cDNA Clone DCAF9 (WDTC1)

• Synonyms: ADP; DCAF9
• Plasmid: pcDNA5D FRT/TO puro mScarlet WDTC1
• Parent Plasmid: pcDNA5D FRT/TO puro mScarlet
• Expressed: mScarlet WDTC1
• Genbank: NM_001276252.1 with delta E317*
• Tag: mScarlet
• Species: Human

Sequence of Insert

GGATCCATGGCGAAAGTCAACATAACTAGAGACCTCATCCGTAGGCAGATCAAGGAGCGGGGTGCCCTGAGCTTTGAGCGGCGCTACCATGTCACTGACCCCTTTATCCGGCGGCTGGGCCTGGAAGCAGAGCTGCAGGGTCACTCAGGATGTGTCAACTGTCTGGAGTGGAATGAGAAAGGAGACTTGCTGGCCTCTGGTTCCGATGACCAGCACACGATTGTGTGGGACCCGCTGCACCACAAGAAGCTGCTCTCCATGCACACGGGACACACCGCAAATATCTTCTCTGTCAAGTTCCTGCCTCACGCTGGGGACCGCATCTTGATCACGGGGGCAGCCGACTCTAAGGTGCATGTGCACGACCTGACAGTAAAGGAGACCATCCACATGTTTGGAGACCACACAAACCGGGTGAAGCGCATCGCCACAGCGCCCATGTGGCCCAACACATTCTGGAGTGCTGCTGAGGATGGGCTTATCCGCCAGTATGACCTTCGAGAGAACAGCAAACACTCGGAGGTGCTGATTGACCTGACAGAGTACTGTGGCCAGCTGGTGGAGGCCAAGTGCCTCACTGTCAACCCCCAGGACAACAACTGCCTGGCAGTTGGGGCCAGCGGGCCCTTCGTGAGGCTCTATGACATCCGCATGATCCATAACCACAGAAAGAGCATGAAGCAGAGCCCTTCAGCGGGTGTGCACACCTTCTGTGACCGGCAGAAACCCCTTCCGGACGGTGCAGCCCAGTATTACGTAGCAGGTCACCTGCCAGTGAAGCTTCCTGACTACAACAACCGTTTGAGAGTGCTGGTTGCCACCTATGTGACCTTCAGCCCCAATGGCACAGAGCTACTAGTCAACATGGGGGGGGAACAGGTCTATTTGTTTGACTTGACTTACAAGCAGCGGCCGTACACCTTCCTCTTGCCTAGAAAATGCCACTCCTCGGGGGTCCAGAATGGCAAGATGTCCACCAACGGTGTGTCCAACGGTGTGTCCAATGGCCTGCACCTTCATAGCAATGGCTTCCGGCTGCCGGAGAGTAGGGGACATGTCAGCCCCCAAGTAGAGCTACCACCATACCTGGAGCGTGTGAAACAGCAAGCCAATGAGGCTTTTGCCTGCCAGCAGTGGACCCAAGCCATTCAGCTTTACAGCAAGGCTGTGCAGAGGGCCCCTCACAATGCCATGCTTTATGGAAACCGAGCAGCAGCCTACATGAAGCGCAAGTGGGATGGTGACCACTATGATGCCCTGAGGGACTGCCTCAAGGCCATCTCCCTAAACCCATGCCACCTGAAGGCACACTTTCGCCTGGCCCGCTGCCTCTTTGAGCTCAAGTATGTGGCTGAAGCCCTGGAGTGCCTGGACGACTTCAAAGGGAAATTTCCGGAGCAGGCCCACAGCAGCGCTTGTGATGCATTGGGCCGCGACATCACAGCTGCCCTCTTCTCTAAAAATGATGGTGAGGAGAAGAAGGGACCTGGTGGCGGCGCCCCAGTCCGCCTCCGCAGCACGAGCCGCAAGGACTCCATCTCAGAGGATGAAATGGTGCTGCGGGAGCGAAGCTACGACTATCAGTTCCGCTACTGCGGCCACTGCAACACCACCACGGATATCAAAGAGGCCAATTTCTTTGGCAGCAACGCTCAGTATATCGTCAGTGGCTCTGACGATGGCTCCTTCTTCATCTGGGAAAAGGAGACCACCAACCTGGTCCGTGTGCTCCAAGGGGATGAGTCCATTGTCAACTGCCTGCAGCCACACCCCAGCTACTGCTTCCTGGCCACCAGTGGCATCGATCCTGTTGTGCGGCTCTGGAACCCCCGACCAGAGAGTGAAGACCTCACAGGCCGAGTCGTGGAAGATATGGAGGGTGCTTCACAGGCCAACCAGCGGCGCATGAATGCAGACCCGTTGGAGGTGATGCTGCTCAACATGGGCTACCGGATCACGGGCCTGAGCAGTGGGGGTGCCGGGGCCTCTGATGATGAGGACAGCTCTGAGGGCCAGGTGCAGTGCCGGCCCAGCTAGGCGGCCGC

Amino Acid Sequence

MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNF
EDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKL
DITSHNEDYTVVEQYERSEGRHSTGGMDELYKGSMAKVNITRDLIRRQIKERGALSFERRYHVTDPFIRRLGLEAELQGHSGCVNCLEWNEKGDLLASGS
DDQHTIVWDPLHHKKLLSMHTGHTANIFSVKFLPHAGDRILITGAADSKVHVHDLTVKETIHMFGDHTNRVKRIATAPMWPNTFWSAAEDGLIRQYDLRE
NSKHSEVLIDLTEYCGQLVEAKCLTVNPQDNNCLAVGASGPFVRLYDIRMIHNHRKSMKQSPSAGVHTFCDRQKPLPDGAAQYYVAGHLPVKLPDYNNRL
RVLVATYVTFSPNGTELLVNMGGEQVYLFDLTYKQRPYTFLLPRKCHSSGVQNGKMSTNGVSNGVSNGLHLHSNGFRLPESRGHVSPQVELPPYLERVKQ
QANEAFACQQWTQAIQLYSKAVQRAPHNAMLYGNRAAAYMKRKWDGDHYDALRDCLKAISLNPCHLKAHFRLARCLFELKYVAEALECLDDFKGKFPEQA
HSSACDALGRDITAALFSKNDGEEKKGPGGGAPVRLRSTSRKDSISEDEMVLRERSYDYQFRYCGHCNTTTDIKEANFFGSNAQYIVSGSDDGSFFIWEK
ETTNLVRVLQGDESIVNCLQPHPSYCFLATSGIDPVVRLWNPRPESEDLTGRVVEDMEGASQANQRRMNADPLEVMLLNMGYRITGLSSGGAGASDDEDS
SEGQVQCRPS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU63930
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: delta E317 *As seen in other published sequences for this protein.
• Low Temp: No