Parkin

• Plasmid: pET6HIS SUMO PArkin K161A
• Parent Plasmid: pET15b 6His SUMO1
• Expressed: His-SUMO Parkin [K161A]
• Genbank: O60260
• Tag: SUMO
• Tag: 6His
• Species: Human

Sequence of Insert

ATGGGTCATCATCACCATCACCATTCTGACCAGGAGGCAAAACCTTCAAC
TGAGGACTTGGGGGATAAGAAGGAAGGTGAATATATTAAACTCAAAGTCA
TTGGACAGGATAGCAGTGAGATTCACTTCAAAGTGAAAATGACAACACAT
CTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTGTTCCAATGAA
CTCACTCAGGTTTCTCTTTGAGGGTCAGAGAATTGCTGATAATCATACTC
CAAAAGAACTGGGAATGGAGGAAGAAGATGTGATTGAAGTTTATCAGGAA
CAAACGGGGGGAatgatagtgtttgtcaggttcaactccagccatggttt
cccagtggaggtcgattctgacaccagcatcttccagctcaaggaggtgg
ttgctaagcgacagggggttccggctgaccagttgcgtgtgattttcgca
gggaaggagctgaggaatgactggactgtgcagaattgtgacctggatca
gcagagcattgttcacattgtgcagagaccgtggagaaaaggtcaagaaa
tgaatgcaactggaggcgacgaccccagaaacgcggcgggaggctgtgag
cgggagccccagagcttgactcgggtggacctcagcagctcagtcctccc
aggagactctgtggggctggctgtcattctgcacactgacagcaggaagg
actcaccaccagctggaagtccagcaggtagatcaatctacaacagcttt
tatgtgtattgcaaaggcccctgtcaaagagtgcagccgggagcactcag
ggtacagtgcagcacctgcaggcaggcaacgctcaccttgacccagggtc
catcttgctgggatgatgttttaattccaaaccggatgagtggtgaatgc
caatccccacactgccctgggactagtgcagaatttttctttaaatgtgg
agcacaccccacctctgacaaggaaacatcagtagctttgcacctgatcg
caacaaatagtcggaacatcacttgcattacgtgcacagacgtcaggagc
cccgtcctggttttccagtgcaactcccgccacgtgatttgcttagactg
tttccacttatactgtgtgacaagactcaatgatcggcagtttgttcacg
accctcaacttggctactccctgccttgtgtggctggctgtcccaactcc
ttgattaaagagctccatcacttcaggattctgggagaagagcagtacaa
ccggtaccagcagtatggtgcagaggagtgtgtcctgcagatggggggcg
tgttatgcccccgccctggctgtggagcggggctgctgccggagcctgac
cagaggaaagtcacctgcgaagggggcaatggcctgggctgtgggtttgc
cttctgccgggaatgtaaagaagcgtaccatgaaggggagtgcagtgccg
tatttgaagcctcaggaacaactactcaggcctacagagtcgatgaaaga
gccgccgagcaggctcgttgggaagcagcctccaaagaaaccatcaagaa
aaccaccaagccctgtccccgctgccatgtaccagtggaaaaaaatggag
gctgcatgcacatgaagtgtccgcagccccagtgcaggctcgagtggtgc
tggaactgtggctgcgagtggaaccgcgtctgcatgggggaccactggtt
cgacgtgtaggcggccgc

Amino Acid Sequence

MGHHHHHHSDQEAKPSTEDLGDKKEGEYIKLKVIGQDSSEIHFKVKMTTH
LKKLKESYCQRQGVPMNSLRFLFEGQRIADNHTPKELGMEEEDVIEVYQE
QTGGMIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFA
GKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCE
REPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSF
YVYCKGPCQRVQPGALRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGEC
QSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRS
PVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNS
LIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPD
QRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDER
AAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWC
WNCGCEWNRVCMGDHWFDV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU43800
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee