cDNA Clone LRRK1

• Plasmid: pcDNA5D FRT/TO GFP LRRK1 Y1410F
• Parent Plasmid: pcDNA5D FRT/TO GFP
• Expressed: GFP-LRRK1 Y1410F
• Genbank: NM_024652
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCATGGCTGGCATGTCGCAAAGACCCCCCAGCATGTACTGGTGTGTGGGGCCGGAGGAGTCAGCTGTGTGTCCAGAACGTGCCATGGAGACGCTTAACGGTGCCGGGGACACGGGCGGCAAGCCGTCCACGCGGGGCGGTGACCCTGCAGCGCGGTCCCGCAGGACGGAAGGCATCCGCGCCGCGTACAGGCGGGGAGACCGCGGCGGCGCCCGGGACCTGCTGGAGGAGGCCTGCGACCAGTGCGCGTCCCAGCTGGAAAAGGGCCAGCTTCTGAGCATCCCGGCAGCCTATGGGGATCTGGAGATGGTCCGCTACCTACTCAGCAAGAGACTGGTGGAGCTGCCCACCGAGCCCACGGATGACAACCCAGCCGTGGTGGCAGCGTATTTTGGACACACGGCAGTTGTGCAGGAATTGCTTGAGTCCTTACCAGGTCCCTGCAGTCCCCAGCGGCTTCTGAACTGGATGCTGGCCTTGGCTTGCCAGCGAGGGCACCTGGGGGTTGTGAAGCTCCTGGTCCTGACGCACGGGGCTGACCCGGAGAGCTACGCTGTCAGGAAGAATGAGTTCCCTGTCATCGTGCGCTTGCCCCTGTATGCGGCCATCAAGTCAGGGAATGAAGACATTGCAATATTCCTGCTTCGGCATGGGGCCTATTTCTGTTCCTACATCTTGCTGGATAGTCCTGACCCCAGCAAACATCTGCTGAGAAAGTACTTCATTGAAGCCAGTCCCTTGCCCAGCAGTTATCCGGGAAAAACAGCTCTCCGTGTGAAATGGTCCCATCTCAGACTGCCCTGGGTAGACCTAGACTGGCTCATAGACATCTCCTGCCAGATCACGGAGCTCGACCTTTCTGCCAACTGCCTGGCGACCCTCCCCTCGGTTATCCCCTGGGGCCTCATCAATCTCCGGAAGCTGAACCTCTCCGACAACCACCTGGGGGAGCTGCCTGGCGTGCAGTCATCGGACGAAATCATCTGTTCCAGGCTACTTGAAATTGACATTTCCAGCAACAAGTTGTCCCACCTCCCTCCTGGATTCTTGCACCTCTCAAAACTTCAAAAACTGACAGCTTCAAAAAATTGTTTAGAAAAATTGTTCGAAGAAGAAAATGCCACTAACTGGATAGGTTTACGGAAGCTACAGGAACTTGATATATCTGACAATAAATTGACAGAACTCCCTGCCCTGTTCCTTCACTCTTTCAAGTCCCTCAATTCTCTGAATGTCTCCAGAAACAACCTGAAGGTGTTTCCAGATCCCTGGGCCTGCCCTTTGAAATGTTGTAAAGCTTCCAGAAATGCCCTGGAATGTCTGCCAGACAAAATGGCTGTCTTTTGGAAAAATCACCTGAAGGATGTGGATTTCTCAGAAAACGCACTCAAAGAAGTTCCCCTGGGACTTTTCCAGCTTGATGCCCTCATGTTCTTGAGGTTACAGGGGAACCAGCTGGCGGCACTTCCACCTCAAGAGAAGTGGACCTGCAGGCAGCTCAAAACCCTGGATCTCTCCAGAAACCAACTTGGCAAAAATGAAGATGGACTGAAAACGAAGCGTATTGCCTTTTTCACCACCAGAGGTCGCCAGCGCTCCGGGACTGAGGCAGCAAGTGTGCTGGAATTTCCGGCCTTCCTAAGTGAGTCTTTGGAAGTCCTTTGCCTGAACGACAACCACCTCGACACAGTCCCTCCCTCGGTTTGCCTACTGAAGAGCTTATCAGAGCTCTACTTGGGAAACAACCCTGGCCTCCGGGAGCTCCCTCCTGAGCTGGGGCAGCTGGGCAACCTCTGGCAGCTGGACACTGAAGACCTGACCATCAGCAATGTGCCTGCAGAAATCCAAAAAGAAGGCCCCAAAGCAATGCTGTCTTACCTGCGTGCTCAGCTGCGGAAAGCGGAAAAGTGCAAGCTGATGAAGATGATCATCGTGGGTCCCCCGCGCCAGGGCAAGTCCACCCTCCTGGAGATCTTACAGACGGGGAGGGCCCCCCAGGTGGTGCATGGAGAGGCCACCATCAGGACCACCAAGTGGGAGCTCCAGAGGCCGGCTGGCTCAAGAGCCAAGGTTGAGTCCGTGGAGTTCAACGTCTGGGACATCGGGGGACCGGCCAGCATGGCCACTGTCAACCAGTGCTTCTTCACGGACAAGGCCCTGTACGTGGTGGTCTGGAACCTGGCGCTGGGGGAGGAGGCCGTGGCCAACCTCCAGTTCTGGCTGCTCAACATCGAGGCCAAGGCCCCAAACGCCGTGGTGCTGGTGGTCGGGACGCACCTGGATTTAATTGAAGCCAAGTTCCGTGTGGAAAGGATTGCAACGCTGCGTGCCTATGTGCTGGCACTCTGCCGCTCCCCCTCCGGCTCCAGGGCCACAGGCTTCCCAGACATCACCTTCAAACACTTACATGAGATTTCCTGCAAGAGCCTGGAAGGTCAGGAAGGGCTGCGACAGCTGATTTTCCACGTCACGTGCAGCATGAAGGACGTGGGCAGCACCATCGGCTGCCAGCGACTGGCAGGGCGGCTGATCCCCAGGAGCTACCTGAGCCTGCAGGAGGCCGTGCTGGCAGAGCAGCAGCGCCGCAGCCGGGACGACGACGTGCAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCCGACAACGACATCAAGGACTACGAGGACCTGCAGTCAGCCATCAGCTTCCTCATAGAAACCGGCACCCTGCTCCATTTCCCGGACACCAGCCACGGCCTGAGGAACCTCTACTTCCTCGACCCTATTTGGCTCTCCGAATGTCTGCAGAGGATCTTTAATATTAAGGGCTCTCGGTCAGTGGCCAAGAATGGGGTGATCAGAGCAGAAGACCTCAGGATGCTGCTGGTGGGGACTGGCTTCACGCAGCAGACGGAAGAGCAGTACTTCCAGTTCCTGGCCAAGTTTGAGATCGCCCTGCCCGTCGCCAATGACAGCTACCTCCTGCCCCATCTCCTTCCATCTAAACCTGGCCTGGACACCCACGGTATGCGGCACCCCACAGCCAACACCATTCAGAGGGTATTTAAGATGAGCTTCGTTCCCGTTGGCTTCTGGCAAAGGTTTATAGCACGGATGCTGATCAGCCTGGCGGAGATGGACCTGCAGCTTTTTGAAAACAAGAAGAATACTAAAAGCAGGAACAGGAAAGTCACCATTTACAGTTTTACAGGAAACCAGAGAAATCGCTGTAGCACATTCAGAGTGAAAAGAAATCAGACCATCTATTGGCAGGAAGGGCTCCTGGTCACTTTTGATGGGGGCTACCTCAGTGTGGAATCTTCCGACGTGAACTGGAAAAAGAAGAAAAGCGGAGGAATGAAAATTGTTTGCCAATCAGAAGTGAGGGACTTCTCAGCCATGGCTTTCATCACGGACCACGTCAATTCCTTGATTGATCAGTGGTTTCCCGCCCTGACAGCCACAGAGAGCGACGGGACGCCACTCATGGAGCAGTACGTGCCCTGCCCGGTCTGCGAGACAGCCTGGGCCCAGCACACGGACCCCAGTGAGAAATCAGAGGATGTGCAGTACTTCGACATGGAAGACTGTGTCCTGACGGCCATCGAGCGGGACTTCATCTCCTGCCCCAGACACCCGGACCTCCCCGTGCCGCTGCAGGAGCTGGTCCCTGAACTGTTCATGACCGACTTCCCGGCCAGGCTCTTCCTGGAGAACAGCAAGCTGGAGCACAGCGAGGACGAGGGCAGCGTCCTGGGCCAGGGCGGCAGTGGCACCGTCATCTACCGGGCCCGGTACCAGGGCCAGCCTGTGGCCGTCAAGCGCTTCCACATCAAAAAATTCAAGAACTTTGCTAACGTACCGGCAGACACCATGCTGAGGCACCTGCGGGCCACCGATGCCATGAAGAACTTCTCCGAGTTCCGGCAGGAGGCCAGCATGCTGCACGCGCTGCAGCACCCCTGCATCGTGGCGCTCATCGGCATCAGCATCCACCCGCTCTGCTTCGCCCTGGAGCTCGCGCCGCTCAGCAGCCTCAACACCGTGCTGTCCGAGAACGCCAGAGATTCTTCCTTTATACCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATCGCCTCGGGCCTGGCCTACCTGCACAAGAAAAACATCATCTTCTGTGACCTGAAGTCGGACAACATTCTGGTGTGGTCCCTTGACGTCAAGGAGCACATCAACATCAAGCTATCTGACTTCGGGATTTCGAGGCAGTCATTCCATGAGGGCGCCCTAGGCGTGGAGGGCACTCCTGGCTACCAGGCCCCAGAGATCAGGCCTCGCATTGTATATGATGAGAAGGTAGATATGTTCTCCTATGGAATGGTGCTCTACGAGTTGCTGTCAGGACAGCGCCCTGCACTGGGCCACCACCAGCTCCAGATTGCCAAGAAGCTGTCCAAGGGCATCCGCCCGGTTCTGGGGCAGCCGGAGGAAGTGCAGTTCCGGCGACTGCAGGCGCTCATGATGGAGTGCTGGGACACTAAGCCAGAGAAGCGACCGCTGGCCCTGTCGGTGGTGAGCCAGATGAAGGACCCGACTTTTGCCACCTTCATGTATGAACTGTGCTGTGGGAAGCAGACAGCCTTCTTCTCATCCCAGGGCCAGGAGTACACCGTGGTGTTTTGGGATGGAAAAGAGGAGTCCAGGAACTACACGGTGGTGAACACAGAGAAGGGCCTCATGGAGGTGCAGAGGATGTGCTGCCCTGGGATGAAGGTGAGCTGCCAGCTCCAGGTCCAGAGATCCCTGTGGACAGCCACCGAGGACCAGAAAATCTACATCTACACCCTCAAGGGCATGTGCCCCTTAAACACACCCCAACAGGCCTTGGATACTCCAGCTGTCGTCACCTGCTTCTTGGCCGTGCCTGTTATTAAAAAGAATTCCTACCTGGTCTTAGCGGGCCTCGCCGATGGGCTTGTGGCTGTGTTTCCCGTGGTGCGGGGCACCCCAAAGGACAGCTGCTCCTACCTGTGCTCACACACAGCCAACAGGTCCAAGTTCAGCATCGCGGATGAAGACGCACGGCAGAACCCCTACCCAGTGAAGGCCATGGAGGTGGTCAACAGCGGCTCTGAGGTCTGGTACAGCAATGGGCCGGGCCTCCTTGTCATCGACTGTGCCTCCCTGGAGATCTGCAGGCGGCTGGAGCCCTACATGGCCCCCTCCATGGTTACGTCAGTCGTGTGCAGCTCTGAGGGCAGAGGGGAGGAGGTCGTCTGGTGCCTGGATGACAAGGCCAACTCCTTGGTGATGTACCACTCCACCACCTACCAGCTGTGTGCCCGGTACTTCTGCGGGGTCCCCAGCCCCCTCAGGGACATGTTTCCCGTGCGGCCCTTGGACACGGAACCCCCGGCAGCCAGCCACACGGCCAACCCAAAGGTGCCTGAGGGGGACTCCATCGCGGACGTGAGCATCATGTACAGTGAGGAGCTGGGCACGCAGATCCTGATCCACCAGGAATCACTCACTGACTACTGCTCCATGTCCTCCTACTCCTCATCCCCACCCCGCCAGGCTGCCAGGTCCCCCTCAAGCCTCCCCAGCTCCCCAGCAAGTTCTTCCAGTGTGCCTTTCTCCACCGACTGCGAGGACTCAGACATGCTACATACGCCCGGTGCTGCCTCCGACAGGTCTGAGCATGACCTGACCCCCATGGACGGGGAGACCTTCAGCCAGCACCTGCAGGCCGTGAAGATCCTCGCCGTCAGAGACCTCATTTGGGTCCCCAGGCGCGGTGGAGATGTTATCGTCATTGGCCTGGAGAAGGATTCTGGCGCCCAGCGGGGCCGAGTCATTGCCGTCTTAAAAGCCCGAGAGCTGACTCCGCATGGGGTGCTGGTGGATGCTGCCGTGGTGGCAAAGGACACTGTTGTGTGCACCTTTGAAAATGAAAACACAGAGTGGTGCCTGGCCGTCTGGAGGGGCTGGGGCGCCAGGGAGTTCGACATTTTCTACCAGTCCTACGAGGAGCTGGGCCGGCTGGAGGCTTGCACTCGCAAGAGAAGGTAAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMAGMSQRPPSMYWCVGPEESAVCPERAMETLNGAGDTGGKPSTRGGDPAARSRRTEGIRAAYRRGDRGGARDLLEEACDQCASQLEKGQLLSIPAAYGDLEMVRYLLSKRLVELPTEPTDDNPAVVAAYFGHTAVVQELLESLPGPCSPQRLLNWMLALACQRGHLGVVKLLVLTHGADPESYAVRKNEFPVIVRLPLYAAIKSGNEDIAIFLLRHGAYFCSYILLDSPDPSKHLLRKYFIEASPLPSSYPGKTALRVKWSHLRLPWVDLDWLIDISCQITELDLSANCLATLPSVIPWGLINLRKLNLSDNHLGELPGVQSSDEIICSRLLEIDISSNKLSHLPPGFLHLSKLQKLTASKNCLEKLFEEENATNWIGLRKLQELDISDNKLTELPALFLHSFKSLNSLNVSRNNLKVFPDPWACPLKCCKASRNALECLPDKMAVFWKNHLKDVDFSENALKEVPLGLFQLDALMFLRLQGNQLAALPPQEKWTCRQLKTLDLSRNQLGKNEDGLKTKRIAFFTTRGRQRSGTEAASVLEFPAFLSESLEVLCLNDNHLDTVPPSVCLLKSLSELYLGNNPGLRELPPELGQLGNLWQLDTEDLTISNVPAEIQKEGPKAMLSYLRAQLRKAEKCKLMKMIIVGPPRQGKSTLLEILQTGRAPQVVHGEATIRTTKWELQRPAGSRAKVESVEFNVWDIGGPASMATVNQCFFTDKALYVVVWNLALGEEAVANLQFWLLNIEAKAPNAVVLVVGTHLDLIEAKFRVERIATLRAYVLALCRSPSGSRATGFPDITFKHLHEISCKSLEGQEGLRQLIFHVTCSMKDVGSTIGCQRLAGRLIPRSYLSLQEAVLAEQQRRSRDDDVQYLTDRQLEQLVEQTPDNDIKDYEDLQSAISFLIETGTLLHFPDTSHGLRNLYFLDPIWLSECLQRIFNIKGSRSVAKNGVIRAEDLRMLLVGTGFTQQTEEQYFQFLAKFEIALPVANDSYLLPHLLPSKPGLDTHGMRHPTANTIQRVFKMSFVPVGFWQRFIARMLISLAEMDLQLFENKKNTKSRNRKVTIYSFTGNQRNRCSTFRVKRNQTIYWQEGLLVTFDGGYLSVESSDVNWKKKKSGGMKIVCQSEVRDFSAMAFITDHVNSLIDQWFPALTATESDGTPLMEQYVPCPVCETAWAQHTDPSEKSEDVQYFDMEDCVLTAIERDFISCPRHPDLPVPLQELVPELFMTDFPARLFLENSKLEHSEDEGSVLGQGGSGTVIYRARYQGQPVAVKRFHIKKFKNFANVPADTMLRHLRATDAMKNFSEFRQEASMLHALQHPCIVALIGISIHPLCFALELAPLSSLNTVLSENARDSSFIPLGHMLTQKIAYQIASGLAYLHKKNIIFCDLKSDNILVWSLDVKEHINIKLSDFGISRQSFHEGALGVEGTPGYQAPEIRPRIVYDEKVDMFSYGMVLYELLSGQRPALGHHQLQIAKKLSKGIRPVLGQPEEVQFRRLQALMMECWDTKPEKRPLALSVVSQMKDPTFATFMYELCCGKQTAFFSSQGQEYTVVFWDGKEESRNYTVVNTEKGLMEVQRMCCPGMKVSCQLQVQRSLWTATEDQKIYIYTLKGMCPLNTPQQALDTPAVVTCFLAVPVIKKNSYLVLAGLADGLVAVFPVVRGTPKDSCSYLCSHTANRSKFSIADEDARQNPYPVKAMEVVNSGSEVWYSNGPGLLVIDCASLEICRRLEPYMAPSMVTSVVCSSEGRGEEVVWCLDDKANSLVMYHSTTYQLCARYFCGVPSPLRDMFPVRPLDTEPPAASHTANPKVPEGDSIADVSIMYSEELGTQILIHQESLTDYCSMSSYSSSPPRQAARSPSSLPSSPASSSSVPFSTDCEDSDMLHTPGAASDRSEHDLTPMDGETFSQHLQAVKILAVRDLIWVPRRGGDVIVIGLEKDSGAQRGRVIAVLKARELTPHGVLVDAAVVAKDTVVCTFENENTEWCLAVWRGWGAREFDIFYQSYEELGRLEACTRKRR*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU68156
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Grow at or below 30ºC
• Low Temp: Yes