cDNA Clone TRIM49

• Synonyms: RNF18, TRIM49L2
• Plasmid: pMK-RQ TRIM49 Cter GFP donor
• Parent Plasmid: pMK-RQ
• Expressed: TRIM49-GFP (following recombination)
• Genbank: ENSG00000168930
• Species: Human

Sequence of Insert

TTTTTTTCTTATTTACACATGTCTATGCATGTTTTCTTTTTTTTTTTTTTTGCAGTGCATATTACTCTGCATCATGAAGAAGCCAACAATGATATCTTTCTGTATGAAATTTTGAGAAGCATGTGTATTGGATGTGACCATCAAGATGTACCCTATTTCACTGCAACACCTAGAAGTTTTCTTGCATGGGGTGTTCAGACTTTCACCTCGGGCAAATATTACTGGGAGGTCCATGTAGGGGACTCCTGGAATTGGGCTTTTGGTGTCTGTAATATGTATCGGAAAGAGAAGAATCAGAATGAGAAGATAGATGGAAAGGCGGGACTCTTTCTTCTTGGGTGTGTTAAGAATGACATTCAATGCAGTCTCTTTACCACCTCCCCACTTATGCTGCAATATATCCCAAAACCTACCAGCCGAGTAGGATTATTCCTGGATTGTGAGGCTAAGACTGTGAGCTTTGTTGATGTTAATCAAAGCTCCCTAATATACACCATCCCgAAcTGtagcTTtagcCCaCCTCTCAGGCCTATCTTTTGCTGTATTCACTTCGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGtgaCCAGAGACAAATCAGAAATGTGTTCACATGCTGTGGGAACCCCTTTATCCCAGGAAGTCCTCTTCCTTGTGCCTTAACATACAGGACAAATAGGCTCTATTTTATGTCTTGAATTGCCTTCTAATGTTATCAAAACTCATTTATTGTGTTACTATTAAATATGCTGAAAACGCTAAAAGTATACGTATTGGTTCTTTATTAAATAATTTTTGAAAAATCATTATTCATGATCATGGCATACAGTATATTCTCTTTTTTTTCTTTATTTATGACTGTCACTGAGTGAAATAATAGATGACAGACATGTCTGAATGAAGTAAAAATCAATGGAAGACAGTCGGGATCTTTTGCTTCATGCAAAAAACTTGGAGTGAAGTCTCAATGATAACTGGGAAATGTTTTTCTTCCTCTTTATCTAACTATATTACACTTATCCATCAGGTTTCATTGTATTAATCTATCCTTTGAGGTAATACTATTTGACCTTCTATGCTGGG

Amino Acid Sequence

MNSGILQVFQGELICPLCMNYFIDPVTIDCGHSFCRPCFYLNWQDIPFLV
QCSECTKSTEQINLKTNIHLKKMASLARKVSLWLFLSSEEQMCGTHRETK
KIFCEVDRSLLCLLCSSSQEHRYHRHRPIEWAAEEHREKLLQKMQSLWEK
ACENHRNLNVETTRTRCWKDYVNLRLEAIRAEYQKMPAFHHEEEKHNLEM
LKKKGKEIFHRLHLSKAKMAHRMEILRGMYEELNEMCHKPDVELLQAFGD
ILHRSESVLLHMPQPLNPELSAGPITGLRDRLNQFRVHITLHHEEANNDI
FLYEILRSMCIGCDHQDVPYFTATPRSFLAWGVQTFTSGKYYWEVHVGDS
WNWAFGVCNMYRKEKNQNEKIDGKAGLFLLGCVKNDIQCSLFTTSPLMLQ
YIPKPTSRVGLFLDCEAKTVSFVDVNQSSLIYTIPNCSFSPPLRPIFCCI
HFVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFIC
TTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTI
FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH
NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN
HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*

• Vector Type: Other
• Antibiotic: Kan
• DU Number: DU48191
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: CRISPR donor designed to insert an in-frame GFP to the c-terminal end of TRIM49 (exon 8) via homologous recombination. Consists of the reporter flanked on either side by 500 bp of TRIM49 homology and is to be co-transfected with 48097 into a Cas9 expressing cell-line.; Sequence of insert shows the donor sequence whilst sequence of cassette and amino acid sequence depict the expected expression product from the resultant transcript.