USP35

• Plasmid: pet28a DAC TEV USP35
• Parent Plasmid: pET28a DAC TEV
• Expressed: Dac-TEV-USP35
• Genbank: Q9P2H5
• Tag: DAC
• Species: Human

Sequence of Insert

GGATCCATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGT CAGCGTGAAGCAGGGGCTGGTTCGGCGCGTGCTGGAGGCGGCGCGGCAGC CGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCGCGCCTC TACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCT GCACGTGGCCGGCCGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCG CGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGCGCCGGCCCCCCGGGCCCC CGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCC TGCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCG TGTGCGAGCGCCCGGGCCCCGCGGCCTGCGCGCAGGTGGCACGGCTGCTG GCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGCCTGCTCTTCTG CCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCG AGGAGGGCGCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTC CTGGCGCAGCTGTGGCGCGCACAGCCCGCCGCCATCCTGCCCTGCCTCAA AGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCTAGCG CCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTT GTCCGGAACCTCAGCAATGATGACAGTGTGACAGACTCGCAGATGCTGAC TGCCATTAGCAGGATGATTGACTGGGTGTCCTGGCCCCTGGGGAAGAATA TTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAG TTCAGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTC TAAGCTGCTGTACCCCATCGTCCGGGGAGCTGCCTTGTCTGTGCTCAAGT ACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCACCTGCTCCTC CCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTC GGGGACCAGCTGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGT TCCGGTTCCCGGGCTTCCCGGATCTGTATGAGCCTGTCATGGAGGCCATC AAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGGCA GGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGC TCATGGCCAAGTCAGACACGGGCAAGATTGGTCTCATCAACCTGGGCAAC ACATGCTATGTCAACAGCATCCTTCAGGCCTTATTCATGGCGTCTGACTT CAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGA CCAAGCTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCC ATTTCCCCAGAGAACTTCCTCTCCGCATCCTGGACGCCCTGGTTCAGCCC TGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTGGATCGGC TGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAG TCCAGCTCGCCCTCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTC TGTGGAAAAAATGTTTGGAGGCAAGATAGTGACTCGGATCTGCTGTCTCT GCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGAT GCGCCCCACAGAAGACATCACAGCCCGGGAGTTGCCCCCACCAACCAGTG CACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGGAAACACTGCATC ACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAA GGAAGCTGGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAG AAGGGAAGGAGGAGAGAACGGAGAAGGAAGAAGTGGGGGAGGAGGAGGAA AGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAAGAGGA AGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGG AAGAAGACAGCCTGGGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCC GGGGAGCGGACATGTGGCTCTGAGGGCTCCCGCTCCGTCCTGGACCTGGT TAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACT GCGAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGC CAAGGGCCGTGCTACCTCATCCTCACACTGCTGCGCTTCTCTTTCGACCT GCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCCATCCCCCTGC TGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGC AGTGTGGTGGTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTG CTATGCCCGTGAGGGCGCTGCCCGCCCTGCCGCTTCTCTGGGAACTGCCG ATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGGGTG TCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAA GGACACAGCCTATGTGCTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCG AGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAGCCCACCCTGCACAAG GACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCA GGAGAAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACAT CTCCGCACTGGGGGAGGGGCTTTGATGAAGACAAGGATGAGGATGAAGGC TCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGACTTCCACAG ACTGGTCTTCTAAGCGGCCGC

Amino Acid Sequence

MSAIPGVPQIDAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIG
QAMKAGKFKETDLVTIGNDAWATGNPVFKGSSLMFLKPGMQVPVSQLIRG
INLQSGNDACVAMADFAAGSQDAFVGLMNSYVNALGLKNTHFQTVHGLDA
DGQYSSARDMALIGQALIRDVPNEYSIYKEKEFTFNGIRQLNRNGLLWDN
SLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKGREAESKKL
LTWGFRFFETVNPENLYFQGGSMDKILEAVVTSSYPVSVKQGLVRRVLEA
ARQPLEREQCLALLALGARLYVGGAEELPRRVGCQLLHVAGRHHPDVFAE
FFSARRVLRLLQGGAGPPGPRALACVQLGLQLLPEGPAADEVFALLRREV
LRTVCERPGPAACAQVARLLARHPRCVPDGPHRLLFCQQLVRCLGRFRCP
AEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAILPCLKELFAVISCAEEEP
PSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPL
GKNIDKWIIALLKGLAAVKKFSILIEVSLTKIEKVFSKLLYPIVRGAALS
VLKYMLLTFQHSHEAFHLLLPHIPPMVASLVKEDSNSGTSCLEQLAELVH
CMVFRFPGFPDLYEPVMEAIKDLHVPNEDRIKQLLGQDAWTSQKSELAGF
YPRLMAKSDTGKIGLINLGNTCYVNSILQALFMASDFRHCVLRLTENNSQ
PLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYL
LDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRI
CCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPP
PTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIE
REEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAE
QEKEEDSLGAGTHPDAAIPSGERTCGSEGSRSVLDLVNYFLSPEKLTAEN
RYYCESCASLQDAEKVVELSQGPCYLILTLLRFSFDLRTMRRRKILDDVS
IPLLLRLPLAGGRGQAYDLCSVVVHSGVSSESGHYYCYAREGAARPAASL
GTADRPEPENQWYLFNDTRVSFSSFESVSNVTSFFPKDTAYVLFYRQRPR
EGPEAELGSSRVRTEPTLHKDLMEAISKDNILYLQEQEKEARSRAAYISA
LPTSPHWGRGFDEDKDEDEGSPGGCNPAGGNGGDFHRLVF*

• Vector Type: Bacterial
• Antibiotic: Kan
• DU Number: DU46087
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee