cDNA Clone RNF12

• Synonyms: MRX61; RLIM; TOKAS; NY-REN-43
• Plasmid: pCAGGSD puro RNF12 Y421C
• Parent Plasmid: pCAGGSD puro
• Expressed: RNF12 Y421C
• Genbank: NM_016120.3
• Species: Human

Sequence of Insert

GGATCCATGGAAAACTCAGATTCCAATGACAAAGGAAGTGGTGATCAGTCTGCAGCACAGCGCAGAAGTCAGATGGACCGATTGGATCGAGAAGAAGCTTTCTATCAATTTGTAAATAACCTGAGTGAAGAAGATTATAGGCTTATGAGAGATAACAATTTGCTAGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTGAGACGACTACAGCAAATTAAAGAAGGCCCACCACCGCAAAACTCAGATGAAAATAGAGGAGGAGACTCTTCAGATGATGTGTCTAATGGTGACTCTATAATAGACTGGCTTAACTCTGTCAGACAAACTGGAAATACAACAAGAAGTGGGCAAAGAGGAAACCAATCTTGGAGAGCAGTGAGTCGGACTAATCCAAACAGTGGTGATTTCAGATTCAGTTTAGAGATAAATGTTAACCGTAATAATGGGAGCCAAAATTCAGAGAATGAAAATGAGCCATCTGCAAGACGTTCTAGTGGAGAAAATGTGGAAAACAACAGCCAAAGGCAAGTGGAAAACCCACGATCTGAATCAACATCTGCAAGGCCATCTAGATCAGAACGAAATTCAACTGAAGCGTTAACAGAGGTCCCACCTACCAGAGGTCAGAGGAGGGCAAGAAGCAGGAGCCCAGACCATCGGAGAACCAGAGCAAGAGCTGAAAGAAGTAGGTCACCTCTGCATCCAATGAGTGAAATTCCACGAAGATCTCATCATAGTATCTCATCTCAGACTTTTGAACATCCTTTGGTAAATGAGACGGAGGGAAGTTCTAGAACCCGGCACCATGTGACATTGAGGCAGCAAATATCTGGGCCTGAGTTGCTAAGTAGAGGTCTTTTTGCAGCTTCTGGAACAAGAAATGCTTCTCAAGGAGCAGGTTCTTCAGACACAGCTGCCAGTGGTGAATCTACAGGATCAGGACAGAGACCTCCAACCATAGTCCTTGATCTTCAAGTAAGAAGAGTTCGTCCTGGAGAATATCGGCAGAGAGATAGCATAGCCAGCAGAACTCGGTCTAGGTCTCAGACACCAAACAACACTGTCACCTATGAAAGTGAACGAGGAGGTTTTAGGCGTACATTTTCACGTTCTGAGCGGGCAGGTGTGAGAACCTATGTCAGTACCATCAGAATTCCCATTCGTAGAATCTTAAATACTGGTTTAAGTGAGACTACATCTGTTGCAATTCAGACCATGTTAAGGCAGATAATGACAGGTTTTGGTGAGTTAAGCTATTTTATGTGCAGTGATAGCGACTCAGAGCCTACTGGCTCAGTCTCAAATCGAAATATGGAAAGGGCAGAGTCACGGAGTGGAAGAGGAGGTTCTGGTGGTGGTAGTAGTTCTGGTTCCAGTTCGAGTTCCAGTTCCAGTTCGAGTTCCAGTTCCAGTTCAAGTTCCAGTTCCAGTCCTAGTTCCAGTTCCGGTGGTGAAAGTTCAGAAACTAGCTCAGATTTATTTGAAGGCAGTAATGAAGGAAGCTCATCATCAGGCTCATCAGGTGCCAGGCGAGAGGGTCGACATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTTTGCCCTTCCTTAGCCTGGCTCAGTTTTTCCTCTTAAATGAGGATGATGATGACCAACCTAGAGGACTCACCAAAGAACAGATTGACAACTTGGCAATGAGAAGTTTTGGTGAAAATGATGCATTAAAAACCTGTAGTGTTTGCATTACAGAATATACAGAAGGCAACAAACTTCGTAAACTACCTTGTTCCCATGAGTACCATGTCCACTGCATCGATCGCTGGTTATCTGAGAATTCTACCTGTCCTATTTGTCGCAGAGCAGTCTTAGCTTCTGGTAACAGAGAAAGTGTTGTGTAAGCGGCCGC

Amino Acid Sequence

MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIID
WLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALT
EVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGS
SDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSV
AIQTMLRQIMTGFGELSYFMCSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGS
SSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRW
LSENSTCPICRRAVLASGNRESVV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU67254
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Recloning of insert from DU61099 (which shows poor expression levels) to eliminate possibility of error in backbone.; Update June 2019: This clone also has low expression levels.
• Low Temp: No