cDNA Clone GFP

• Plasmid: pCMV5D GFP 1-10 no stop
• Parent Plasmid: pCMV5D

Sequence of Insert

GGATCCGCCATGGTCTCCAAAGGAGAAGAACTGTTTACTGGTGTTGTGCCAATTTTGGTTGAACTCGATGGTGATGTCAACGGACATAAGTTCTCAGTGAGAGGCGAAGGAGAAGGTGACGCCACCATTGGAAAATTGACTCTTAAATTCATCTGTACTACTGGTAAACTTCCTGTACCTTGGCCGACTCTCGTAACAACGCTTACGTACGGAGTTCAGTGCTTTTCGAGATACCCAGACCACATGAAAAGACATGACTTTTTTAAGTCGGCTATGCCTGAAGGTTACGTGCAAGAAAGAACAATTTCGTTCAAAGATGATGGAAAATATAAAACTAGAGCAGTTGTTAAATTTGAAGGAGATACTTTGGTTAACCGCATTGAACTGAAAGGAACAGATTTTAAAGAAGATGGTAATATTCTTGGACACAAACTCGAATACAATTTTAATAGTCATAACGTATACATCACTGCTGATAAGCAAAAGAACGGAATTAAAGCGAATTTCACAGTACGCCATAATGTAGAAGATGGCAGTGTTCAACTTGCCGACCATTACCAACAAAACACCCCTATTGGAGATGGTCCGGTACTTCTTCCTGATAATCACTACCTCTCAACACAAACAGTCCTGAGCAAAGATCCAAATGAAAAAGGCGCTGGAGCGGGTGCTGGAGCAGGTGCGCTCGAG

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIS
FKDDGKYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQTVLSKDPNEKGAGAGAGAGA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU64300
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Genes of interest may be added XhoI-NotI downstream of the 5xGA linker, to be used in conjunction with a plasmids expressing GFP11 tagged proteins (Tripartite).
• Low Temp: No