cDNA Clone PUP1/YOR157C

• Plasmid: p416 GPD (LMB) 800-PUP1/YOR157C-300
• Parent Plasmid: p416 GPD (LMB)
• Expressed: PUP1/YOR157C
• Tag: GST
• Species: Yeast

Sequence of Insert

ggatccTTGTCCTCTTCTGCAAATACTCGTGTTTGTGTAATAGCCTAGCCTCTTCCTTGCGAAATGCTTCAATCAAAAACTTTCTTCTTAATTCAGCTTTTTGTAATCTCACTTTCTGTGCTGAACCGTTACTGGATTTTATACTCTGGGGTTCAGCAACGGTCTTCGCAATGAGATTATCCACGTCGATAAAGTTTACCTTGGGTGATAATCTATGTGATCCTTTTGGATGTTTTACACCTTTGGCATACCCAATACCAAGAACACCCTTGGGCTTGGGTGATTTTACCTTATTAATAATATCAGTCTGCTTTGTAGTGGGGCTCTTCAGAATGGACCTTGTTGTGGGGAAAACCCCACTTTTAAAACCATACTTGGCCGCACCTTTCCCCATACTTCAATTATCCTTGTTATTATTGTTGTTGACAATCAGCAATGAAACTATTACCTAAATAATCCATATGTAGATTCTCTTCTTTATTCTACTCCTCTTGGGAATCACTTTCCTACTCGGCGACTTCAGGGTTCTACTTGCACTTCCGTTGCTAGTGATTTTTCATTAGGGCCAATATTTTTACCCTCATTACAAACCCTTTATTGTAAAAGCGGGTAAATTAGGGCCATGTTTCGTATTTTATAACCCGGCCGAGTGGCAAAATACCAAAAAGAAAGATGAAAAGGATAAACTAATAATTCAGCAGAAGGGTGATCAGTTGAGGTGGAGAGTTTGTATAGTAAAGCGTTAAATTTTTTCAGGAATACTCCCCCCACATAAACCTGATTGTAGAAAATAGAATTGAGTGAGCATGGCAGGTTTATCGTTCGACAATTATCAAAGAAATAATTTCTTAGCGGAAAACTCGCACACACAACCTAAGGCAACTTCCACGGGTACCACCATTGTAGGCGTAAAATTCAATAACGGAGTGGTGATTGCAGCAGATACAAGATCAACACAAGGTCCAATTGTCGCAGATAAGAATTGCGCAAAATTGCATAGAATTTCCCCCAAAATATGGTGTGCTGGTGCCGGTACCGCCGCCGATACTGAGGCAGTTACGCAGTTGATCGGGTCTAACATTGAATTACATTCCTTATATACCTCGAGAGAACCAAGAGTGGTCTCAGCTTTACAAATGCTAAAGCAGCACCTATTTAAGTACCAAGGTCATATTGGTGCTTATTTGATTGTTGCAGGTGTAGACCCTACGGGATCTCATTTGTTCTCCATTCATGCGCATGGTTCTACTGACGTGGGCTACTACCTTTCTCTAGGATCTGGTTCTCTAGCGGCAATGGCTGTGTTGGAATCACACTGGAAGCAAGACCTTACGAAGGAGGAAGCCATAAAGTTGGCCTCTGATGCAATCCAGGCTGGTATCTGGAATGATTTGGGGTCTGGTTCTAATGTGGATGTGTGTGTCATGGAAATAGGTAAAGATGCAGAATACTTGAGGAATTACTTGACTCCAAATGTTAGAGAAGAAAAGCAGAAAAGCTACAAATTCCCCAGGGGTACAACTGCTGTGCTGAAGGAGAGTATTGTCAATATTTGTGACATACAAGAAGAACAAGTCGATATAACGGCTTGAAAGTAAAACATAGAACTTAAGTATATTTTAGTATAGTAAATCATTGTTCAACAAAATGTGATAAAGGAAGTCAAAAACTAACCGCAAAACTATTTACATAATATATTTTCTTATAGCAGAGCTTATTATTTGTTCTTGATTCTTTTTCTTTTTTCTTTTTTTTATGCTTTGAAGTGTGTTAGTTGATTTTTGTTCGTTAATTTTCGTGTTAATTTATCCATCCATTTTAGCTATTTTATCACACAGAGCATAATATAACAAACACAGCTCAGTACCACACATATAACATAACACTAAAATcccggg

Amino Acid Sequence

MAGLSFDNYQRNNFLAENSHTQPKATSTGTTIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCAGAGTAADTEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHIGAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGSLAAMAVLESHWKQDLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPNVREEKQKSYKFPRGTTAVLKESIVNICDIQEEQVDITA*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU66054
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: PUP1/YOR157C (ID: 854328) (including 800bp of the 5'UTR and 300 bp of the 3'UTR) in p416-GPD (DU66025) vector.
• Low Temp: No