HOIL1

• Plasmid: pRetroX Tight FLAG-HOIL1
• Parent Plasmid: pRetroX Tight (puro) FLAG
• Expressed: FLAG-HOIL
• Genbank: NM_031229
• Tag: HA
• Species: Human

Sequence of Insert

GCGGCCGCGATGGACGAGAAGACCAAGAAAGCAGAGGAAATGGCCCTGAG
CCTCACCCGAGCAGTGGCGGGCGGGGATGAACAGGTGGCAATGAAGTGTG
CCATCTGGCTGGCAGAGCAACGGGTGCCCCTGAGTGTGCAACTGAAGCCT
GAGGTCTCCCCAACGCAGGACATCAGGCTGTGGGTGAGCGTGGAGGATGC
TCAGATGCACACCGTCACCATCTGGCTCACAGTGCGCCCTGATATGACAG
TGGCGTCTCTCAAGGACATGGTTTTTCTGGACTATGGCTTCCCACCAGTC
TTGCAGCAGTGGGTGATTGGGCAGCGGCTGGCACGAGACCAGGAGACCCT
GCACTCCCATGGGGTGCGGCAGAATGGGGACAGTGCCTACCTCTATCTGC
TGTCAGCCCGCAACACCTCCCTCAACCCTCAGGAGCTGCAGCGGGAGCGG
CAGCTGCGGATGCTGGAAGATCTGGGCTTCAAGGACCTCACGCTGCAGCC
GCGGGGCCCTCTGGAGCCAGGCCCCCCAAAGCCCGGGGTCCCCCAGGAAC
CCGGACGGGGGCAGCCAGATGCAGTGCCTGAGCCCCCACCGGTGGGCTGG
CAGTGCCCCGGGTGCACCTTCATCAACAAGCCCACGCGGCCTGGCTGTGA
GATGTGCTGCCGGGCGCGCCCCGAGGCCTACCAGGTCCCCGCCTCATACC
AGCCCGACGAGGAGGAGCGAGCGCGCCTGGCGGGCGAGGAGGAGGCGCTG
CGTCAGTACCAGCAGCGGAAGCAGCAGCAGCAGGAGGGGAACTACCTGCA
GCACGTCCAGCTGGACCAGAGGAGCCTGGTGCTGAACACGGAGCCCGCCG
AGTGCCCCGTGTGCTACTCGGTGCTGGCGCCCGGCGAGGCCGTGGTGCTG
CGTGAGTGTCTGCACACCTTCTGCAGGGAGTGCCTGCAGGGCACCATCCG
CAACAGCCAGGAGGCGGAGGTCTCCTGCCCCTTCATTGACAACACCTACT
CGTGCTCGGGCAAGCTGCTGGAGAGGGAGATCAAGGCGCTCCTGACCCCT
GAGGATTACCAGCGATTTCTAGACCTGGGCATCTCCATTGCTGAAAACCG
CAGTGCCTTCAGCTACCATTGCAAGACCCCAGATTGCAAGGGATGGTGCT
TCTTTGAGGATGATGTCAATGAGTTCACCTGCCCTGTGTGTTTCCACGTC
AACTGCCTGCTCTGCAAGGCCATCCATGAGCAGATGAACTGCAAGGAGTA
TCAGGAGGACCTGGCCCTGCGGGCTCAGAACGATGTGGCTGCCCGGCAGA
CGACAGAGATGCTGAAGGTGATGCTGCAGCAGGGCGAGGCCATGCGCTGC
CCCCAGTGCCAGATCGTGGTACAGAAGAAGGACGGCTGCGACTGGATCCG
CTGCACCGTCTGCCACACCGAGATCTGCTGGGTCACCAAGGGCCCACGCT
GGGGCCCTGGGGGCCCAGGAGACACCAGCGGGGGCTGCCGCTGCAGGGTA
AATGGGATTCCTTGCCACCCAAGCTGTCAGAACTGCCACTGAGCGGCCGC

Amino Acid Sequence

MDYKDDDDKGSAAAMDEKTKKAEEMALSLTRAVAGGDEQVAMKCAIWLAEQRVPLSVQLKPEVSPTQDIRLWVSVEDAQMHTVTIWLTVRPDMTVASLKDMVFLDYGFPPVLQQWVIGQRLARDQETLHSHGVRQNGDSAYLYLLSARNTSLNPQELQRERQLRMLEDLGFKDLTLQPRGPLEPGPPKPGVPQEPGRGQPDAVPEPPPVGWQCPGCTFINKPTRPGCEMCCRARPEAYQVPASYQPDEEERARLAGEEEALRQYQQRKQQQQEGNYLQHVQLDQRSLVLNTEPAECPVCYSVLAPGEAVVLRECLHTFCRECLQGTIRNSQEAEVSCPFIDNTYSCSGKLLEREIKALLTPEDYQRFLDLGISIAENRSAFSYHCKTPDCKGWCFFEDDVNEFTCPVCFHVNCLLCKAIHEQMNCKEYQEDLALRAQNDVAARQTTEMLKVMLQQGEAMRCPQCQIVVQKKDGCDWIRCTVCHTEICWVTKGPRWGPGGPGDTSGGCRCRVNGIPCHPSCQNCH*

• Vector Type: Mammalian
• Antibiotic: Amp
• Full Snapgene Map: pRetroX Tight FLAG-HOIL1461.66 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU65730
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee