USP35 Mammalian Optimised

• Plasmid: pcDNA5D FRT/TO USP35 (mammalian codon-opt)-GFP
• Parent Plasmid: pcDNA5D FRT/TO C-GFP
• Expressed: USP35 (codon-op)-GFP
• Genbank: Q9P2H5
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCGCCACCATGGACAAGATCCTGGAAGCCGTGGTCACCAGCAGCTACCCCGTGTCCGTGAAGCAGGGCCTCGTGCGGAGAGTGCTGGAAGCCGCCAGACAGCCCCTGGAACGCGAGCAGTGTCTGGCCCTGCTGGCTCTGGGCGCCAGACTGTATGTGGGCGGAGCCGAGGAACTGCCCAGAAGAGTGGGCTGCCAGCTGCTGCACGTGGCCGGCAGACACCACCCCGACGTGTTCGCCGAGTTCTTCAGCGCCAGACGGGTGCTGAGACTGCTGCAGGGCGGAGCTGGCCCTCCTGGACCTAGAGCCCTGGCCTGTGTGCAGCTGGGCCTGCAGCTGCTCCCTGAGGGACCTGCCGCCGATGAGGTGTTCGCCCTGCTGCGGCGGGAAGTGCTGAGAACCGTGTGCGAAAGACCCGGCCCTGCCGCCTGTGCTCAGGTGGCCAGACTGCTGGCCAGACACCCCAGATGCGTGCCCGACGGCCCCCACAGACTGCTGTTCTGTCAGCAGCTCGTGCGGTGCCTGGGCCGGTTCAGATGTCCTGCCGAAGGCGAAGAGGGCGCCGTGGAATTCCTGGAACAGGCCCAGCAGGTCAGCGGCCTGCTGGCACAGCTGTGGCGAGCCCAGCCTGCCGCCATCCTGCCCTGCCTGAAAGAACTGTTCGCCGTGATCAGCTGCGCCGAGGAAGAACCCCCTAGCAGCGCTCTGGCCAGCGTGGTGCAGCATCTGCCTCTGGAACTGATGGACGGCGTCGTGCGGAACCTGAGCAACGACGACAGCGTGACCGACAGCCAGATGCTGACCGCCATCAGCCGGATGATCGACTGGGTGTCCTGGCCCCTGGGCAAGAACATCGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCCGCCGTGAAGAAGTTCAGCATCCTGATCGAGGTGTCCCTGACCAAGATCGAGAAGGTGTTCAGCAAGCTGCTGTACCCCATCGTGCGGGGAGCCGCCCTGAGCGTGCTGAAGTACATGCTGCTGACCTTCCAGCACAGCCACGAGGCCTTCCATCTGCTGCTGCCCCACATCCCCCCCATGGTGGCCAGCCTGGTCAAAGAGGACAGCAACAGCGGCACCAGCTGCCTGGAACAGCTGGCCGAGCTGGTGCACTGCATGGTGTTCCGGTTCCCCGGCTTCCCCGACCTGTACGAGCCCGTGATGGAAGCCATCAAGGACCTGCACGTGCCCAACGAGGACCGGATCAAGCAGCTGCTGGGCCAGGACGCCTGGACCAGCCAGAAGTCCGAGCTGGCCGGCTTCTACCCCAGGCTGATGGCCAAGAGCGACACCGGCAAGATCGGCCTGATCAACCTGGGCAATACCTGCTACGTGAACAGCATCCTGCAGGCCCTGTTCATGGCCAGCGACTTCCGGCACTGCGTGCTGCGGCTGACCGAGAACAACAGCCAGCCCCTGATGACCAAGCTGCAGTGGCTGTTCGGCTTTCTGGAACACTCCCAGAGGCCCGCTATCAGCCCCGAGAACTTCCTGAGCGCCAGCTGGACCCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCAGCGAGTACCTGAAGTATCTGCTGGACCGGCTGCACGAGGAAGAGAAAACCGGCACCCGGATCTGCCAGAAGCTGAAGCAGAGCAGCAGCCCCAGCCCCCCTGAGGAACCTCCTGCCCCAAGCAGCACCAGCGTGGAAAAGATGTTCGGCGGAAAGATCGTGACCAGAATCTGCTGCCTGTGCTGTCTGAACGTGTCCAGCCGGGAAGAGGCCTTCACCGACCTGAGCCTGGCCTTCCCACCCCCCGAGAGATGCAGAAGGCGGAGACTGGGCAGCGTGATGCGGCCCACCGAGGACATCACCGCCAGAGAGCTGCCTCCCCCAACAAGCGCCCAGGGACCTGGCAGAGTGGGCCCCAGACGGCAGCGGAAGCACTGCATCACCGAGGATACCCCCCCCACCAGCCTGTACATCGAGGGCCTGGACAGCAAAGAGGCTGGCGGCCAGAGCAGCCAGGAAGAGAGAATCGAGCGCGAGGAAGAGGGCAAAGAGGAACGCACCGAGAAAGAGGAAGTCGGCGAGGAAGAAGAGAGCACCAGAGGCGAGGGCGAGCGCGAAAAAGAGGAAGAAGTCGAAGAGGAAGAGGAAAAGGTCGAGAAAGAGACAGAGAAAGAGGCCGAGCAGGAAAAAGAAGAGGACTCCCTGGGAGCCGGCACCCACCCCGATGCCGCCATTCCTAGCGGCGAGAGAACCTGCGGCAGCGAGGGCAGCAGAAGCGTGCTGGACCTGGTCAACTACTTCCTGTCCCCCGAGAAGCTGACCGCCGAGAACCGGTACTACTGCGAGAGCTGCGCCAGCCTGCAGGACGCCGAGAAGGTGGTGGAACTGAGCCAGGGCCCCTGCTACCTGATCCTGACCCTGCTGAGATTCAGCTTCGACCTGCGGACCATGCGGCGGAGAAAGATTCTGGACGACGTGTCCATCCCCCTGCTGCTGAGGCTGCCTCTGGCTGGCGGAAGAGGCCAGGCCTACGACCTGTGCAGCGTGGTGGTGCACAGCGGCGTGTCCAGCGAGAGCGGCCACTACTACTGCTACGCCAGAGAGGGCGCTGCCAGACCAGCCGCCTCTCTGGGCACAGCCGATAGACCCGAGCCCGAGAACCAGTGGTATCTGTTCAACGACACCAGAGTGTCCTTCAGCAGCTTCGAGAGCGTGTCCAACGTGACCTCATTCTTCCCAAAGGACACCGCCTACGTGCTGTTCTACCGGCAGCGGCCTAGAGAGGGCCCTGAGGCCGAACTGGGCAGCTCCAGAGTGCGGACCGAGCCCACCCTGCACAAGGACCTGATGGAAGCTATCTCCAAGGACAACATCCTGTATCTGCAGGAACAGGAAAAAGAGGCCAGAAGCAGAGCCGCCTACATCAGCGCCCTGCCCACCAGCCCTCACTGGGGCAGAGGCTTCGACGAGGACAAGGATGAGGACGAGGGCAGCCCTGGCGGCTGCAACCCTGCTGGCGGCAATGGCGGCGACTTCCACCGGCTGGTGTTCGCGGCCGC

Amino Acid Sequence

MDKILEAVVTSSYPVSVKQGLVRRVLEAARQPLEREQCLALLALGARLYV
GGAEELPRRVGCQLLHVAGRHHPDVFAEFFSARRVLRLLQGGAGPPGPRA
LACVQLGLQLLPEGPAADEVFALLRREVLRTVCERPGPAACAQVARLLAR
HPRCVPDGPHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLA
QLWRAQPAAILPCLKELFAVISCAEEEPPSSALASVVQHLPLELMDGVVR
NLSNDDSVTDSQMLTAISRMIDWVSWPLGKNIDKWIIALLKGLAAVKKFS
ILIEVSLTKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPH
IPPMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKD
LHVPNEDRIKQLLGQDAWTSQKSELAGFYPRLMAKSDTGKIGLINLGNTC
YVNSILQALFMASDFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAIS
PENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSS
SPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAF
PPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITE
DTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEEST
RGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGE
RTCGSEGSRSVLDLVNYFLSPEKLTAENRYYCESCASLQDAEKVVELSQG
PCYLILTLLRFSFDLRTMRRRKILDDVSIPLLLRLPLAGGRGQAYDLCSV
VVHSGVSSESGHYYCYAREGAARPAASLGTADRPEPENQWYLFNDTRVSF
SSFESVSNVTSFFPKDTAYVLFYRQRPREGPEAELGSSRVRTEPTLHKDL
MEAISKDNILYLQEQEKEARSRAAYISALPTSPHWGRGFDEDKDEDEGSP
GGCNPAGGNGGDFHRLVFAAAMVSKGEELFTGVVPILVELDGDVNGHKFS
VSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM
KQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGI
DFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQL
ADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAG
ITLGMDELYKSGL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU49581
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee