parkin

• Synonyms: PARK2, AR-JP, LPRS2, PDJ, PRKN, OTTHUMP00000017562, OTTHUMP00000017563, OTTHUMP00000017564, OTTHUMP00000017565, OTTHUMP00000017566, OTTHUMP00000017567, E3 ubiquitin ligase, E3 ubiquitin-protein ligase parkin, Parkinson disease (autosomal recessive, juvenile) 2, parkin, parkin 2, parkinson disease protein 2, parkinson juvenile disease protein 2
• Plasmid: pGEX6p-1-parkin S65A
• Parent Plasmid: pGEX6P-1
• Expressed: GST-parkin S65A
• Genbank: NM_004562.2
• Tag: GST
• Species: Human

Sequence of Insert

ggatccATGATAGTGTTTGTCAGGTTCAACTCCAGCCATGGTTTCCCAGTGGAGGT
CGATTCTGACACCAGCATCTTCCAGCTCAAGGAGGTGGTTGCTAAGCGAC
AGGGGGTTCCGGCTGACCAGTTGCGTGTGATTTTCGCAGGGAAGGAGCTG
AGGAATGACTGGACTGTGCAGAATTGTGACCTGGATCAGCAGgcCATTGT
TCACATTGTGCAGAGACCGTGGAGAAAAGGTCAAGAAATGAATGCAACTG
GAGGCGACGACCCCAGAAACGCGGCGGGAGGCTGTGAGCGGGAGCCCCAG
AGCTTGACTCGGGTGGACCTCAGCAGCTCAGTCCTCCCAGGAGACTCTGT
GGGGCTGGCTGTCATTCTGCACACTGACAGCAGGAAGGACTCACCACCAG
CTGGAAGTCCAGCAGGTAGATCAATCTACAACAGCTTTTATGTGTATTGC
AAAGGCCCCTGTCAAAGAGTGCAGCCGGGAAAACTCAGGGTACAGTGCAG
CACCTGCAGGCAGGCAACGCTCACCTTGACCCAGGGTCCATCTTGCTGGG
ATGATGTTTTAATTCCAAACCGGATGAGTGGTGAATGCCAATCCCCACAC
TGCCCTGGGACTAGTGCAGAATTTTTCTTTAAATGTGGAGCACACCCCAC
CTCTGACAAGGAAACATCAGTAGCTTTGCACCTGATCGCAACAAATAGTC
GGAACATCACTTGCATTACGTGCACAGACGTCAGGAGCCCCGTCCTGGTT
TTCCAGTGCAACTCCCGCCACGTGATTTGCTTAGACTGTTTCCACTTATA
CTGTGTGACAAGACTCAATGATCGGCAGTTTGTTCACGACCCTCAACTTG
GCTACTCCCTGCCTTGTGTGGCTGGCTGTCCCAACTCCTTGATTAAAGAG
CTCCATCACTTCAGGATTCTGGGAGAAGAGCAGTACAACCGGTACCAGCA
GTATGGTGCAGAGGAGTGTGTCCTGCAGATGGGGGGCGTGTTATGCCCCC
GCCCTGGCTGTGGAGCGGGGCTGCTGCCGGAGCCTGACCAGAGGAAAGTC
ACCTGCGAAGGGGGCAATGGCCTGGGCTGTGGGTTTGCCTTCTGCCGGGA
ATGTAAAGAAGCGTACCATGAAGGGGAGTGCAGTGCCGTATTTGAAGCCT
CAGGAACAACTACTCAGGCCTACAGAGTCGATGAAAGAGCCGCCGAGCAG
GCTCGTTGGGAAGCAGCCTCCAAAGAAACCATCAAGAAAACCACCAAGCC
CTGTCCCCGCTGCCATGTACCAGTGGAAAAAAATGGAGGCTGCATGCACA
TGAAGTGTCCGCAGCCCCAGTGCAGGCTCGAGTGGTGCTGGAACTGTGGC
TGCGAGTGGAACCGCGTCTGCATGGGGGACCACTGGTTCGACGTGTAGgcggccgc

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMIVFVRFNSSHGFPVEVDS
DTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQAIVHI
VQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGL
AVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTC
RQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSD
KETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCV
TRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYG
AEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECK
EAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCP
RCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6p-1-parkin S65A211.01 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU28775
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee