cDNA Clone SLX4 (yeast)

• Plasmid: pRS413 PRO-13Myc-ySLX4 T72A T113A S289A T319A S329A S355A
• Parent Plasmid: pRS413
• Expressed: 13Myc-ySLX4 T72A T113A S289A T319A S329A S355A
• Genbank: Z73307.1 GI:1360550
• Tag: Myc
• Species: Yeast

Sequence of Insert

GCGGCCGCTCTAGAACTAGTGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTGCGTCGACTTTCTGTCCTGTCTTCCAGGTTAAAGATT
AGCTTCTAATATTTTAGGTGGTTTATTATTTAATTTTATGCTGATTAATTTATTTACTTTCGTATTCGGTTTTGTACCTTTAGCTATGATCTTAGCTAAT
TGAAGAGGGTGGTGTGATCTTTAACCATACCTTATTATCTTTCAGCTGCTTACCATTTTCTTATATTGATTTTTAGCGAAAGATTTTTATTCACAAGCTT
TTTTTATCCTTAATGCTCGAATACTACAACAAAACAAAAAACATTAAACAGTTTTTAATTTTGTGAACAAACTGAATTACAAGGCCTTACATCTTATTTA
GAATATATTAAGAAACAGAGGCCAACATGCCTTCTTAATTATATTGATATGGACCTCTGTCCTTCCTAAAAACGGGTTTTTGTTCGATGAAAAATCACCA
GTAGAGCACCATATATGAATTTACAATCATTGTAGGGAAAAGAAAACTTGTTCTGCTTCGCCAATTGATTTCATTTCTTTTTTTCCTTTGTTTTTGTTGT
ATACTATTAATATGCGTAGTTCTTTGTCACTTAGTGGTTTTTTGTTTAATTTATAACGCGAAAGCTGCATACTAAAAATATAGAGATGAATGGAACTCCG
AACAAGCAAGAGAGATATTATCTATCTCTGTTCCATAATAATAACCAGTAGTTCAGTTGGGGAACTTAACCATGGCAGGTCGACGGATCCCCGGGTTAAT
TAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTG
ATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGAAGACTTGAACGGACTCG
ACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAAAAATTAATCTCAGAAGA
AGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAGACTTGAACGGTGAACAA
AAATTAATCTCAGAAGAAGACTTGAACGGACTCGACGGTGAACAAAAGTTGATTTCTGAAGAAGATTTGAACGGTGAACAAAAGCTAATCTCCGAGGAAG
ACTTGAACGGTGAACAAAAATTAATCAATCACACCATGGAACTTCAGAGGGCACAACGGAATTTGAAGTTTTTACAAAACGAGGATTATGTGAACGTTAC
CGATCAAACTAACCTGAATGGAGAGAGCCAGAATGCTTACTCTTTAGGAATGGAAACTCAAGTACCGGAAATGCAGTTTTCCTTATCATCTGATGATGAT
TCAATAGGTACGCAAGTAAAAAGCGTAACTGCACAAAAATCGCCTATGGCGCAAGAGACTACGAAAAACGATACAGAAAGAAATAAAGATGTTGATAAAA
GTTGTAACCCAGTCAGCACTTCCCATCCCGATTTAGGCGGATCAAATATCGAGGAGAACATATTCATTAACGCTCAAATTCAAAGCCGACTTGATGACGC
AGAGGAAGAAACAAATCTTAAGTTAAAACTTGAAAAATTCAAGTATTCGTTCAAGAGTAGTAACGCAGATGATACCCACAGCAATGCAAATGTAACAGCT
AAAAGAAGACCAGCAATAAGGAAGGCTAATTCGAAACTCAAAACAAAACCTAAAACTAAACGAGACCCTAAAATTATCAAAAATATAACTGATTTCAATA
TTAATAACTACGAACGTTCAAGGACTGCCAGCTTATTAAAGCAATTATCAGGTAAGCACAAGAAGGTTTTGGACATAATAAAGACGCAAAATGAAGGAAA
CAGTGATAAACCTCCCAGAGCAAGGAATAACAAGGGTGAAAAAGCTACTTTTGATACATATAGTGAACAGGAGTGGAAAGATATTATGAAACTTCTTCTA
CAAAAGTTTCCGCAAAGTGAGGAAACGGATTTGAATGAAGTCCAGAAATTTTTATATGGAAGCGAAAAAAGCTCGAATTCTCTAGACAATCAAGAATCCG
CACAACAAAGACTTTGGACAGCATCACAATTACCACCCGAGCTTCCCGATGAGGCTATTCAACCAGAGCAGGAAGAAAGGATACGCGATGCCCAAAGTGC
AGTAAATTTCTTATCGTTGGCTCAAGTAATGGATGACAAAAGTGAAATTATGAAAGACGAAGAAAGCATAATTATTTCACGGGGAGATTCTACAAGCGCT
CAAGAATATGGAAACGGGTTAGAGCCACAACAACCGGTAGGAAATGTTGTTGGTGAAGATATTGAACTGGCTGTGGGAACACGTATAAATGCATTTTCAC
TGACAGATTATAAAGCTTGCAAACCTATGTCTGTTGAGGTAAGTAGAAGATGCGAAAACAGTACGGATAATGATTATGACAATATTTCTATTGTCTCAGA
TACTACAGATGAGACATCCACATTGTTTCCACTAGACCAGTATCGGTATGTTTTCATTGAAAATGATGAGAGACCACCATTAGCCACCGACACGATAGGC
AGCACACAGTTTTTCACTCCAAACACATCGCCTTTGGATGGGATAATCGATTTAACACAGGAATCATTCAAAGCTGTTCGATCGTTAATATCACCTTTGA
AAGTAGAAAACAACAAAACAGGGGTAACATCACAAGCATCAAATCAGGTTCAGGTACCAGCTACAAGAACCCCTACAATTATTCCTCAAAAGAACCTTAC
TACAACTTTAAAAACTGAAGAAGAAAAGAATAACATAGGAAGTTCCATCAGAGTTAAATTATTGCAAGAAAGCGTAGTTAAGCTAAACCCCAAACTGGTG
AAACATAACTTTTATAGAGTTGAGGCAAATGATTCTGAAGAAGAGGAGACAGAATTTGATGACCAATTCTGCATTGCTGATATCCAATTGGTAGATTCCT
CCAAAATTTCGACTAAAGATTCTACACAAAATCCTACTACTAGCAACGACATTATTGATACCTCTGCAGCTAGTTCCATAGCTTCTCCCGAGAAATTTTG
TGAGATCATGATGTCCCAATCAATGAAAGAGCTTCGACAAAGCTTGAAAACAGTGGGGTTGAAACCTATGAGGACGAAGGTTGAAATCATTCAATCCTTA
CAAACTGCATCTCAAATTCTGTCCACAGCTAATCCAGATAATAAAGGTGAGCATGGTGGGGTGGCAAATTTCAGTAAGATAGAAATATTTGATCATTTAA
CCGAACTAATTGAAGCCTTCCCTGATTTTTTGGAGCGAATATATACCTTTGAACCCATTCCGCTAAATGAGCTAATTGAAAAGCTGTTCAGCGCCGAGCC
ATTTGTTTCGCAAATCGACGAAATGACCATCAGAGAATGGGCCGATGTTCAAGGAATATGTCTGAGAAACGATAAAAAGTAGGGATCCCCCGGGCTGCAG
GAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAG

Amino Acid Sequence

MAGRRIPGLINGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDL
NGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLINHTMELQRAQRNLKF
LQNEDYVNVTDQTNLNGESQNAYSLGMETQVPEMQFSLSSDDDSIGTQVKSVTAQKSPMAQETTKNDTERNKDVDKSCNPVSTSHPDLGGSNIEENIFIN
AQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNANVTAKRRPAIRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVL
DIIKTQNEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGSEKSSNSLDNQESAQQRLWTASQLPPELPDEAIQPEQ
EERIRDAQSAVNFLSLAQVMDDKSEIMKDEESIIISRGDSTSAQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDYKACKPMSVEVSRRCENSTDN
DYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLATDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVPATRT
PTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEANDSEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAA
SSIASPEKFCEIMMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKIEIFDHLTELIEAFPDFLERIYTFEPIPLNE
LIEKLFSAEPFVSQIDEMTIREWADVQGICLRNDKK*

• Vector Type: Other
• Antibiotic: Amp
• Full Snapgene Map: pRS413 PRO-13Myc-ySLX4 T72A T113A S289A T319A S329A S355A416.85 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU11034
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: This plasmid identical to DU11135.; ; All BTBD12 (SLX4) plasmids MUST be grown at 30 degrees C or less to prevent recombination.
• Low Temp: No