cDNA Clone DVL1

• Plasmid: pcDNA5frtTO MYC DU DVL1 isoform 1
• Parent Plasmid: pcDNA5frtTO MYC DU
• Expressed: myc-DVL1 isoform 1
• Genbank: NM_001330311.1 → NP_001317240.1
• Tag: Myc
• Species: Human

Sequence of Insert

GGATCCATGGGCGAGACCAAGATTATCTACCACATGGACGAGGAGGAGAC
GCCGTACCTGGTCAAGCTGCCCGTGGCCCCCGAGCGCGTCACGCTGGCCG
ACTTCAAGAACGTGCTCAGCAACCGGCCCGTGCACGCCTACAAATTCTTC
TTTAAGTCCATGGACCAGGACTTCGGGGTGGTGAAGGAGGAGATCTTTGA
TGACAATGCCAAGCTTCCCTGCTTCAACGGCCGCGTGGTCTCCTGGCTGG
TCCTGGCTGAGGGTGCTCACTCGGATGCGGGGTCCCAGGGCACGGACAGC
CACACAGACCTGCCCCCGCCTCTTGAGCGGACAGGCGGCATCGGGGACTC
CCGGCCCCCCTCCTTCCACCCGAATGTGGCCAGCAGCCGTGACGGGATGG
ACAACGAGACAGGCACGGAGTCCATGGTCAGTCACCGGCGGGAGCGTGCC
CGACGCCGGAACCGCGAGGAGGCCGCCCGGACCAATGGGCACCCAAGGGG
AGACCGACGGCGGGATGTGGGGCTGCCCCCAGACAGCGCGTCCACCGCCC
TCAGCAGCGAGCTTGAGTCCAGCAGCTTTGTGGACTCGGACGAGGATGGC
AGCACGAGCAGGCTCAGCAGCTCCACGGAGCAGAGCACCTCATCCAGACT
CATCCGGAAGCACAAACGCCGGCGGAGGAAGCAGCGCCTTCGGCAGGCGG
ACCGGGCCTCCTCCTTCAGCAGCATAACCGACTCCACCATGTCCCTCAAC
ATCGTCACTGTCACGCTCAACATGGAAAGACATCACTTTCTGGGCATCAG
CATCGTGGGGCAGAGCAACGACCGTGGAGACGGCGGCATCTACATTGGCT
CCATCATGAAGGGCGGGGCTGTGGCCGCTGACGGCCGCATCGAGCCCGGC
GACATGTTGCTGCAGGTGAATGACGTGAACTTTGAGAACATGAGCAATGA
CGATGCCGTGCGGGTGCTGCGGGAGATCGTTTCCCAGACGGGGCCCATCA
GCCTCACTGTGGCCAAGTGCTGGGACCCAACGCCCCGAAGCTACTTCACC
GTCCCACGGGCTGACCCGGTGCGGCCCATCGACCCCGCCGCCTGGCTGTC
CCACACGGCGGCACTGACAGGAGCCCTGCCCCGCTACGGTACGAGTCCCT
GCTCCAGCGCCGTCACGCGCACCAGCTCCTCCTCACTAACCAGCTCCGTG
CCTGGTGCTCCACAGCTGGAAGAGGCGCCGCTGACGGTGAAGAGTGACAT
GAGCGCCGTCGTCCGGGTCATGCAGCTGCCAGACTCGGGACTGGAGATCC
GCGACCGCATGTGGCTCAAGATCACCATCGCCAATGCCGTCATCGGGGCG
GACGTGGTGGACTGGCTGTACACACACGTGGAGGGCTTCAAGGAGCGGCG
GGAGGCCCGGAAGTACGCCAGCAGCTTGCTGAAGCACGGCTTCCTGCGGC
ACACGGTCAACAAGATCACCTTCTCCGAGCAGTGCTACTACGTCTTCGGG
GATCTCTGCAGCAATCTCGCCACCCTGAACCTCAACAGTGGCTCCAGTGG
GACTTCGGATCAGGACACGCTGGCCCCGCTGCCCCACCCGGCTGCCCCCT
GGCCTCTGGGTCAGGGCTACCCCTACCAGTACCCGGGACCCCCACCCTGC
TTCCCGCCTGCCTACCAGGACCCGGGCTTTAGCTATGGCAGCGGCAGCAC
CGGGAGTCAGCAGAGTGAAGGGAGCAAAAGCAGTGGGTCCACCCGGAGCA
GCCGCCGGGCCCCGGGCCGTGAGAAGGAGCGTCGGGCGGCGGGAGCTGGG
GGCAGTGGCAGTGAATCGGATCACACGGCACCGAGTGGGGTGGGGAGCAG
CTGGCGAGAGCGTCCGGCCGGCCAGCTCAGCCGTGGCAGCAGCCCACGCA
GTCAGGCCTCAGCTACCGCCCCGGGGCTCCCCCCGCCCCACCCCACGACC
AAGGCCTATACAGTGGTGGGGGGGCCACCCGGGGGACCCCCTGTCCGGGA
GCTGGCTGCCGTCCCCCCGGAATTGACAGGCAGCCGCCAGTCCTTCCAGA
AGGCTATGGGGAACCCCTGCGAGTTCTTCGTGGACATCATGTGAGCGGCC
GC

Amino Acid Sequence

MEQKLISEEDLGSMGETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVL
SNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGA
HSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGT
ESMVSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELE
SSSFVDSDEDGSTSRLSSSTEQSTSSRLIRKHKRRRRKQRLRQADRASSF
SSITDSTMSLNIVTVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGG
AVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAK
CWDPTPRSYFTVPRADPVRPIDPAAWLSHTAALTGALPRYGTSPCSSAVT
RTSSSSLTSSVPGAPQLEEAPLTVKSDMSAVVRVMQLPDSGLEIRDRMWL
KITIANAVIGADVVDWLYTHVEGFKERREARKYASSLLKHGFLRHTVNKI
TFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAPWPLGQG
YPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPG
REKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASAT
APGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNP
CEFFVDIM*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29804
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Clone contains listed SNP Ala2Gly; plus silent changes at Pro122 (CCA > CCG), Ser635 (TCG > TCA); ; the following non-silent mutations (all derived from source); Ser564, Asn686 and Ile694 all restored
• Low Temp: No