cDNA Clone NOTCH1

• Synonyms: hN1; AOS5; TAN1; AOVD1
• Plasmid: pCMV5D GFP NOTCH1 1754-end P2514A
• Parent Plasmid: pCMV5D GFP
• Expressed: GFP NOTCH1 1754-end P2514A
• Genbank: NM_017617.5 - P46531 (codon optimised)
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCATGGTGCTGCTGAGCCGGAAGAGAAGAAGGCAGCACGGCCAGCTGTGGTTCCCCGAGGGCTTTAAGGTGTCCGAGGCCAGCAAGAAGAAGCGGAGAGAGCCCCTGGGCGAGGATAGCGTGGGACTGAAGCCCCTGAAGAACGCCTCTGACGGCGCCCTGATGGACGACAACCAGAACGAATGGGGCGACGAGGACCTGGAAACAAAGAAGTTCAGATTCGAGGAACCCGTGGTGCTGCCCGACCTGGACGATCAGACCGACCACAGACAGTGGACCCAGCAGCATCTGGACGCCGCCGACCTGAGAATGTCTGCCATGGCCCCTACACCCCCTCAGGGCGAAGTGGACGCCGACTGCATGGATGTGAACGTGCGGGGACCCGACGGCTTCACCCCTCTGATGATCGCCAGCTGTTCTGGCGGCGGACTGGAAACCGGCAACAGCGAGGAAGAAGAGGACGCCCCTGCCGTGATCAGCGACTTCATATATCAGGGGGCCAGCCTGCACAACCAGACCGACAGAACAGGCGAGACAGCCCTGCATCTGGCCGCTCGGTACAGCAGATCTGATGCCGCCAAGCGGCTGCTGGAAGCCAGCGCCGATGCCAACATCCAGGACAACATGGGCAGAACCCCTCTGCATGCCGCCGTGTCTGCTGATGCTCAGGGCGTGTTCCAGATCCTGATCCGGAACAGAGCCACCGATCTGGATGCCCGGATGCACGATGGCACCACCCCACTGATTCTGGCCGCCAGACTGGCCGTGGAAGGCATGCTGGAAGATCTGATCAACAGCCACGCCGACGTGAACGCCGTGGACGATCTGGGAAAGAGCGCCCTGCATTGGGCAGCCGCCGTGAACAATGTGGACGCTGCTGTGGTGCTGCTGAAGAACGGCGCCAACAAGGACATGCAGAACAACAGAGAGGAAACCCCCCTGTTCCTGGCCGCTAGAGAGGGCAGCTACGAAACAGCCAAAGTGCTGCTGGATCACTTCGCCAACCGGGACATCACCGATCACATGGACAGACTGCCCCGGGATATCGCCCAGGAACGGATGCACCACGACATCGTGCGCCTGCTGGACGAGTACAACCTCGTGCGCTCTCCCCAGCTGCATGGCGCTCCTCTGGGAGGCACACCTACACTGAGCCCTCCACTGTGCAGCCCCAATGGCTACCTGGGCTCTCTGAAGCCTGGCGTGCAGGGCAAGAAAGTGCGGAAGCCTAGCAGCAAGGGCCTGGCCTGTGGCAGCAAAGAGGCCAAGGACCTGAAGGCCAGACGGAAGAAGTCCCAGGACGGCAAGGGATGTCTGCTGGACAGCAGCGGCATGCTGAGCCCCGTGGATAGCCTGGAAAGCCCTCACGGCTACCTGAGCGACGTGGCCTCTCCACCTCTGCTGCCTAGCCCTTTCCAGCAGAGCCCTAGCGTGCCCCTGAACCATCTGCCCGGCATGCCTGATACCCACCTGGGAATCGGCCACCTGAACGTGGCCGCCAAACCTGAAATGGCCGCACTGGGAGGCGGAGGCAGACTGGCTTTTGAGACAGGCCCCCCTCGGCTGTCCCATCTGCCAGTGGCAAGCGGCACAAGCACAGTGCTGGGCTCTAGCTCTGGCGGAGCCCTGAATTTCACAGTGGGCGGCAGCACCTCCCTGAACGGACAGTGTGAATGGCTGAGCAGACTGCAGAGCGGCATGGTGCCCAACCAGTACAACCCCCTGAGAGGCTCTGTGGCCCCTGGCCCACTGTCTACACAGGCCCCATCTCTGCAGCACGGAATGGTGGGACCTCTGCACAGCAGCCTGGCTGCCTCTGCCCTGAGCCAGATGATGAGCTACCAGGGCCTGCCCAGCACAAGACTGGCCACACAGCCACACCTGGTGCAGACACAGCAGGTGCAGCCTCAGAACCTGCAGATGCAGCAGCAGAATCTGCAGCCCGCCAACATTCAACAGCAGCAGTCCCTGCAGCCCCCTCCACCTCCTCCACAGCCTCATCTGGGAGTGTCTAGCGCCGCCTCTGGCCACCTGGGCAGAAGCTTTCTGAGCGGCGAACCCTCCCAGGCCGATGTGCAGCCACTGGGACCATCTTCCCTGGCTGTGCACACCATCCTGCCTCAGGAAAGCCCAGCCCTGCCTACCAGCCTGCCTAGCTCTCTGGTGCCTCCTGTGACAGCCGCCCAGTTCCTGACACCTCCTAGCCAGCACAGCTACAGCTCCCCCGTGGACAATACCCCCAGCCACCAGCTGCAGGTGCCCGAGCACCCATTTCTGACCCCTAGCGCCGAGAGCCCCGATCAGTGGTCTAGCAGCAGCCCCCACAGCAACGTGTCCGATTGGAGCGAAGGCGTGTCCAGCCCCCCAACCAGCATGCAGTCTCAGATCGCCAGAATCCCCGAGGCCTTCAAGTAGGCGGCCGC

Amino Acid Sequence

MHVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGSAMVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDN
QNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAV
ISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEG
MLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDI
VRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGY
LSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCE
WLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQ
PPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPF
LTPSAESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCMV5D GFP NOTCH1 1754-end P2514A67.63 KB Snapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61220
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: NICD (Notch intracellular domain). Codon optimised sequence. All NOTCH1 plasmids MUST be grown at 30C or less to prevent recombination.
• Low Temp: No