cDNA Clone TRIM27

• Plasmid: pMal-6His TRIM27
• Parent Plasmid: pMAL6P
• Expressed: MBP.6His-TRIM27
• Tag: MBP
• Species: Human

Sequence of Insert

GGATCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCAC
CTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCG
GCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAG
ACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACAT
GCGGCCCAACCGGCACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGC
GCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGAGAAG
CACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTG
CGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGC
TCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGAC
CATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGA
ACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGA
TTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGAGTAT
CGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCAT
CAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCC
TGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTG
CAGGACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGA
ACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCC
AAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATG
CAGTCAGATATGGAGAAAATCCAAGAATTAAGAGAGGCTCAGTTATACTC
AGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTGATCCTCT
CTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCT
GACAACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATG
CTTCATCGCCGGGAGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCA
AGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGGAGTA
ACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAA
AGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTGCGGACCC
CGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCC
TTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTAC
CTTTTGTGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGA
AAAGTGCAGCTCCTCTGATCATCTGCCCCATGAGTGGGATAGATGGGTTT
TCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCTTGAGC
GGCCGC

Amino Acid Sequence

MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFG
GYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYN
KDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYA
FKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFN
KGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINA
ASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIA
ATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNS
SSNNNNNNNNNNLGLEVLFQGPLGSMSYYHHHHHHDYDIPTTENLYFQGA
MGSMASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTA
ETNVSCPQCRETFPQRHMRPNRHLANVTQLVKQLRTERPSGPGGEMGVCE
KHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFKEQIQNQL
DHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHE
YRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTREL
LQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEK
MQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDL
PDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGG
VTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEV
SFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDG
FSGHVGNHGHSMETSP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU20459
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct for bacterial expression of N-terminally MBP.6HIS tagged TRIM27 (MBP-Precission-6HIS-TEV-TRIM27); ; Estimated MW of expressed protein : 107561 Daltons