Human Recombinant Protein - PKN1 (460- 942)

Name: PKN1 (460- 942)
Price per aliquot (100µg): £110.00
Long Name:
protein kinase N1
Synonyms:
PRKCL1, protein kinase C-like 1, DBK , PRK1, PKN, MGC46204 , PAK1
Species:
Human
Catalog Number (DU Number):
DU68070
Accession:
Q16512-2
Expression:
baculovirus
Tag and Terminus:
N-Term
GST
Uncleaved
Purification Method:
GSH-Agarose
Enzymatic Buffer:
50 mM Tris-HCl pH 7.5, 0.1mM EGTA, 10 mM DTT, 10 mM MgAc
Enzymatic Substrate:
KKLNRTLSVA
Calculated molecular mass:
Mono-Isotopic Mass: 81, 376.31 daltons
Average Mass: 81, 428.17 daltons
Protein Activity:
Constitutively Active
Purity:
>75 %
Storage Buffer:
50 mM Tris-HCl pH 7.5, 270 mM Sucrose, 150 mM NaCl, 0.1 mM EGTA, 0.1 % 2-mercaptoethanol, 0.02 % Brij-35, 1 mM benzamidine, 0.2 mM PMSF
Storage Temperature:
-70 deg C
Theoretical PI:
5.40
Native Sequence:
Amino acids D460 – D942 (end residue C948) of human PKN1. Residue D239 of the fusion protein is equivalent to M1 of the native enzyme. The GST tag is located at residues 1 – 220.
Protease Cleavage:
PreScission (LEVLFQGP) residues 221 - 228
Cloning Sites:
Xho1 - Not1 into Sal1 - Not1 sites of pFastBac GST

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