Lysates of RAW264.7 macrophages (1 mg cell extract) were incubated with 5 ug of antibody from non-immunised sheep (IgG) or antibodies against NEMO. The immunoprecipitates were washed, separated by SDS-Page and immunoblotted with the antibodies indicated. Please note that NEMO is a component of the canonical IKK complex and that other components of the complex (the catalytic subunits IKK alpha and IKK beta) are also immunoprecipitated with the NEMO antibody.