cDNA Clone SRPK1

• Plasmid: pGEX6P-1 SRPK1 D497A
• Parent Plasmid: pGEX6P-1
• Expressed: GST SRPK1 D497A
• Genbank: NM_003137, NP_003128.3
• Tag: GST

Sequence of Insert

GGATCCGAGCGGAAAGTGCTTGCGCTCCAGGCCCGAAAGAAAAGGACCAAGGCCAAGAAGGACAAAGCCCAAAGGAAATCTGAAACTCAGCACCGAGGCTCTGCTCCCCACTCTGAGAGTGATCTACCAGAGCAGGAAGAGGAGATTCTGGGATCTGATGATGATGAGCAAGAAGATCCTAATGATTATTGTAAAGGAGGTTATCATCTTGTGAAAATTGGAGATCTATTCAATGGGAGATACCATGTGATCCGAAAGTTAGGCTGGGGACACTTTTCAACAGTATGGTTATCATGGGATATTCAGGGGAAGAAATTTGTGGCAATGAAAGTAGTTAAAAGTGCTGAACATTACACTGAAACAGCACTAGATGAAATCCGGTTGCTGAAGTCAGTTCGCAATTCAGACCCTAATGATCCAAATAGAGAAATGGTTGTTCAACTACTAGATGACTTTAAAATATCAGGAGTTAATGGAACACATATCTGCATGGTATTTGAAGTTTTGGGGCATCATCTGCTCAAGTGGATCATCAAATCCAATTATCAGGGGCTTCCACTGCCTTGTGTCAAAAAAATTATTCAGCAAGTGTTACAGGGTCTTGATTATTTACATACCAAGTGCCGTATCATCCACACTGACATTAAACCAGAGAACATCTTATTGTCAGTGAATGAGCAGTACATTCGGAGGCTGGCTGCAGAAGCAACAGAATGGCAGCGATCTGGAGCTCCTCCGCCTTCCGGATCTGCAGTCAGTACTGCTCCCCAGCCTAAACCAGCTGACAAAATGTCAAAGAATAAGAAGAAGAAATTGAAGAAGAAGCAGAAGCGCCAGGCAGAATTACTAGAGAAGCGAATGCAGGAAATTGAGGAAATGGAGAAAGAGTCGGGCCCTGGGCAAAAAAGACCAAACAAGCAAGAAGAATCAGAGAGTCCTGTTGAAAGACCCTTGAAAGAGAACCCACCTAATAAAATGACCCAAGAAAAACTTGAAG
AGTCAAGTACCATTGGCCAGGATCAAACGCTTATGGAACGTGATACAGAGGGTGGTGCAGCAGAAATTAATTGCAATGGAGTGATTGAAGTCATTAATTATACTCAGAACAGTAATAATGAAACATTGAGACATAAAGAGGATCTACATAATGCTAATGACTGTGATGTCCAAAATTTGAATCAGGAATCTAGTTTCCTAAGCTCCCAAAATGGAGACAGCAGCACATCTCAAGAAACAGACTCTTGTACACCTATAACATCTGAGGTGTCAGACACCATGGTGTGCCAGTCTTCCTCAACTGTAGGTCAGTCATTCAGTGAACAACACATTAGCCAACTTCAAGAAAGCATTCGGGCAGAGATACCCTGTGAAGATGAACAAGAGCAAGAACATAACGGACCACTGGACAACAAAGGAAAATCCACGGCTGGAAATTTTCTTGTTAATCCCCTTGAGCCAAAAAATGCAGAAAAGCTCAAGGTGAAGATTGCTGCCCTTGGAAATGCTTGTTGGGTGCACAAACATTTCACTGAAGATATTCAAACAAGGCAATATCGTTCCTTGGAAGTTCTAATCGGATCTGGCTATAATACCCCTGCTGACATTTGGAGCACGGCATGCATGGCCTTTGAACTGGCCACAGGTGACTATTTGTTTGAACCTCATTCAGGGGAAGAGTACACTCGAGATGAAGATCACATTGCATTGATCATAGAACTTCTGGGGAAGGTGCCTCGCAAGCTCATTGTGGCAGGAAAATATTCCAAGGAATTTTTCACCAAAAAAGGTGACCTGAAACATATCACGAAGCTGAAACCTTGGGGCCTTTTTGAGGTTCTAGTGGAGAAGTATGAGTGGTCTCAGGAAGAGGCAGCTGGCTTCACAGATTTCTTACTGCCCATGTTGGAGCTGATCCCTGAGAAGAGAGCCACTGCCGCCGAGTGTCTCCGGCACCCTTGGCTTAACTCCTAAGAATTC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSERKVLALQARKKRTKAKKDKAQRKSETQHRGSAPHSESDLPEQEEEILGSDDDEQEDPNDYCKGGYHLVKIGDLFNGRYHVIRKLGWGHFSTVWLSWDIQGKKFVAMKVVKSAEHYTETALDEIRLLKSVRNSDPNDPNREMVVQLLDDFKISGVNGTHICMVFEVLGHHLLKWIIKSNYQGLPLPCVKKIIQQVLQGLDYLHTKCRIIHTDIKPENILLSVNEQYIRRLAAEATEWQRSGAPPPSGSAVSTAPQPKPADKMSKNKKKKLKKKQKRQAELLEKRMQEIEEMEKESGPGQKRPNKQEESESPVERPLKENPPNKMTQEKLEESSTIGQDQTLMERDTEGGAAEINCNGVIEVINYTQNSNNETLRHKEDLHNANDCDVQNLNQESSFLSSQNGDSSTSQETDSCTPITSEVSDTMVCQSSSTVGQSFSEQHISQLQESIRAEIPCEDEQEQEHNGPLDNKGKSTAGNFLVNPLEPKNAEKLKVKIAALGNACWVHKHFTEDIQTRQYRSLEVLIGSGYNTPADIWSTACMAFELATGDYLFEPHSGEEYTRDEDHIALIIELLGKVPRKLIVAGKYSKEFFTKKGDLKHITKLKPWGLFEVLVEKYEWSQEEAAGFTDFLLPMLELIPEKRATAAECLRHPWLNS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU66208
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Hi could you please mutate Asp 497 to Ala in GST human SRPK1 (DU 967). Thanks
• Low Temp: No