Rnf12 (Mouse)

• Synonyms: AL022832; AW743871; Ha1r; Rlim
• Plasmid: pGEX6P1 mouse Rnf12 S212E S214E S227E S229E
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C mouse Rnf12 S212E S214E S227E S229E
• Genbank: NM_011276.3 GI:47078288
• Tag: GST
• Species: Mouse

Sequence of Insert

GGATCCATGGAGAACTCAGATTCTAACGATAAAGGAAGTGACCAGTCTGCAGCTCAGCGCAGAAGTCAAATGGATCGCTTGGATCGGGAAGAGGCTTTCTATCAATTTGTGAATAATCTCAGTGAAGAAGATTATAGACTTATGAGAGACAACAATTTGCTGGGCACCCCAGGTGAAAGTACTGAGGAAGAGTTGCTCAGAAGACTACAACAAATTAAAGAGGGTCCACCACCACAGAGCCCCGATGAAAATAGAGCTGGAGAGTCTTCAGATGATGTGACCAACAGTGATTCTATAATAGATTGGCTTAACTCAGTCAGACAGACTGGAAATACAACAAGAAGTGGTCAGAGAGGAAACCAGTCCTGGAGAGCAGTGAGCCGGACTAATCCAAACAGCGGTGATTTCAGATTCAGTTTAGAGATAAATGTTAACCGTAACAATGGAAGCCAAACTTCCGAGAATGAAAGTGAACCATCCACTAGGCGTCTTAGTGTAGAAAACATGGAGAGCAGCAGCCAAAGGCAAATGGAAAATTCTGCATCTGAATCAGCATCGGCCAGACCTTCTCGAGCGGAGCGCAATTCAGCTGAAGCAGTAACAGAAGTGCCGACCACCAGAGCTCAGAGGAGGGCAAGAGAGCGGGAACCAGAGCATCGGAGAACCAGAGCAAGAGCTGAAAGAGAGAGGGAACCTCTGCAGCCAACAAGTGAAATTCCACGAAGAGCTCCGACCTTGGAGCAGTCTTCGGAAAACGAGCCTGAAGGAAGTTCTAGAACCCGGCACCATGTGACCCTGAGACAGCAGATATCTGGACCGGAGTTGTTAGGTAGAGGTCTTTTTGCAGCTTCTGGATCAAGAAACCCCTCTCAGGGGACAAGCTCTTCAGACACAGGTAGCAATAGTGAATCCTCAGGATCTGGCCAAAGACCTCCAACCATAGTTCTTGATCTTCAAGTAAGAAGAGTTCGTCCTGGAGAATACCGGCAGAGAGATAGTATAGCTAGCAGAACTCGATCAAGATCTCAGGCTCCGAACAACACAGTTACTTATGAAAGTGAACGTGGAGGTTTTAGGCGTACCTTTTCCCGTTCTGAACGTGCAGGTGTGAGAACCTATGTGAGTACTATCAGGATTCCTATTCGCAGAATCTTAAATACTGGATTAAGTGAAACTACATCTGTTGCAATTCAAACCATGTTAAGGCAGATAATGACAGGCTTTGGTGAGCTAAGCTACTTTATGTACAGTGACAGTGATTCAGAGCCTAGTGCCTCAGTCTCCAGCCGAAATGTGGAGAGAGTAGAGTCACGGAATGGAAGAGGGAGCTCTGGTGGCGGTAACAGCTCTGGTTCCAGTTCCAGCTCAAGTCCTAGCCCCAGTTCAAGTGGTGAAAGTTCCGAGAGTAGCTCAGAGATGTTCGAAGGCAGTAGTGAAGGAGGCTCGTCTGGCCCATCCAGAAGAGATGGTCGACATAGGGCCCCAGTCACATTTGATGAAAGTGGCTCTCTGCCATTCCTTAGTCTGGCTCAGTTTTTTCTTTTAAATGAAGATGATGAAGACCAACCTAGAGGACTCACTAAAGAACAGATCGATAACTTGGCAATGAGAAGCTTTGGTGAAAATGATGCATTGAAAACGTGTAGTGTTTGCATTACAGAATACACAGAAGGCAACAAACTTCGTAAACTACCTTGCTCCCATGAGTACCACGTCCACTGCATCGACCGCTGGTTGTCTGAGAATTCCACCTGTCCCATTTGTCGCAGGGCAGTCTTATCTTCTGGGAACAGAGAAAGTGTTGTGTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMENSDSNDKGSDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQ
IKEGPPPQSPDENRAGESSDDVTNSDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQTSENESEPSTRRLSVENMES
SSQRQMENSASESASARPSRAERNSAEAVTEVPTTRAQRRAREREPEHRRTRARAEREREPLQPTSEIPRRAPTLEQSSENEPEGSSRTRHHVTLRQQIS
GPELLGRGLFAASGSRNPSQGTSSSDTGSNSESSGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQAPNNTVTYESERGGFRRTFSRSERAGVR
TYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPSASVSSRNVERVESRNGRGSSGGGNSSGSSSSSSPSPSSSGESSESSS
EMFEGSSEGGSSGPSRRDGRHRAPVTFDESGSLPFLSLAQFFLLNEDDEDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYH
VHCIDRWLSENSTCPICRRAVLSSGNRESVV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU58590
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee