cDNA Clone 2x(6His)-ULK1

• Plasmid: pcDNA5-FRT/TO-2x(6His)-FLAG-ULK1
• Parent Plasmid: pcDNA5-FRT/TO
• Expressed: 2x(6His)-FLAG-ULK1
• Genbank: NM_003565.3
• Tag: FLAG
• Tag: 6His
• Species: Human

Sequence of Insert

ggatccATGcatcatcatcatcatcatagcagcggcctggtgccgcgcgg
cagccatatggcgagccatcatcatcatcatcatcatcatcatcatggcg
aaaacctgtattttcagggcATGGACTACAAGGACGATGACGATAAGgga
tctATGGAGCCCGGCCGCGGCGGCACAGAGACCGTGGGCAAGTTCGAGTT
CTCCCGCAAGGACCTGATCGGCCACGGCGCCTTCGCGGTGGTCTTCAAGG
GCCGCCACCGCGAGAAGCACGATTTGGAGGTCGCCGTCAAGTGCATTAAC
AAGAAGAACCTCGCCAAGTCTCAGACGCTGCTGGGGAAGGAAATCAAAAT
CCTGAAGGAACTGAAACATGAAAACATCGTGGCCCTGTACGACTTCCAGG
AAATGGCTAATTCTGTCTACCTGGTTATGGAGTACTGCAACGGTGGGGAC
CTGGCCGACTACCTGCACGCCATGCGCACGCTGAGCGAGGACACCATCAG
GCTCTTCCTGCAGCAGATCGCGGGCGCCATGCGGCTTCTGCACAGCAAAG
GCATCATCCACCGCGACCTGAAACCGCAGAACATCCTGCTGTCCAACCCC
GCCGGCCGCCGCGCCAACCCCAACAGCATCCGCGTCAAGATCGCTGACTT
CGGCTTCGCGCGGTACCTCCAGAGCAACATGATGGCGGCCACACTCTGCG
GCTCCCCCATGTACATGGCCCCCGAGGTCATCATGTCCCAGCACTACGAC
GGGAAGGCGGACCTGTGGAGCATCGGCACCATCGTCTACCAGTGCCTGAC
GGGGAAGGCGCCCTTCCAGGCCAGCAGCCCCCAGGACCTGCGCCTGTTCT
ACGAGAAGAACAAGACGTTGGTCCCCACCATCCCCCGGGAGACCTCGGCC
CCGCTGCGGCAGCTGCTCCTGGCCCTACTGCAACGCAACCACAAGGACCG
CATGGACTTCGATGAGTTTTTTCATCACCCTTTCCTCGATGCCAGCCCCT
CGGTCAGGAAATCCCCACCCGTGCCTGTGCCCTCGTACCCAAGCTCGGGG
TCCGGCAGCAGCTCCAGCAGCAGCTCCACCTCCCACCTGGCCTCCCCGCC
GTCCCTGGGCGAGATGCAGCAGCTGCAGAAGACCCTGGCCTCCCCGGCTG
ACACCGCTGGCTTCCTGCACAGCTCCCGGGACTCTGGTGGCAGCAAGGAC
TCTTCCTGTGACACAGATGACTTCGTCATGGTCCCCGCGCAGTTTCCAGG
TGACCTGGTGGCTGAGGCGCCCAGTGCCAAACCCCCGCCAGACAGCCTGA
TGTGCAGTGGGAGCTCACTGGTGGCCTCTGCGGGCTTGGAGAGCCACGGC
CGGACCCCATCTCCATCCCCACCCTGCAGCAGCTCCCCCAGTCCCTCAGG
CCGGGCTGGCCCGTTCTCCAGCAGCAGGTGCGGCGCCTCTGTCCCCATCC
CAGTCCCCACGCAGGTGCAGAACTACCAGCGCATTGAGCGAAACCTGCAG
TCACCCACCCAGTTCCAAACACCTCGGTCCTCTGCCATCCGCAGGTCAGG
CAGCACCAGCCCCCTGGGCTTTGCAAGGGCCAGCCCCTCGCCCCCTGCCC
ACGCTGAGCATGGAGGCGTCCTGGCCAGGAAGATGTCTCTGGGTGGAGGC
CGGCCCTACACGCCATCTCCTCAAGTTGGAACCATCCCTGAGCGGCCAGG
CTGGAGCGGGACGCCCTCCCCACAGGGAGCTGAGATGCGGGGTGGCAGGT
CCCCTCGTCCAGGCTCCTCTGCACCCGAGCACTCTCCCCGCACTTCCGGG
CTGGGCTGCCGCCTGCACAGCGCCCCCAACCTGTCTGACTTGCACGTCGT
CCGCCCCAAGCTGCCCAAACCCCCCACGGACCCCCTGGGAGCTGTGTTCA
GCCCACCACAGGCCAGCCCTCCCCAGCCGTCCCACGGCCTGCAGTCCTGC
CGGAACCTGCGGGGCTCACCCAAGCTGCCCGACTTCCTGCAGCGAAACCC
CCTGCCCCCCATCCTGGGCTCCCCCACCAAGGCTGTGCCCTCCTTTGACT
TCCCGAAGACCCCCAGCTCCCAGAACCTGCTGGCCCTCCTAGCCCGGCAG
GGCGTGGTGATGACGCCCCCTCGAAACCGGACGCTGCCCGACCTCTCGGA
GGTGGGACCCTTCCATGGTCAGCCGTTGGGCCCTGGCCTGCGGCCAGGCG
AGGACCCCAAGGGCCCCTTTGGCCGGTCTTTCAGCACCAGCCGCCTCACT
GACCTGCTCCTTAAGGCGGCGTTTGGGACACAAGCCCCGGACCTGGGCAG
CACGGAGAGCCTGCAGGAGAAGCCCATGGAGATCGCACCCTCAGCTGGCT
TTGGAGGGAGCCTGCACCCAGGAGCCCGTGCTGGGGGCACCAGCAGCCCT
TCCCCGGTGGTCTTCACCGTGGGCTCTCCCCCGAGCGGGAGCACGCCCCC
CCAGGGCCCCCGCACCAGGATGTTCTCAGCGGGCCCCACTGGCTCTGCCA
GCTCTTCTGCCCGCCACCTGGTGCCTGGGCCCTGCAGCGAGGCCCCAGCC
CCTGAGCTCCCTGCTCCAGGACACGGCTGCAGCTTTGCCGACCCCATTGC
TGCGAACCTGGAGGGGGCTGTGACCTTCGAGGCCCCCGACCTCCCTGAGG
AGACCCTCATGGAGCAAGAGCACACGGAGATCCTGCGTGGCCTGCGCTTC
ACGCTGCTGTTCGTGCAGCACGTCCTGGAGATCGCAGCCCTGAAGGGCAG
CGCCAGTGAGGCGGCGGGGGGCCCTGAGTACCAGCTGCAGGAGAGTGTGG
TGGCCGACCAGATCAGCCTGCTGAGCCGAGAATGGGGCTTCGCGGAACAG
CTGGTGCTGTACCTGAAGGTGGCCGAGCTACTGTCCTCCGGCCTGCAAAG
TGCCATCGACCAGATCCGGGCCGGCAAGCTCTGCCTGTCGTCCACTGTGA
AGCAGGTGGTGCGCAGGCTGAATGAGCTGTACAAGGCCAGCGTGGTGTCC
TGCCAGGGCCTGAGCCTGCGGCTGCAGCGCTTCTTCCTGGACAAGCAGCG
GCTCCTGGACCGCATTCACAGCATCACTGCCGAGAGGCTCATCTTCAGCC
ACGCTGTGCAGATGGTGCAGTCGGCTGCCCTGGACGAGATGTTCCAGCAC
CGTGAGGGCTGCGTCCCACGCTACCACAAGGCCCTGCTGCTCCTGGAGGG
GCTGCAGCACATGCTCTCGGACCAGGCCGACATCGAGAACGTCACCAAGT
GCAAGCTGTGCATTGAGCGGAGACTCTCGGCGCTGCTGACTGGCATCTGT
GCCTGAGCGGCCGC

Amino Acid Sequence

MHHHHHHSSGLVPRGSHMASHHHHHHHHHHGENLYFQGMDYKDDDDKGSM
EPGRGGTETVGKFEFSRKDLIGHGAFAVVFKGRHREKHDLEVAVKCINKK
NLAKSQTLLGKEIKILKELKHENIVALYDFQEMANSVYLVMEYCNGGDLA
DYLHAMRTLSEDTIRLFLQQIAGAMRLLHSKGIIHRDLKPQNILLSNPAG
RRANPNSIRVKIADFGFARYLQSNMMAATLCGSPMYMAPEVIMSQHYDGK
ADLWSIGTIVYQCLTGKAPFQASSPQDLRLFYEKNKTLVPTIPRETSAPL
RQLLLALLQRNHKDRMDFDEFFHHPFLDASPSVRKSPPVPVPSYPSSGSG
SSSSSSSTSHLASPPSLGEMQQLQKTLASPADTAGFLHSSRDSGGSKDSS
CDTDDFVMVPAQFPGDLVAEAPSAKPPPDSLMCSGSSLVASAGLESHGRT
PSPSPPCSSSPSPSGRAGPFSSSRCGASVPIPVPTQVQNYQRIERNLQSP
TQFQTPRSSAIRRSGSTSPLGFARASPSPPAHAEHGGVLARKMSLGGGRP
YTPSPQVGTIPERPGWSGTPSPQGAEMRGGRSPRPGSSAPEHSPRTSGLG
CRLHSAPNLSDLHVVRPKLPKPPTDPLGAVFSPPQASPPQPSHGLQSCRN
LRGSPKLPDFLQRNPLPPILGSPTKAVPSFDFPKTPSSQNLLALLARQGV
VMTPPRNRTLPDLSEVGPFHGQPLGPGLRPGEDPKGPFGRSFSTSRLTDL
LLKAAFGTQAPDLGSTESLQEKPMEIAPSAGFGGSLHPGARAGGTSSPSP
VVFTVGSPPSGSTPPQGPRTRMFSAGPTGSASSSARHLVPGPCSEAPAPE
LPAPGHGCSFADPIAANLEGAVTFEAPDLPEETLMEQEHTEILRGLRFTL
LFVQHVLEIAALKGSASEAAGGPEYQLQESVVADQISLLSREWGFAEQLV
LYLKVAELLSSGLQSAIDQIRAGKLCLSSTVKQVVRRLNELYKASVVSCQ
GLSLRLQRFFLDKQRLLDRIHSITAERLIFSHAVQMVQSAALDEMFQHRE
GCVPRYHKALLLLEGLQHMLSDQADIENVTKCKLCIERRLSALLTGICA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65171
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 41459; Grow at 30 degrees silent change c1065t and coding change c2141t (P714L) (exisiting SNP)
• Low Temp: No