A20

• Plasmid: pRetrox tight FLAG-A20 A586V A588V
• Parent Plasmid: pRetrox tight FLAG
• Expressed: FLAG-A20 A586V A588V
• Genbank: NM_001270508.2 NP_001257437.1
• Tag: FLAG
• Species: Human

Sequence of Insert

ggatctATGGCTGAACAAGTCCTTCCTCAGGCTTTGTATTTGAGCAATAT
GCGGAAAGCTGTGAAGATACGGGAGAGAACTCCAGAAGACATTTTTAAAC
CTACTAATGGGATCATTCATCATTTTAAAACCATGCACCGATACACACTG
GAAATGTTCAGAACTTGCCAGTTTTGTCCTCAGTTTCGGGAGATCATCCA
CAAAGCCCTCATCGACAGAAACATCCAGGCCACCCTGGAAAGCCAGAAGA
AACTCAACTGGTGTCGAGAAGTCCGGAAGCTTGTGGCGCTGAAAACGAAC
GGTGACGGCAATTGCCTCATGCATGCCACTTCTCAGTACATGTGGGGCGT
TCAGGACACAGACTTGGTACTGAGGAAGGCGCTGTTCAGCACGCTCAAGG
AAACAGACACACGCAACTTTAAATTCCGCTGGCAACTGGAGTCTCTCAAA
TCTCAGGAATTTGTTGAAACGGGGCTTTGCTATGATACTCGGAACTGGAA
TGATGAATGGGACAATCTTATCAAAATGGCTTCCACAGACACACCCATGG
CCCGAAGTGGACTTCAGTACAACTCACTGGAAGAAATACACATATTTGTC
CTTTGCAACATCCTCAGAAGGCCAATCATTGTCATTTCAGACAAAATGCT
AAGAAGTTTGGAATCAGGTTCCAATTTCGCCCCTTTGAAAGTGGGTGGAA
TTTACTTGCCTCTCCACTGGCCTGCCCAGGAATGCTACAGATACCCCATT
GTTCTCGGCTATGACAGCCATCATTTTGTACCCTTGGTGACCCTGAAGGA
CAGTGGGCCTGAAATCCGAGCTGTTCCACTTGTTAACAGAGACCGGGGAA
GATTTGAAGACTTAAAAGTTCACTTTTTGACAGATCCTGAAAATGAGATG
AAGGAGAAGCTCTTAAAAGAGTACTTAATGGTGATAGAAATCCCCGTCCA
AGGCTGGGACCATGGCACAACTCATCTCATCAATGCCGCAAAGTTGGATG
AAGCTAACTTACCAAAAGAAATCAATCTGGTAGATGATTACTTTGAACTT
GTTCAGCATGAGTACAAGAAATGGCAGGAAAACAGCGAGCAGGGGAGGAG
AGAGGGGCACGCCCAGAATCCCATGGAACCTTCCGTGCCCCAGCTTTCTC
TCATGGATGTAAAATGTGAAACGCCCAACTGCCCCTTCTTCATGTCTGTG
AACACCCAGCCTTTATGCCATGAGTGCTCAGAGAGGCGGCAAAAGAATCA
AAACAAACTCCCAAAGCTGAACTCCAAGCCGGGCCCTGAGGGGCTCCCTG
GCATGGCGCTCGGGGCCTCTCGGGGAGAAGCCTATGAGCCCTTGGCGTGG
AACCCTGAGGAGTCCACTGGGGGGCCTCATTCGGCCCCACCGACAGCACC
CAGCCCTTTTCTGTTCAGTGAGACCACTGCCATGAAGTGCAGGAGCCCCG
GCTGCCCCTTCACACTGAATGTGCAGCACAACGGATTTTGTGAACGTTGC
CACAACGCCCGGCAACTTCACGCCAGCCACGCCCCAGACCACACAAGGCA
CTTGGATCCCGGGAAGTGCCAAGCCTGCCTCCAGGATGTTACCAGGACAT
TTAATGGGATCTGCAGTACTTGCTTCAAAAGGACTACAGCAGAGGCCTCC
TCCAGCCTCAGCACCAGCCTCCCTCCTTCCTGTCACCAGCGTTCCAAGTC
AGATCCCTCGCGGCTCGTCCGGAGCCCCTCCCCGCATTCTTGCCACAGAG
CTGGAAACGACGtCCCTGtTGGCTGCCTGTCTCAAGCTGCACGGACTCCT
GGGGACAGGACGGGGACGAGCAAGTGCAGAAAAGCCGGCTGCGTGTATTT
TGGGACTCCAGAAAACAAGGGCTTTTGCACACTGTGTTTCATCGAGTACA
GAGAAAACAAACATTTTGCTGCTGCCTCAGGGAAAGTCAGTCCCACAGCG
TCCAGGTTCCAGAACACCATTCCGTGCCTGGGGAGGGAATGCGGCACCCT
TGGAAGCACCATGTTTGAAGGATACTGCCAGAAGTGTTTCATTGAAGCTC
AGAATCAGAGATTTCATGAGGCCAAAAGGACAGAAGAGCAACTGAGATCG
AGCCAGCGCAGAGATGTGCCTCGAACCACACAAAGCACCTCAAGGCCCAA
GTGCGCCCGGGCCTCCTGCAAGAACATCCTGGCCTGCCGCAGCGAGGAGC
TCTGCATGGAGTGTCAGCATCCCAACCAGAGGATGGGCCCTGGGGCCCAC
CGGGGTGAGCCTGCCCCCGAAGACCCCCCCAAGCAGCGTTGCCGGGCCCC
CGCCTGTGATCATTTTGGCAATGCCAAGTGCAACGGCTACTGCAACGAAT
GCTTTCAGTTCAAGCAGATGTATGGCTAAgcggccgc

Amino Acid Sequence

MDYKDDDDKGSMAEQVLPQALYLSNMRKAVKIRERTPEDIFKPTNGIIHH
FKTMHRYTLEMFRTCQFCPQFREIIHKALIDRNIQATLESQKKLNWCREV
RKLVALKTNGDGNCLMHATSQYMWGVQDTDLVLRKALFSTLKETDTRNFK
FRWQLESLKSQEFVETGLCYDTRNWNDEWDNLIKMASTDTPMARSGLQYN
SLEEIHIFVLCNILRRPIIVISDKMLRSLESGSNFAPLKVGGIYLPLHWP
AQECYRYPIVLGYDSHHFVPLVTLKDSGPEIRAVPLVNRDRGRFEDLKVH
FLTDPENEMKEKLLKEYLMVIEIPVQGWDHGTTHLINAAKLDEANLPKEI
NLVDDYFELVQHEYKKWQENSEQGRREGHAQNPMEPSVPQLSLMDVKCET
PNCPFFMSVNTQPLCHECSERRQKNQNKLPKLNSKPGPEGLPGMALGASR
GEAYEPLAWNPEESTGGPHSAPPTAPSPFLFSETTAMKCRSPGCPFTLNV
QHNGFCERCHNARQLHASHAPDHTRHLDPGKCQACLQDVTRTFNGICSTC
FKRTTAEASSSLSTSLPPSCHQRSKSDPSRLVRSPSPHSCHRAGNDVPVG
CLSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFCTLCFIEYRENKHFAA
ASGKVSPTASRFQNTIPCLGRECGTLGSTMFEGYCQKCFIEAQNQRFHEA
KRTEEQLRSSQRRDVPRTTQSTSRPKCARASCKNILACRSEELCMECQHP
NQRMGPGAHRGEPAPEDPPKQRCRAPACDHFGNAKCNGYCNECFQFKQMY
G*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU65477
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee