cDNA Clone GPNMB

• Plasmid: pBabe.puro-GPNMB-C-GFP
• Parent Plasmid: pBabe.puro-DU C-Term GFP
• Expressed: GPNMB-GFP
• Genbank: NM_001005340.1 → NP_001005340.1
• Tag: GFP
• Species: Human

Sequence of Insert

gtcgacATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCCTGGCTGC
AAGATTGCCACTTGATGCCGCCAAACGATTTCATGATGTGCTGGGCAATG
AAAGACCTTCTGCTTACATGAGGGAGCACAATCAATTAAATGGCTGGTCT
TCTGATGAAAATGACTGGAATGAAAAACTCTACCCAGTGTGGAAGCGGGG
AGACATGAGGTGGAAAAACTCCTGGAAGGGAGGCCGTGTGCAGGCGGTCC
TGACCAGTGACTCACCAGCCCTCGTGGGCTCAAATATAACATTTGCGGTG
AACCTGATATTCCCTAGATGCCAAAAGGAAGATGCCAATGGCAACATAGT
CTATGAGAAGAACTGCAGAAATGAGGCTGGTTTATCTGCTGATCCGTATG
TTTACAACTGGACAGCATGGTCAGAGGACAGTGACGGGGAAAATGGCACC
GGCCAAAGCCATCATAACGTCTTCCCTGATGGGAAACCTTTTCCTCACCA
CCCCGGATGGAGAAGATGGAATTTCATCTACGTCTTCCACACACTTGGTC
AGTATTTCCAGAAATTGGGACGATGTTCAGTGAGAGTTTCTGTGAACACA
GCCAATGTGACACTTGGGCCTCAACTCATGGAAGTGACTGTCTACAGAAG
ACATGGACGGGCATATGTTCCCATCGCACAAGTGAAAGATGTGTACGTGG
TAACAGATCAGATTCCTGTGTTTGTGACTATGTTCCAGAAGAACGATCGA
AATTCATCCGACGAAACCTTCCTCAAAGATCTCCCCATTATGTTTGATGT
CCTGATTCATGATCCTAGCCACTTCCTCAATTATTCTACCATTAACTACA
AGTGGAGCTTCGGGGATAATACTGGCCTGTTTGTTTCCACCAATCATACT
GTGAATCACACGTATGTGCTCAATGGAACCTTCAGCCTTAACCTCACTGT
GAAAGCTGCAGCACCAGGACCTTGTCCGCCACCGCCACCACCACCCAGAC
CTTCAAAACCCACCCCTTCTTTAGCAACTACTCTAAAATCTTATGATTCA
AACACCCCAGGACCTGCTGGTGACAACCCCCTGGAGCTGAGTAGGATTCC
TGATGAAAACTGCCAGATTAACAGATATGGCCACTTTCAAGCCACCATCA
CAATTGTAGAGGGAATCTTAGAGGTTAACATCATCCAGATGACAGACGTC
CTGATGCCGGTGCCATGGCCTGAAAGCTCCCTAATAGACTTTGTCGTGAC
CTGCCAAGGGAGCATTCCCACGGAGGTCTGTACCATCATTTCTGACCCCA
CCTGCGAGATCACCCAGAACACAGTCTGCAGCCCTGTGGATGTGGATGAG
ATGTGTCTGCTGACTGTGAGACGAACCTTCAATGGGTCTGGGACGTACTG
TGTGAACCTCACCCTGGGGGATGACACAAGCCTGGCTCTCACGAGCACCC
TGATTTCTGTTCCTGACAGAGACCCAGCCTCGCCTTTAAGGATGGCAAAC
AGTGCCCTGATCTCCGTTGGCTGCTTGGCCATATTTGTCACTGTGATCTC
CCTCTTGGTGTACAAAAAACACAAGGAATACAACCCAATAGAAAATAGTC
CTGGGAATGTGGTCAGAAGCAAAGGCCTGAGTGTCTTTCTCAACCGTGCA
AAAGCCGTGTTCTTCCCGGGAAACCAGGAAAAGGATCCGCTACTCAAAAA
CCAAGAATTTAAAGGAGTTTCTgcggccgc

Amino Acid Sequence

MECLYYFLGFLLLAARLPLDAAKRFHDVLGNERPSAYMREHNQLNGWSSD
ENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNL
IFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQ
SHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTAN
VTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNS
SDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVN
HTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLATTLKSYDSNT
PGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLM
PVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMC
LLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVPDRDPASPLRMANSA
LISVGCLAIFVTVISLLVYKKHKEYNPIENSPGNVVRSKGLSVFLNRAKA
VFFPGNQEKDPLLKNQEFKGVSAAAMVSKGEELFTGVVPILVELDGDVNG
HKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRY
PDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
LKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDG
SVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFV
TAAGITLGMDELYK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65255
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: tv1 ; Parental 40432
• Low Temp: No