SARS-CoV2 (2019-nCoV) Nsp2

• Plasmid: pGEX6P1 SARS-CoV2 (2019-nCoV) Nsp2
• Parent Plasmid: pGEX6P1
• Expressed: SARS-CoV2 (2019-nCoV) Nsp2
• Genbank: MN908947.3
• Tag: GST
• Species: SARS-CoV-2

Sequence of Insert

GGATCCGCGTATACCCGCTATGTGGATAACAACTTTTGCGGCCCGGATGGCTATCCGCTGGAATGCATTAAAGATCTGCTGGCGCGCGCGGGCAAAGCGAGCTGCACCCTGAGCGAACAGCTGGATTTTATTGATACCAAACGCGGCGTGTATTGCTGCCGCGAACATGAACATGAAATTGCGTGGTATACCGAACGCAGCGAAAAAAGCTATGAACTGCAGACCCCGTTTGAAATTAAACTGGCGAAAAAATTTGATACCTTTAACGGCGAATGCCCGAACTTTGTGTTTCCGCTGAACAGCATTATTAAAACCATTCAGCCGCGCGTGGAAAAAAAAAAACTGGATGGCTTTATGGGCCGCATTCGCAGCGTGTATCCGGTGGCGAGCCCGAACGAATGCAACCAGATGTGCCTGAGCACCCTGATGAAATGCGATCATTGCGGCGAAACCAGCTGGCAGACCGGCGATTTTGTGAAAGCGACCTGCGAATTTTGCGGCACCGAAAACCTGACCAAAGAAGGCGCGACCACCTGCGGCTATCTGCCGCAGAACGCGGTGGTGAAAATTTATTGCCCGGCGTGCCATAACAGCGAAGTGGGCCCGGAACATAGCCTGGCGGAATATCATAACGAAAGCGGCCTGAAAACCATTCTGCGCAAAGGtGGaCGCACCATTGCGTTTGGCGGCTGCGTGTTTAGCTATGTGGGCTGCCATAACAAATGCGCGTATTGGGTGCCGCGCGCGAGCGCGAACATTGGCTGCAACCATACCGGCGTGGTGGGCGAAGGCAGCGAAGGCCTGAACGATAACCTGCTGGAAATTCTGCAGAAAGAAAAAGTGAACATTAACATTGTGGGCGATTTTAAACTGAACGAAGAAATTGCGATTATTCTGGCGAGCTTTAGCGCGAGCACCAGCGCGTTTGTGGAAACCGTGAAAGGCCTGGATTATAAAGCGTTTAAACAGATTGTGGAAAGCTGCGGCAACTTTAAAGTGACCAAAGGCAAAGCGAAAAAAGGCGCGTGGAACATTGGCGAACAGAAAAGCATTCTGAGCCCGCTGTATGCGTTTGCGAGCGAAGCGGCGCGCGTGGTGCGCAGCATTTTTAGCCGCACCCTGGAAACCGCGCAGAACAGCGTGCGCGTGCTGCAGAAAGCGGCGATTACCATTCTGGATGGCATTAGCCAGTATAGCCTGCGCCTGATTGATGCGATGATGTTTACCAGCGATCTGGCGACCAACAACCTGGTGGTGATGGCGTATATTACCGGCGGCGTGGTGCAGCTGACCAGCCAGTGGCTGACCAACATTTTTGGCACCGTGTATGAAAAACTGAAACCGGTGCTGGATTGGCTGGAAGAAAAATTTAAAGAAGGCGTGGAATTTCTGCGCGATGGCTGGGAAATTGTGAAATTTATTAGCACCTGCGCGTGCGAAATTGTGGGCGGCCAGATTGTGACCTGCGCGAAAGAAATTAAAGAAAGCGTGCAGACCTTTTTTAAACTGGTGAACAAATTTCTGGCGCTGTGCGCGGATAGCATTATTATTGGCGGCGCGAAACTGAAAGCGCTGAACCTGGGCGAAACCTTTGTGACCCATAGCAAAGGCCTGTATCGCAAATGCGTGAAAAGCCGCGAAGAAACCGGCCTGCTGATGCCGCTGAAAGCGCCGAAAGAAATTATTTTTCTGGAAGGCGAAACCCTGCCGACCGAAGTGCTGACCGAAGAAGTGGTGCTGAAAACCGGCGATCTGCAGCCGCTGGAACAGCCGACCAGCGAAGCGGTGGAAGCGCCGCTGGTGGGCACCCCGGTGTGCATTAACGGCCTGATGCTGCTGGAAATTAAAGATACCGAAAAATATTGCGCGCTGGCGCCGAACATGATGGTGACCAACAACACCTTTACCCTGAAAGGCGGCtgaGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSAYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P1 SARS-CoV2 (2019-nCoV) Nsp2193.9 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU66414
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee