USP1

• Synonyms: UBP
• Plasmid: pGEX6P1 codon optimised USP1 delta 1-15, 229-408 and 608-737 R35A
• Parent Plasmid: pGEX6P1
• Expressed: GST 3C codon optimised USP1 delta 1-15, 229-408 and 608-737 R35A
• Genbank: Codon optimised for expression in bacteria. Protein sequence matches that of NM_001017415.1 .
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCAGCCCGAGCAAAAAAAACCGTCTGAGCCTGAAATTTTTCCAGAAAAAAGAAACCAAAGCCGCCCTGGATTTTACCGATAGCCAAGAAAATGAAGAAAAAGCCAGTGAATATCGTGCCAGCGAAATTGATCAGGTTGTTCCGGCAGCACAGAGCAGCCCGATTAATTGTGAAAAACGTGAAAATCTGCTGCCGTTTGTGGGTCTGAATAATCTGGGTAATACCTGTTATCTGAACAGCATTCTGCAGGTCCTGTATTTTTGTCCGGGTTTTAAAAGCGGTGTGAAACACCTGTTTAACATTATCAGCCGTAAAAAAGAAGCCCTGAAAGACGAAGCAAATCAGAAAGATAAAGGCAACTGCAAAGAAGATAGCCTGGCAAGCTATGAACTGATTTGTAGCCTGCAGAGCCTGATTATTAGCGTTGAACAGCTGCAGGCAAGCTTTCTGCTGAATCCGGAAAAATACACCGATGAACTGGCAACCCAGCCTCGTCGCCTGCTGAATACCCTGCGTGAACTGAATCCGATGTATGAAGGTTATCTGCAGCATGATGCACAAGAGGTTCTGCAGTGTATTCTGGGCAATATTCAAGAAACCTGTCAGCTGCTGAAGAAAGAAGAGGTTAAAAACGTTGCCGAAAACGAAGTGAAACCGATTAACAAAGGCGAAGAACAAATTGGCTTTGAGCTGGTCGAAAAACTGTTCCAGGGCCAGCTGGTTCTGCGTACCCGTTGTCTGGAATGTGAAAGCCTGACCGAACGTCGTGAAGATTTTCAGGATATTAGCGTGCCGGTTCAAGAAGATGAACTGAGTAAAGTTGAAGAAAGCTCCGAAATTAGTCCGGAACCGAAAACCGAGATGAAAACCCTGCGCTGGGCAATTAGCCAGTTTGCAAGCGTGGAACGTATTGTTGGTGAAGATAAATACTTTTGCGAAAACTGCCACCATTATACCGAAGCAGAACGTAGCCTGCTGTTTGATAAAATGCCGGAAGTTATTACCATCCACCTGAAATGTTTTGCAGCAAGCGGTCTGGAATTTGATTGTTATGGTGGTGGTCTGTCCAAAATTAACACACCGCTGCTGACACCGCTGAAACTGAGCCTGGAAGAATGGTCAACCAAACCGACCAATGATAGCTATGGTCTGTTTGCCGTTGTTATGCATAGCGGTATTACCATTAGCAGCGGTCACTATACCGCAAGCGTTAAAGTTACCGATCTGAATTCACTGGAACAGAGTCTGAAAGAATATGAAGGTAAATGGCTGCTGTTCGATGATAGCGAAGTTAAAGTGACAGAAGAGAAAGACTTCCTGAATAGCCTGAGTCCGAGCACCAGTCCGACCAGCACACCGTATCTGCTGTTTTACAAAAAACTGTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSPSKKNRLSLKFFQKKETKAALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLN
NLGNTCYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNT
LRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAENEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDE
LSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSL
EEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLEQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU58465
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee