cDNA Clone Srpk2 (mouse)

• Synonyms: Wbp6; mSRPK2; AW226533; AW492537; AW547358
• Plasmid: pCAGGS puro HA mouse Srpk2 D523A
• Parent Plasmid: pCAGGS puro HA
• Expressed: HA mouse Srpk2 D523A
• Genbank: GI: 20817, NP_033300.2 with delta Q314 - common variant, and some silent changes
• Tag: HA
• Species: Mouse

Sequence of Insert

GGATCCATGTCAGTTAACTCTGAGAAGTCGTCCTCTTCAGAAAGGCCGGAGCCTCAACAGAAAGCTCCTTTAGTTCCTCCTCCTCCACCACCACCACCGCCACCACCACTGCCAGACCCCGCGCCCCCAGAGCCAGAGGAGGAGATTCTGGGGTCAGATGATGAGGAGCAGGAGGACCCCGCAGATTACTGCAAAGGTGGCTATCATCCAGTGAAAATTGGAGATCTCTTCAATGGTCGATATCATGTCATTAGAAAGCTAGGATGGGGGCACTTTTCTACTGTATGGCTGTGCTGGGATATGCAAGGGAAAAGATTTGTTGCAATGAAAGTTGTAAAAAGTGCCCAGCATTATACAGAGACAGCCTTGGATGAAATTAAACTACTCAAATGCGTTCGAGAAAGTGACCCCAGTGACCCAAACAAAGACATGGTAGTTCAGCTAATTGATGACTTCAAGATCTCAGGCATGAATGGGATACATGTCTGCATGGTCTTTGAAGTACTTGGTCACCATCTCCTCAAATGGATCATCAAATCCAACTATCAAGGCCTCCCAGTACGTTGTGTGAAGAGTATCATTCGACAGGTCCTTCAAGGGTTAGATTATCTACACAGTAAGTGCAAGATAATTCACACCGACATAAAGCCGGAAAACATCTTGATGTGTGTGGATGACGCTTACGTGAGAAGAATGGCAGCCGAAGCCACGGAGTGGCAGAAAGCAGGTGCTCCTCCTCCCTCTGGGTCTGCAGTGAGTACGGCTCCACAGCAAAAACCTATAGGAAAAATATCTAAAAACAAAAAGAAAAAGCTGAAAAAGAAACAGAAGAGACAGGCTGAGTTGCTGGAGAAACGCCTACAGGAGATTGAGGAATTGGAGCGAGAAGCCGAAAGGAAAATCCTAGAGGAGAACATCACCTCTGCAGAAGCTTCCGGGGAGCAGGATGGAGAGTACCAGCCGGAGGTGACACTGAAAGCAGCCGACTTAGAGGACACAACTGAGGAAGAGACAGCAAAGGATAATGGTGAAGTTGAAGACCAGGAAGAGAAAGAAGATGCGGAGAAGGAGAACGCGGAGAAGGATGAAGATGATGTTGAACAGGAACTTGCAAACTTAGACCCGACCTGGGTGGAGTCCCCGAAAGCCAATGGCCATATTGAAAATGGCCCGTTCTCACTGGAGCAGCAGCTGGAGGATGAAGAGGACGATGAAGATGACTGTGCAAATCCCGAGGAGTATAACCTCGATGAGCCAAATGCAGAGAGTGATTACACGTATAGCAGCTCCTATGAACAATTCAATGGTGAATTGCCAAATGGACAACATAAGACTTCAGAGTTTCCCACACCGTTGTTTTCTGGGCCCTTAGAACCTGTGGCCTGTGGCTCTGTGATTTCAGAGGGATCGCCACTTACCGAGCAGGAGGAAAGCAGTCCCTCCCATGACAGAAGCAGGACAGTTTCAGCCTCTAGTACTGGAGATTTGCCAAAAACAAAAACCCGGGCGGCTGACCTGTTGGTGAACCCTCTTGATCCACGGAATGCAGATAAAATTAGAGTAAAAATTGCTGCTCTGGGAAATGCTTGTTGGGTGCATAAACATTTCACAGAGGATATCCAGACACGTCAGTATAGGTCCATAGAGGTTTTGATAGGAGCAGGCTACAGCACACCTGCAGATATTTGGAGTACAGCTTGCATGGCATTTGAGCTCGCCACAGGAGACTATTTGTTCGAACCGCATTCTGGGGAAGACTATTCCAGAGATGAAGACCACATAGCCCACATCATAGAGCTGCTAGGCAGTATCCCAAGGCACTTTGCTCTGTCTGGAAAATATTCTCGGGAATTCTTCAATCGCAGAGGAGAACTGCGGCACATCACCAAGCTGAAGCCCTGGAGCCTCTTTGATGTACTTGTGGAAAAGTATGGCTGGCCCCATGAAGATGCTGCACAATTTACAGATTTCCTGATCCCAATGTTAGAGATGGTTCCAGAAAAACGAGCCTCAGCTGGCGAATGCCTTCGACATCCTTGGTTGAATTCTTAGCGGCCGC

Amino Acid Sequence

MYPYDVPDYAGSMSVNSEKSSSSERPEPQQKAPLVPPPPPPPPPPPLPDPAPPEPEEEILGSDDEEQEDPADYCKGGYHPVKIGDLFNGRYHVIRKLGWG
HFSTVWLCWDMQGKRFVAMKVVKSAQHYTETALDEIKLLKCVRESDPSDPNKDMVVQLIDDFKISGMNGIHVCMVFEVLGHHLLKWIIKSNYQGLPVRCV
KSIIRQVLQGLDYLHSKCKIIHTDIKPENILMCVDDAYVRRMAAEATEWQKAGAPPPSGSAVSTAPQQKPIGKISKNKKKKLKKKQKRQAELLEKRLQEI
EELEREAERKILEENITSAEASGEQDGEYQPEVTLKAADLEDTTEEETAKDNGEVEDQEEKEDAEKENAEKDEDDVEQELANLDPTWVESPKANGHIENG
PFSLEQQLEDEEDDEDDCANPEEYNLDEPNAESDYTYSSSYEQFNGELPNGQHKTSEFPTPLFSGPLEPVACGSVISEGSPLTEQEESSPSHDRSRTVSA
SSTGDLPKTKTRAADLLVNPLDPRNADKIRVKIAALGNACWVHKHFTEDIQTRQYRSIEVLIGAGYSTPADIWSTACMAFELATGDYLFEPHSGEDYSRD
EDHIAHIIELLGSIPRHFALSGKYSREFFNRRGELRHITKLKPWSLFDVLVEKYGWPHEDAAQFTDFLIPMLEMVPEKRASAGECLRHPWLNS*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pCAGGS puro HA mouse Srpk2 D523A453.64 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU61817
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Kinase Dead
• Low Temp: No