGCAGGACCCGGACACCTGCCTCTATCAATGCTACCCCAGCCAACATCAACCTTGCAGATCTGACACGGGCTGTGAATGCCCTGCCTCAGAACATGACTTCACAGATGTTCAGTGCCATTGCTGCGGTGACAGGCCAAGGACAGAACCCTAATGCCACCCCAGCCCAGTGGGCCTCCAGCCAGTACGGCTATGGCGGCAGTGGAGGCGGCAGCAGTGCTTACCACGTATTCCCAACGCCAGCCCAGCAGCCAGTGGCCACACCACTAATGACCCCTAGCTACTCCTACACGACCCCAAGCCAGCCCATCACCACCCCTCAGTACCACCAGCTCCAGGCCAGCACCACCCCACAGTCGGCCCAGGCCCAGCCCCAGCCCTCTTCCAGCTCCCGGCAACGGCAGCAGCAGCCAAAGTCCAACAGCCATGCAGCCATCGACTGGGGAAAAATGGCGGAGCAGTGGCTGCAGGAAAAGGAGGCAGAACGGCGGAAACAGAAGCAGCGGCTGACACCTCGGCCCTCCCCCAGCCCCATGATCGAAAGCACCCCCATGTCCATTGCTGGCGATGCCACCCCACTCCTGGACGAGATGGATCGGTAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSPGEFASMSDFVESEAEESEEEYNDEGEVVPRVTKKFVEEEDDDEEEEEENLDDQDE
QGNLKGFINDDDDEDEGEEDEGSDSGDSEDDVGHKKRKRTSFDDRLEDDDFDLIEENLGVKVKRGQKYRRVKKMSDDEDDDEEEYGKEEHEKEAIAEEIF
QDGEGEEGQEAMEAPMAPPEEEEEDDEESDIDDFIVDDDGQPLKKPKWRKKLPGYTDAALQEAQEIFGVDFDYDEFEKYNEYDEELEEEYEYEDDEAEGE
IRVRPKKTTKKRVSRRSIFEMYEPSELESSHLTDQDNEIRATDLPERFQLRSIPVKGAEDDELEEEADWIYRNAFATPTISLQESCDYLDRGQPASSFSR
KGPSTIQKIKEALGFMRNQHFEVPFIAFYRKEYVEPELHINDLWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMER
LKDVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRGPELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENL
RDSYQRHETEQFPAEPLELAKDYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEAHYAYSFKYLKNKPVKELRD
DQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQFYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLY
NWLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRLPHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTV
AGENRDAQMLIEDVKRIVHELDQGQQLSSIGVELVDNELAILYMNSKKSEAEFRDYPPVLRQAVSLARRIQDPLIEFAQVCSSDEDILCLKFHPLQEHVV
KEELLNALYCEFINRVNEVGVDVNRAIAHPYSQALIQYVCGLGPRKGTHLLKILKQNNTRLESRTQLVTMCHMGPKVFMNCAGFLKIDTASLGDSTDSYI
EVLDGSRVHPETYEWARKMAVDALEYDESAEDANPAGALEEILENPERLKDLDLDAFAEELERQGYGDKHITLYDIRAELSCRYKDLRTAYRSPNTEEIF
NMLTKETPETFYIGKLIICNVTGIAHRRPQGESYDQAIRNDETGLWQCPFCQQDNFPELSEVWNHFDSGSCPGQAIGVKTRLDNGVTGFIPTKFLSDKVV
KRPEERVKVGMTVHCRIMKIDIEKFSADLTCRTSDLMDRNNEWKLPKDTYYDFDAEAADHKQEEDMKRKQQRTTYIKRVIAHPSFHNINFKQAEKMMETM
DQGDVIIRPSSKGENHLTVTWKVSDGIYQHVDVREEGKENAFSLGATLWINSEEFEDLDEIVARYVQPMASFARDLLNHKYYQDCSGGDRKKLEELLIKT
KKEKPTFIPYFICACKELPGKFLLGYQPRGKPRIEYVTVTPEGFRYRGQIFPTVNGLFRWFKDHYQDPVPGITPSSSSRTRTPASINATPANINLADLTR
AVNALPQNMTSQMFSAIAAVTGQGQNPNATPAQWASSQYGYGGSGGGSSAYHVFPTPAQQPVATPLMTPSYSYTTPSQPITTPQYHQLQASTTPQSAQAQ
PQPSSSSRQRQQQPKSNSHAAIDWGKMAEQWLQEKEAERRKQKQRLTPRPSPSPMIESTPMSIAGDATPLLDEMDR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63296
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Low Temp: No