USP1

• Plasmid: pcDNA FRT TO BirA R118G-USP1 (C90A)-HA
• Parent Plasmid: pcDNA BirAR118G-MCS-HA
• Expressed: BirA R118G-USP1 (C90A)-HA
• Genbank: NM_003368
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGCCTGGTGTCATACCTAGTGAAAGTAATGGACTTTCAAGAGGTAGCCCTTCAAAGAAAAACAGACTTTCCTTAAAGTTTTTTCAGAAAAAGGAAACTAAGAGAGCTTTGGATTTCACAGATTCTCAAGAAAATGAAGAAAAAGCTTCTGAATATAGAGCATCTGAAATTGATCAAGTTGTTCCTGCAGCACAGTCTTCACCTATAAACTGTGAGAAGAGAGAAAACTTGTTACCATTTGTGGGACTGAATAATCTCGGCAATACTGCCTATCTTAATAGTATACTTCAGGTATTATATTTTTGTCCCGGTTTTAAATCTGGAGTAAAGCACTTATTTAATATTATTTCAAGGAAGAAAGAAGCTCTAAAGGATGAAGCCAATCAAAAAGACAAGGGAAATTGCAAAGAAGATTCTTTGGCAAGTTATGAATTGATATGCAGTTTACAGTCCTTAATCATTTCGGTTGAACAGCTCCAGGCTAGTTTTCTCTTAAATCCAGAGAAATATACTGATGAACTTGCCACTCAGCCAAGGCGACTGCTTAACACACTGAGGGAACTCAACCCTATGTATGAAGGATATCTACAGCATGATGCACAGGAAGTATTACAATGTATTTTGGGAAACATTCAAGAAACATGCCAACTCCTAAAAAAAGAAGAAGTAAAAAATGTGGCAGAATTACCTACTAAGGTAGAAGAAATACCTCATCCGAAAGAGGAAATGAATGGTATTAACAGCATAGAGATGGACAGTATGAGGCATTCTGAAGACTTTAAAGAGAAACTCCCAAAAGGAAATGGGAAAAGAAAAAGTGACACTGAATTTGGTAACATGAAGAAAAAAGTTAAATTATCCAAGGAACACCAGTCATTGGAAGAGAACCAGAGACAAACTAGATCAAAAAGAAAAGCTACAAGTGATACATTAGAGAGTCCTCCTAAAATAATTCCCAAGTATATTTCTGAAAATGAGAGTCCAAGACCCTCACAAAAGAAATCAAGAGTTAAAATAAATTGGTTAAAGTCTGCAACTAAGCAACCCAGCATTCTTTCTAAATTTTGTAGTCTGGGAAAAATAACAACAAACCAAGGAGTCAAAGGACAATCTAAAGAAAATGAATGTGATCCTGAAGAGGACTTGGGGAAGTGTGAAAGTGATAACACAACTAATGGTTGTGGACTTGAATCTCCAGGAAATACTGTTACACCTGTAAATGTTAATGAAGTTAAACCCATAAACAAAGGTGAAGAACAAATTGGTTTTGAGCTAGTGGAGAAATTATTTCAAGGTCAGCTGGTATTAAGGACGCGTTGCTTGGAATGTGAAAGTTTAACAGAAAGAAGAGAAGATTTTCAAGACATCAGTGTGCCAGTACAAGAAGATGAGCTTTCCAAAGTAGAGGAGAGTTCTGAAATTTCTCCAGAGCCAAAAACAGAAATGAAGACCCTGAGATGGGCAATTTCACAATTTGCTTCAGTAGAAAGGATTGTAGGAGAAGATAAATATTTCTGTGAAAACTGCCATCATTATACTGAAGCTGAACGAAGTCTTTTGTTTGACAAAATGCCTGAAGTTATAACTATTCATTTGAAGTGCTTTGCTGCTAGTGGTTTGGAGTTTGATTGTTATGGTGGTGGACTTTCCAAGATCAACACTCCTTTATTGACACCTCTTAAATTGTCACTAGAAGAATGGAGCACAAAGCCAACTAACGACAGCTATGGATTATTTGCGGTTGTGATGCATAGTGGCATTACAATTAGTAGTGGGCATTACACTGCTTCTGTTAAAGTCACTGACCTTAACAGTTTAGAACTAGATAAAGGAAATTTTGTGGTTGACCAAATGTGTGAAATAGGTAAGCCAGAACCATTGAATGAGGAGGAAGCAAGGGGTGTGGTTGAGAATTATAATGATGAAGAAGTGTCAATTAGAGTTGGTGGAAATACACAGCCAAGTAAAGTTTTGAACAAAAAAAATGTAGAAGCTATTGGACTTCTTGGAGGACAAAAGAGCAAAGCAGATTATGAGCTATACAACAAAGCCTCTAATCCTGATAAGGTTGCTAGTACAGCGTTTGCTGAAAATAGAAATTCTGAGACTAGTGATACTACTGGGACCCATGAATCTGATAGAAACAAGGAATCCAGTGACCAAACAGGCATTAATATTAGTGGATTTGAGAACAAAATTTCATACGTAGTGCAAAGCTTAAAGGAGTATGAGGGGAAGTGGTTGCTTTTTGATGATTCTGAAGTCAAAGTTACTGAAGAGAAGGACTTTCTGAATTCTCTTTCCCCTTCTACATCTCCTACTTCTACTCCTTACTTGCTATTTTATAAGAAATTATAGGCGGCCGC

Amino Acid Sequence

MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGDACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEKGSMPGVIPSESNGLSRGSPSKKNRLSLKFFQKKETKRALDFTDSQENEEKASEYRASEIDQVVPAAQSSPINCEKRENLLPFVGLNNLGNTAYLNSILQVLYFCPGFKSGVKHLFNIISRKKEALKDEANQKDKGNCKEDSLASYELICSLQSLIISVEQLQASFLLNPEKYTDELATQPRRLLNTLRELNPMYEGYLQHDAQEVLQCILGNIQETCQLLKKEEVKNVAELPTKVEEIPHPKEEMNGINSIEMDSMRHSEDFKEKLPKGNGKRKSDTEFGNMKKKVKLSKEHQSLEENQRQTRSKRKATSDTLESPPKIIPKYISENESPRPSQKKSRVKINWLKSATKQPSILSKFCSLGKITTNQGVKGQSKENECDPEEDLGKCESDNTTNGCGLESPGNTVTPVNVNEVKPINKGEEQIGFELVEKLFQGQLVLRTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKLAAAYPYDVPDYA*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU47230
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee