cDNA Clone VRK2

• Synonyms: serine/threonine-protein kinase VRK2 isoform 1
• Plasmid: pBabeD puro GFP-VRK2
• Parent Plasmid: pBabeD puro GFP
• Expressed: GFP-VRK2
• Genbank: NM_006296.6, NP_006287.2
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGCCACCAAAAAGAAATGAAAAATACAAACTTCCTATTCCATT
TCCAGAAGGCAAGGTTCTGGATGATATGGAAGGCAATCAGTGGGTACTGG
GCAAGAAGATTGGCTCTGGAGGATTTGGATTGATATATTTAGCTTTCCCC
ACAAATAAACCAGAGAAAGATGCAAGACATGTAGTAAAAGTGGAATATCA
AGAAAATGGCCCGTTATTTTCAGAACTTAAATTTTATCAGAGAGTTGCAA
AAAAAGACTGTATCAAAAAGTGGATAGAACGCAAACAACTTGATTATTTA
GGAATTCCTCTGTTTTATGGATCTGGTCTGACTGAATTCAAGGGAAGAAG
TTACAGATTTATGGTAATGGAAAGACTAGGAATAGATTTACAGAAGATCT
CAGGCCAGAATGGTACCTTTAAAAAGTCAACTGTCCTGCAATTAGGTATC
CGAATGTTGGATGTACTGGAATATATACATGAAAATGAATATGTTCATGG
TGATATAAAAGCAGCAAATCTACTTTTGGGTTACAAAAATCCAGACCAGG
TTTATCTTGCAGATTATGGACTTTCCTACAGATATTGTCCCAATGGGAAC
CACAAACAGTATCAGGAAAATCCTAGAAAAGGCCATAATGGGACAATAGA
GTTTACCAGCTTGGATGCCCACAAGGGAGTAGCCTTGTCCAGACGAAGTG
ACGTTGAGATCCTCGGCTACTGCATGCTGCGGTGGTTGTGTGGGAAACTT
CCCTGGGAACAGAACCTGAAGGACCCTGTGGCTGTGCAGACTGCTAAAAC
AAATCTGTTGGACGAGCTCCCCCAGTCAGTGCTTAAATGGGCTCCTTCTG
GAAGCAGTTGCTGTGAAATAGCCCAATTTTTGGTATGTGCTCATAGTTTA
GCATATGATGAAAAGCCAAACTATCAAGCCCTCAAGAAAATTTTGAACCC
TCATGGAATACCTTTAGGACCACTGGACTTTTCCACAAAAGGACAGAGTA
TAAATGTCCATACTCCAAACAGTCAAAAAGTTGATTCACAAAAGGCTGCA
ACAAAGCAAGTCAACAAGGCACACAATAGGTTAATCGAAAAAAAAGTCCA
CAGTGAGAGAAGCGCTGAGTCCTGTGCAACATGGAAAGTGCAGAAAGAGG
AGAAACTGATTGGATTGATGAACAATGAAGCAGCTCAGGAAAGCACAAGG
AGAAGACAGAAATATCAAGAGTCTCAAGAACCTTTGAATGAAGTAAACAG
TTTCCCACAAAAAATCAGCTATACACAATTCCCAAACTCATTTTATGAGC
CTCATCAAGATTTTACCAGTCCAGATATATTCAAGAAGTCAAGATCTCCA
TCTTGGTATAAATACACTTCCACAGTCAGCACGGGGATCACAGACTTAGA
AAGTTCAACTGGACTTTGGCCTACAATTTCCCAGTTTACTCTTAGTGAAG
AGACAAACGCAGATGTTTATTATTATCGCATCATCATACCTGTCCTTTTG
ATGTTAGTATTTCTTGCTTTATTTTTTCTCTGAgcggccgc

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMPPKR
NEKYKLPIPFPEGKVLDDMEGNQWVLGKKIGSGGFGLIYLAFPTNKPEKD
ARHVVKVEYQENGPLFSELKFYQRVAKKDCIKKWIERKQLDYLGIPLFYG
SGLTEFKGRSYRFMVMERLGIDLQKISGQNGTFKKSTVLQLGIRMLDVLE
YIHENEYVHGDIKAANLLLGYKNPDQVYLADYGLSYRYCPNGNHKQYQEN
PRKGHNGTIEFTSLDAHKGVALSRRSDVEILGYCMLRWLCGKLPWEQNLK
DPVAVQTAKTNLLDELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPN
YQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKA
HNRLIEKKVHSERSAESCATWKVQKEEKLIGLMNNEAAQESTRRRQKYQE
SQEPLNEVNSFPQKISYTQFPNSFYEPHQDFTSPDIFKKSRSPSWYKYTS
TVSTGITDLESSTGLWPTISQFTLSEETNADVYYYRIIIPVLLMLVFLAL
FFL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65104
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: isoform 1; parental 33932
• Low Temp: No