cDNA Clone (mu) MYCBP2

• Plasmid: pSc-B-(mu)MYCBP2 nt10107-end
• Parent Plasmid: pSC-B
• Expressed: Mouse Mycbp2
• Genbank: NM_207215.2 → NP_997098.2
• Species: Mouse

Sequence of Insert

GGTACCTCGTATGCGATCGCTGTAGAGAAAAATACCTCCGTGAAAAACAG
GCCGCTGCAAGGGAAAAGGTCAAACAATCTAGAAGAAAGCCAATGCAAGT
AAAGACGCCTCGTGCCTTGCCCACCATGGAAGCACACCAGGTGATCAAAG
CCAACGCCCTCTTCCTGCTGTCCCTGAGCAGTGCTGCAGAGCCAAGCATC
TTGTGTTACCACCCTGCAAAGCCATTCCAGTCTCAGCTGCCCATCGTGAA
GGAAGGCGTTTCTGAGGACCTTCCTGTAAAAATGCCTTGTCTCTACTTGC
AGACATTAGCTAGGCACCACCATGAAAATTTTGTGGGCTATCAAGACGAC
AACCTATTCCAGGACGAAATGAGATATCTGCGTTCTACATCTGTCCCTGC
CCCATACATATCAGTAACTCCTGATGCAAGTCCCAATGTATTTGAAGAGC
CAGAGAGCAATATGAAGTCTATGCCACCAAGTTTGGAAACTAGCCCCATA
ACCGACACTGACCTGGCTAAGAGAACTGTCTTCCAAAGGTCATACTCAGT
TGTTGCTTCTGAGTATGATAAACAACATTCCATTTTACCTGCACGAGTTA
AAGCCATCCCTAGAAGAAGAGTGAACAGTGGAGACACTGAAGTTGGGTCT
TCCCTCTTGCGACATCCATCGCCAGAGCTTTCCCGGCTCATATCAGCCCA
CAGCTCTCTTTCCAAAGGAGAACGGAACTTCCAGTGGCCAGTTTTAGCTT
TTGTTATACAGCATCATGATTTAGAAGGGCTTGAAATAGCAATGAAGCAG
GCCTTAAGGAAGTCTGCTTGCCGTGTGTTTGCAATGGAGGCATTCAACTG
GCTTCTCTGTAATGTCATCCAAACAACATCTCTGCATGACATTCTCTGGC
ACTTTGTGGCTGCACTGACGCCTTCTCCCGTAGAAGCAGAGGAAGATGAG
GATGAAGATAATAAATCCAATAAAGAAAATGCAGAGCAAGAGAAGGATAC
AAGAGTGTGTGAGCATCCACTGTCAGATATAGTGATTGCAGGCGAAGCTG
CTCACCCACTGCCACATACGTTTCACCGGTTACTTCAGACCATCTCTGAC
CTTATGATGTCCCTCCCTAGTGGCAGTTCATTGCAACAAATGGCCTTAAG
ATGCTGGAGCCTCAAGTTCAAGCAGTCCGATCACCAGTTCCTCCATCAGA
GCAATGTCTTCCATCACATCAACAACATCTTGTCGAAGTCAGACGATGGA
GACAGTGAGGAGAGCTTCAGCATCAGCGTCCAGTCTGGCTTTGAAGCTAT
GAGCCAGGAATTATGCATAGTTATGTGCCTAAAGGACTTGACTAGCATCG
TTGACATAAAAACATCAAGTCGACCCGCCATGATTGGCAGTTTGACAGAT
GGCTCCACAGAAACCTTTTGGGAATCAGGAGATGAAGATAAAAACAAAAC
TAAAAATATCACTATCAACTGTGTGAAGGGAATTAATGCCCGTTATGTAT
CTGTTCATGTGGACAACTCCCGGGATCTGGGGAATAAAGTTACCTCAATG
ACCTTCTTAACTGGGAAAGCAGTAGAAGAGCTGTGCAGAATAAAGCAGGT
CGATTTGGATTCCAGGCACATTGGCTGGGTCACAAGTGAACTCCCAGGAG
GGGATAATCAGATCATCAAAATAGAGTTAAAGGGCCCAGAAAATACATTA
AGAGTTCGACAAGTAAAAGTCCTGGGTTGGAAAGATGGTGAAAGCACAAA
AATCGCTGGCCAGATCTCAGCCAGTGTGGCTCAGCAAAGGAGCTGTGAGG
CTGAGACTCTCCGAGTGTTCAGACTCATCACCTCTCAAGTGTTTGGAAAG
CTCATCTCTGGAGACGCTGAACCCACACCAGAGCAAGAGGAAAAGGCGCT
CCTGTCCTCCCCTGAAGGAGAAGAAAAAGTATACAATGCGACATCTGATG
CTGACCTGAAGGAACACATGGTTGGAATCATATTCAGCAGGAGTAAACTG
ACTAACCTACAAAAGCAGGTGTGTGCACACATCGTCCAAGCTATTCGCAT
GGAAGCCACAAGAGTCCGGGAAGAATGGGAACACGCCATATCCAGCAAGG
AGAACGCGAACTCCCAGCCCAGTGATGAGGACGCCTCCTCTGACGCTTAC
TGCTTCGAGCTGCTCTCTATGGTCCTAGCGCTGAGCGGCTCCAACGTTGG
CAGACAGTACCTGGCCCAGCAGCTGACTCTGCTGCAGGACCTGTTCTCCT
TGCTCCACACAGCCTCCCCGAGGGTGCAGAGACAGGTGACCTCCTTACTA
AGGCGAGTTTTGCCTGAAGTGACTCCTAACCGTCTGGCCAGTATCATCGG
AGTGAAGTCCCTCCCCCCAGCTGACATCAGTGACATCATTCACTCAACTG
AGAAAGGAGACTGGAATAAGCTGGGTATCTTGGACATGTTTCTGGGATGC
ATTGCCAAGGCGCTCACTGTCCAGCTCAAAGCCAAAGGGACCACCATCAC
TGGGACAGCCGGCACCACTGTGGGCAAAGGAGTTACAACAGTCACCCTGC
CAATGATTTTCAACTCCAGTTACCTCCGACGAGGTGAGAGTCACTGGTGG
ATGAAGGGCTCAACTCCTACTCAGATCTCAGAGATCATCATCAGGCTTAT
CAAGGATATGGCTGCAGGTCACCTGTCAGAAGCTTGGTCCCGAGTGACAA
AAAATGCTATTGCAGAAACCATCATTGCCTTGACCAAAATGGAAGAAGAA
TTTAGGTCTCCAGTGAGATGTATTGCAACAACTAGACTCTGGCTTGCCCT
GGCATCGCTGTGTGTTCTTGATCAGGACCATGTGGACCGCCTCTCCTCGG
GGAGATGGATGGGAAAGGACGGGCAACAGAAGCAAATGCCTATGTGTGAC
AACCATGATGACGGTGAAACTGCAGCTATCATCTTATGCAACATCTGTGG
CAATCTGTGTACTGACTGTGACAGATTCCTTCATCTGCATCGACGAACGA
AGACTCACCAAAGACAGGTCTTCAAAGAAGAAGAAGAGGCTATAAAGGTC
GACCTCCATGAAGGCTGTGGTAGAACAAAACTGTTCTGGTTGATGGCATT
AGCAGATTCTAAAACAATGAAGGCAATGGTGGAATTCCGAGAACACACAG
GCAAACCCACCACGAGTAGCTCGGAAGCATGCCGCTTCTGTGGGTCCAGG
AGTGGAACGGAGCTGTCTGCTGTTGGCAGTGTTTGTTCTGATGCAGACTG
CCAGGAATATGCTAAGATCGCCTGCAGTAAGACACATCCCTGCGGACATC
CGTGTGGAGGTGTTAGAAACGAAGAGCACTGCCTGCCCTGCCTGCACGGC
TGTGATAAGAGCGCCACCACACTGAAACAAGATGCCGACGACATGTGCAT
GATCTGCTTCACCGAAGCGCTCTCTGCAGCACCGGCCATTCAGTTGGACT
GCAGTCATGTGTTCCACTTACAGTGCTGTCGGCGAGTTTTGGAAAACAGA
TGGCTTGGTCCAAGGATAACGTTTGGATTCATTTCGTGTCCTATTTGCAA
GAACAAGATAAATCATATAGTATTAAAAGACCTGCTTGATCCAATAAAAG
AGCTCTATGAAGACGTCAGAAGAAAAGCCTTAATGAGATTGGAGTATGAA
GGTCTGCATAAGAGTGAAGCTATCACGACTCCTGGTGTCAGGTTTTATAA
TGATGCAGCTGGCTATGCCATGAATAGATACGCATATTATGTCTGCTACA
AGTGCAGAAAGGCATACTTTGGTGGTGAGGCTCGCTGTGATGCTGAGGCT
GGACAAGGAGATGATTACGACCCCAGAGAGCTCATCTGCGGCGCCTGTTC
CGATGTGTCCAGGGCCCAGATGTGTCCCAAACATGGAACAGACTTTCTAG
AATACAAGTGTCGCTACTGCTGTTCGGTGGCTGTCTTCTTCTGTTTCGGA
ACAACACATTTCTGTAATGCTTGTCATGATGACTTTCAGAGAATGACCAG
CATTCCTAAGGAAGAACTCCCACACTGTCCTGCAGGTCCCAAAGGCAAGC
AGCTAGAAGGAACTGAGTGTCCACTCCACGTTGTTCATCCGCCCACGGGG
GAAGAGTTTGCTCTCGGGTGCGGCGTGTGCAGAAATGCTCACACGTTTTA
Ggcggccgc

Amino Acid Sequence

YLVCDRCREKYLREKQAAAREKVKQSRRKPMQVKTPRALPTMEAHQVIKA
NALFLLSLSSAAEPSILCYHPAKPFQSQLPIVKEGVSEDLPVKMPCLYLQ
TLARHHHENFVGYQDDNLFQDEMRYLRSTSVPAPYISVTPDASPNVFEEP
ESNMKSMPPSLETSPITDTDLAKRTVFQRSYSVVASEYDKQHSILPARVK
AIPRRRVNSGDTEVGSSLLRHPSPELSRLISAHSSLSKGERNFQWPVLAF
VIQHHDLEGLEIAMKQALRKSACRVFAMEAFNWLLCNVIQTTSLHDILWH
FVAALTPSPVEAEEDEDEDNKSNKENAEQEKDTRVCEHPLSDIVIAGEAA
HPLPHTFHRLLQTISDLMMSLPSGSSLQQMALRCWSLKFKQSDHQFLHQS
NVFHHINNILSKSDDGDSEESFSISVQSGFEAMSQELCIVMCLKDLTSIV
DIKTSSRPAMIGSLTDGSTETFWESGDEDKNKTKNITINCVKGINARYVS
VHVDNSRDLGNKVTSMTFLTGKAVEELCRIKQVDLDSRHIGWVTSELPGG
DNQIIKIELKGPENTLRVRQVKVLGWKDGESTKIAGQISASVAQQRSCEA
ETLRVFRLITSQVFGKLISGDAEPTPEQEEKALLSSPEGEEKVYNATSDA
DLKEHMVGIIFSRSKLTNLQKQVCAHIVQAIRMEATRVREEWEHAISSKE
NANSQPSDEDASSDAYCFELLSMVLALSGSNVGRQYLAQQLTLLQDLFSL
LHTASPRVQRQVTSLLRRVLPEVTPNRLASIIGVKSLPPADISDIIHSTE
KGDWNKLGILDMFLGCIAKALTVQLKAKGTTITGTAGTTVGKGVTTVTLP
MIFNSSYLRRGESHWWMKGSTPTQISEIIIRLIKDMAAGHLSEAWSRVTK
NAIAETIIALTKMEEEFRSPVRCIATTRLWLALASLCVLDQDHVDRLSSG
RWMGKDGQQKQMPMCDNHDDGETAAIILCNICGNLCTDCDRFLHLHRRTK
THQRQVFKEEEEAIKVDLHEGCGRTKLFWLMALADSKTMKAMVEFREHTG
KPTTSSSEACRFCGSRSGTELSAVGSVCSDADCQEYAKIACSKTHPCGHP
CGGVRNEEHCLPCLHGCDKSATTLKQDADDMCMICFTEALSAAPAIQLDC
SHVFHLQCCRRVLENRWLGPRITFGFISCPICKNKINHIVLKDLLDPIKE
LYEDVRRKALMRLEYEGLHKSEAITTPGVRFYNDAAGYAMNRYAYYVCYK
CRKAYFGGEARCDAEAGQGDDYDPRELICGACSDVSRAQMCPKHGTDFLE
YKCRYCCSVAVFFCFGTTHFCNACHDDFQRMTSIPKEELPHCPAGPKGKQ
LEGTECPLHVVHPPTGEEFALGCGVCRNAHTF*

• Vector Type: Other
• Antibiotic: Amp; Kan
• DU Number: DU65486
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: From native Kpn1 site at nt10,107 to end (incl. stop & Not1 site ); comprises Tyr3369-Phe4746 (end) of full-length protein.; Clone contains a single residue (Glu) insertion between T3580 and V3581 and a triple residue insertion (Val-Tyr-Asn) between K4009 and Ala4010 compared to NCBI reference
• Low Temp: No