cDNA Clone GPNMB

• Plasmid: pSC-B-GPNMB tv1
• Parent Plasmid: pSC-B
• Genbank: NM_001005340.1 → NP_001005340.1
• Species: Human

Sequence of Insert

gtcgacATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGC
AAGATTGCCACTTGATGCCGCCAAACGATTTCATGATGTGCTGGGCAATG
AAAGACCTTCTGCTTACATGAGGGAGCACAATCAATTAAATGGCTGGTCT
TCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGAAGCGGGG
AGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCC
TGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTG
AACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGT
CTATGAGAAGAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATG
TTTACAACTGGACAGCATGGTCAGAGGACAGTGACGGGGAAAATGGCACC
GGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTCCTCACCA
CCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTC
AGTATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACA
GCCAATGTGACACTTGGGCCTCAACTCATGGAAGTGACTGTCTACAGAAG
ACATGGACGGGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGG
TAACAGATCAGATTCCTGTGTTTGTGACTATGTTCCAGAAGAACGATCGA
AATTCATCCGACGAAACCTTCCTCAAAGATCTCCCCATTATGTTTGATGT
CCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAACTACA
AGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACT
GTGAATCACACGTATGTGCTCAATGGAACCTTCAGCCTTAACCTCACTGT
GAAAGCTGCAGCACCAGGACCTTGTCCGCCACCGCCACCACCACCCAGAC
CTTCAAAACCCACCCCTTCTTTAGCAACTACTCTAAAATCTTATGATTCA
AACACCCCAGGACCTGCTGGTGACAACCCCCTGGAGCTGAGTAGGATTCC
TGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACCATCA
CAATTGTAGAGGGAATCTTAGAGGTTAACATCATCCAGATGACAGACGTC
CTGATGCCGGTGCCATGGCCTGAAAGCTCCCTAATAGACTTTGTCGTGAC
CTGCCAAGGGAGCATTCCCACGGAGGTCTGTACCATCATTTCTGACCCCA
CCTGCGAGATCACCCAGAACACAGTCTGCAGCCCTGTGGATGTGGATGAG
ATGTGTCTGCTGACTGTGAGACGAACCTTCAATGGGTCTGGGACGTACTG
TGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCACGAGCACCC
TGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAAC
AGTGCCCTGATCTCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTC
CCTCTTGGTGTACAAAAAACACAAGGAATACAACCCAATAGAAAATAGTC
CTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGCA
AAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAA
CCAAGAATTTAAAGGAGTTTCTgcggccgc

Amino Acid Sequence

MECLYYFLGFLLLAARLPLDAAKRFHDVLGNERPSAYMREHNQLNGWSSD
ENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNL
IFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQ
SHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTAN
VTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNS
SDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVN
HTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLATTLKSYDSNT
PGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLM
PVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMC
LLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVPDRDPASPLRMANSA
LISVGCLAIFVTVISLLVYKKHKEYNPIENSPGNVVRSKGLSVFLNRAKA
VFFPGNQEKDPLLKNQEFKGVS

• Vector Type: Mammalian
• Antibiotic: Amp; Kan
• DU Number: DU65221
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: tv1 created by insertion mutagenesis of Ala340-Pro351 into tv2 precursor; no stop codon
• Low Temp: No