AMSH-like protease

• Plasmid: pcDNA FRT/TO BirA R118G AMSH LP HA
• Parent Plasmid: pcDNA FRT TO BirA-MCS-HA
• Expressed: BirA R118G-AMSH-HA
• Tag: HA
• Tag: BirA

Sequence of Insert

GCGGCCGCGATGGATCAGCCTTTTACTGTGAATTCTCTGAAAAAGTTAGCTGCTATGCCTGACCATACAGATGTTTCCCTAAGCCCAGAAGAGCGAGTCCGTGCCCTAAGCAAGCTTGGTTGTAATATCACCATCAGTGAAGACATCACTCCACGACGTTACTTTAGGTCTGGAGTAGAGATGGAGAGGATGGCGTCTGTGTATTTGGAAGAAGGAAATTTGGAAAATGCCTTTGTTCTTTATAATAAATTTATAACCTTATTTGTAGAAAAGCTTCCTAACCATCGAGATTACCAGCAATGTGCAGTACCTGAAAAGCAGGATATTATGAAGAAACTGAAGGAGATTGCATTCCCAAGGACAGATGAATTGAAAAACGACCTTTTAAAGAAATATAACGTAGAATACCAAGAATATTTGCAAAGCAAAAACAAATATAAAGCTGAAATTCTCAAAAAATTGGAGCATCAGAGATTGATAGAGGCAGAAAGGAAGCGGATTGCTCAGATGCGCCAGCAGCAGCTAGAATCGGAGCAGTTTCTGTTTTTCGAAGATCAACTCAAGAAGCAAGAGTTAGCCCGAGGTCAAATGCGAAGTCAGCAAACCTCAGGGCTGTCAGAGCAGATTGATGGGAGCGCTTTGTCCTGCTTTTCCACACACCAGAACAATTCCTTGCTGAATGTATTTGCAGATCAACCTAATAAAAGTGATGCAACCAATTATGCTAGCCACTCTCCTCCTGTAAACAGGGCCTTAACACCAGCTGCTACTCTAAGTGCTGTTCAGAATTTAGTGGTTGAAGGACTGCGATGTGTAGTTTTGCCAGAAGATCTTTGCCACAAATTTCTGCAACTGGCAGAATCTAATACAGTGAGAGGAATAGAAACCTGTGGAATACTCTGTGGAAAACTGACACATAATGAATTTACTATTACCCATGTAATTGTGCCAAAGCAGTCTGCGGGACCAGACTATTGTGACATGGAGAATGTAGAGGAATTATTCAATGTTCAGGATCAACATGATCTCCTCACTCTAGGATGGATCCATACACATCCCACTCAAACTGCATTTTTATCCAGCGTTGATCTTCACACTCACTGTTCCTATCAACTCATGTTGCCAGAGGCCATTGCCATTGTTTGCTCACCAAAGCATAAAGACACTGGCATCTTCAGGCTCACCAATGCTGGCATGCTTGAGGTTTCTGCTTGTAAAAAAAAGGGCTTTCATCCACACACCAAGGAGCCCAGGCTGTTCAGTATATGCAAACATGTGTTGGTAAAAGACATAAAAATAATTGTGTTGGATCTGAGGgcggccgc

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSPGEFASLEKLAAAMDQPFTVNSLKKLAAMPDHTDVSLSPEERVRALSKLGCNITISEDITPRRYFRSGVEMERMASVYLEEGNLENAFVLYNKFITLFVEKLPNHRDYQQCAVPEKQDIMKKLKEIAFPRTDELKNDLLKKYNVEYQEYLQSKNKYKAEILKKLEHQRLIEAERKRIAQMRQQQLESEQFLFFEDQLKKQELARGQMRSQQTSGLSEQIDGSALSCFSTHQNNSLLNVFADQPNKSDATNYASHSPPVNRALTPAATLSAVQNLVVEGLRCVVLPEDLCHKFLQLAESNTVRGIETCGILCGKLTHNEFTITHVIVPKQSAGPDYCDMENVEELFNVQDQHDLLTLGWIHTHPTQTAFLSSVDLHTHCSYQLMLPEAIAIVCSPKHKDTGIFRLTNAGMLEVSACKKKGFHPHTKEPRLFSICKHVLVKDIKIIVLDLRAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU55204
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee