cDNA Clone TRIM27

• Plasmid: pGEX6P-1-TRIM27 c-HA
• Parent Plasmid: pGEX6P1
• Expressed: GST-TRIM27-HA
• Genbank: NP_006501.1
• Tag: GST
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCCTTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTACCCATACGATGTGCCAGATTACGCCTAGGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSPypydvpdya*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU19017
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid to express TRIM27 with N-terminal GST and c-terminal HA tags.