cDNA Clone FAM83G

• Synonyms: PAWS1
• Plasmid: pETDuet mMBP 3C FAM83G 24-307/6His CSNK1A1 isoform2
• Parent Plasmid: pETDuet1
• Expressed: mMBP 3C FAM83G 24-307
• Genbank: NM_001039999.2
• Tag: MBP
• Species: Human

Sequence of Insert

CCATGGGCATGAAGACCGAGGAGGGCAAGCTCGTCATCTGGATCAACGGCGACAAGGGCTACAACGGCCTCGCCGAGGTCGGCAAGAAGTTCGAGAAGGACACCGGCATCAAGGTCACCGTCGAGCACCCCGACAAGCTCGAGGAGAAGTTCCCCCAGGTCGCCGCCACCGGCGACGGCCCCGACATCATCTTCTGGGCCCACGACCGCTTCGGCGGCTACGCCCAGTCCGGCCTCCTCGCCGAGATCACCCCCGCCGCCGCCTTCCAGGACAAGCTCTACCCCTTCACCTGGGACGCCGTCCGCTACAACGGCAAGCTCATCGCCTACCCCATCGCCGTCGAGGCCCTCTCCCTCATCTACAACAAGGACCTCCTCCCCAACCCCCCCAAGACCTGGGAGGAGATCCCCGCCCTCGACAAGGAGCTCAAGGCCAAGGGCAAGTCCGCCCTCATGTTCAACCTCCAGGAGCCCTACTTCACCTGGCCCCTCATCGCCGCCGACGGCGGCTACGCCTTCAAGTACGCCGCCGGCAAGTACGACATCAAGGACGTCGGCGTCGACAACGCCGGCGCCAAGGCCGGCCTCACCTTCCTCGTCGACCTCATCAAGAACAAGCACATGAACGCCGACACCGACTACTCCATCGCCGAGCACGCCTTCAACCACGGCGAGACCGCCATGACCATCAACGGCCCCTGGGCCTGGTCCAACATCGACACCTCCGCCGTCAACTACGGCGTCACCGTCCTCCCCACCTTCAAGGGCCAGCCCTCCAAGCCCTTCGTCGGCGTCCTCTCCGCCGGCATCAACGCCGCCTCCCCCAACAAGGAGCTCGCCAAGGAGTTCCTCGAGAACTACCTCCTCACCGACGAGGGCCTCGAGGCCGTCAACAAGGACAAGCCCCTCGGCGCCGTCGCCCTCAAGTCCTACGAGGAGGAGCTCGTCAAGGACCCCCGCGTCGCCGCCACAATGGAGAACGCCCAGAAGGGCGAGATCATGCCCAACATCCCCCAGATGTCCGCCTTCTGGTACGCCGTCCGCACCGCCGTCATCAACGCCGCCTCCGGCCGCCAGACCGTCGACGCCGCCCTCGCCGCCGCCCAGACCCTGGAAGTTCTGTTCCAGGGGCCCGGATCCGAGTTCTTCTACAGCGAGGAGCAGCGGCTGGCGCTGGAGGCCCTGGTGGCCCGCGGCCGGGACGCCTTCTACGAGGTGCTCAAGCGGGAGAACATCCGAGACTTCCTCTCGGAGCTGGAGCTCAAGCGCATCCTGGAGACCATCGAGGTGTACGACCCGGGCTCTGAGGACCCTCGGGGCACGGGCCCCTCTCAGGGGCCCGAGGACAATGGGGTCGGCGACGGCGAGGAAGCCAGCGGGGCGGATGGGGTCCCCATCGAGGCCGAGCCGCTGCCCTCCCTGGAGTACTGGCCCCAGAAGTCGGACCGCTCCATCCCGCAGCTGGACCTGGGCTGGCCCGACACCATCGCCTACCGCGGCGTGACCCGGGCTAGCGTCTACATGCAGCCCCCCATAGACGGGCAGGCCCACATCAAAGAGGTGGTGCGGAAGATGATCAGCCAGGCACAGAAGGTGATAGCTGTGGTCATGGACATGTTCACCGACGTGGACATCTTCAAGGACCTGCTGGACGCCGGCTTCAAGAGGAAAGTGGCCGTGTACATCATCGTGGATGAGAGTAACGTCAAGTACTTCCTGCACATGTGTGAGCGGGCCTGCATGCACCTGGGGCACCTCAAGAATCTCAGAGTGCGGAGCAGCGGGGGAACTGAGTTCTTCACGCGGTCGGCAACCAAGTTCAAGGGTGCCCTGGCCCAGAAGTTCATGTTTGTGGATGGAGACCGGGCTGTGTGCGGCTCCTACAGCTTCACGTGGTCGGCCGCGCGGACGGACCGGAATGTGATCTCTGTGCTGTCTGGCCAGGTGGTGGAGATGTTTGACCGGCAGTTCCAGGAGCTGTACCTCATGTCATGAAGATCT

Amino Acid Sequence

MGMKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPAAAFQDKLYPFTWDAV
RYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVD
LIKNKHMNADTDYSIAEHAFNHGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDK
PLGAVALKSYEEELVKDPRVAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALAAAQTLEVLFQGPGSEFFYSEEQRLALEALVARGRD
AFYEVLKRENIRDFLSELELKRILETIEVYDPGSEDPRGTGPSQGPEDNGVGDGEEASGADGVPIEAEPLPSLEYWPQKSDRSIPQLDLGWPDTIAYRGV
TRASVYMQPPIDGQAHIKEVVRKMISQAQKVIAVVMDMFTDVDIFKDLLDAGFKRKVAVYIIVDESNVKYFLHMCERACMHLGHLKNLRVRSSGGTEFFT
RSATKFKGALAQKFMFVDGDRAVCGSYSFTWSAARTDRNVISVLSGQVVEMFDRQFQELYLMS*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pETDuet mMBP 3C FAM83G 24-307/6His CSNK1A1 isoform2 209.34 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU67020
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: 6His CSNK1A1 isoform2 cloned into DU63997 (pETDuet mMBP 3C FAM83G 24-307) NdeI/KpnI
• Low Temp: No