cDNA Clone LONRF2

• Synonyms: .
• Plasmid: pBABED P FLAG c-5GLY-aGFP16-LONRF2 His339-Asn754 (end)
• Parent Plasmid: pBABED P FLAG C-5GLY-aGFP16
• Expressed: FLAG c-5GLY-aGFP16-LONRF2 His339-Asn754
• Genbank: GeneID 164832
• Species: Human

Sequence of Insert

gtcgacCACATGAATGCCCAGGCTCTGCTGGAAGAAGGTGATGCAGGGAG
CTCGGAGAATTCATCTGAGAAATCTGATATGCTTGGAAATACCAATTCTT
CAGTTTTGTATTTTATACTGGGTCTACACTTCGAAGAGGATAAAAAGGCG
TTAGAAAGCATCCTTCCAACAGCACCCAGCGCTGGCTTAAAGAGACAGTT
TCCGGATGACGTGGAGGATGCACCTGACCTGAACGCCCCTGGAAAAATTC
CCAAGAAAGATCTCTCACCTCAAAGGAGCCCAAACTCTGAGACAGAAGAA
AGTCAGGGGCTCTCGCTTGATGTAACTGACTTTGAGTGTGCCCTCTGCAT
GAGATTGCTCTTTGAACCTGTCACTACGCCCTGTGGACACACATTTTGCC
TAAAATGCCTTGAGCGCTGCCTTGACCACGCCCCACACTGTCCTTTGTGC
AAAGACAAACTTTCGGAATTATTGGCAAGCAGAAACTTTAACATAACTGT
TCTGGCCGAAGAATTAATATTTCGATATTTGCCGGATGAATTGTCTGATA
GGAAGAGAATTTATGATGAAGAAATGTCAGAACTGTCAAATCTGACCAGA
GACGTCCCCATCTTTGTGTGTGCCATGGCCTTCCCCACGGTCCCATGTCC
ACTCCACGTTTTTGAGCCCCGCTATCGGCTTATGATAAGAAGATGCATGG
AAACTGGCACCAAGCGGTTTGGCATGTGTTTATCTGCTGAGCACGCGGGG
CTTTCAGAGTATGGATGCATGCTGGAGATTAAGGACGTGAGAACGTTTCC
TGATGGAAGTTCTGTTGTAGACGCGATTGGCATCAGTCGGTTCCGAGTGC
TAAGCCACCGCCACAGAGATGGCTATAACACAGCGGACATTGAATATCTT
GAAGATGAAAAGGTGGAGGGTCCAGAGTATGAAGAACTTGCCGCTCTCCA
CGATTCTGTGCATCAACAGTCTGTTTCCTGGTTCGCGTCTCTCCAGGATC
GCATGAAAGAACAAATTTTAAGTCATTTTGGGGTAATGCCAGACAGAGAA
CCTGAGCCTCAGAGTAATCCCAGCGGCCCTGCCTGGTCCTGGTGGATCCT
GGCCGTGCTGCCCCTGGAGCGCAAGGCTCAGCTGGCCATCCTCGGCATGA
CCTCGCTCAAAGAGCGGCTCCTTGCCATCCGACGGATATTAGTCATCATC
ACGCGTAAGATGAATAGTCGGCAAGAGCTGGCTAATGCCAGGGAGAGAAA
TAATgcggccgc

Amino Acid Sequence

MDYKDDDDKGSSEFEVDHMNAQALLEEGDAGSSENSSEKSDMLGNTNSSV
LYFILGLHFEEDKKALESILPTAPSAGLKRQFPDDVEDAPDLNAPGKIPK
KDLSPQRSPNSETEESQGLSLDVTDFECALCMRLLFEPVTTPCGHTFCLK
CLERCLDHAPHCPLCKDKLSELLASRNFNITVLAEELIFRYLPDELSDRK
RIYDEEMSELSNLTRDVPIFVCAMAFPTVPCPLHVFEPRYRLMIRRCMET
GTKRFGMCLSAEHAGLSEYGCMLEIKDVRTFPDGSSVVDAIGISRFRVLS
HRHRDGYNTADIEYLEDEKVEGPEYEELAALHDSVHQQSVSWFASLQDRM
KEQILSHFGVMPDREPEPQSNPSGPAWSWWILAVLPLERKAQLAILGMTS
LKERLLAIRRILVIITRKMNSRQELANARERNNAAAGGGGGMAQVQLVES
GGRLVQAGDSLRLSCAASGRTFSTSAMAWFRQAPGREREFVAAITWTVGN
TILGDSVKGRFTISRDRAKNTVDLQMDNLEPEDTAVYYCSARSRGYVLSV
LRSVDSYDYWGQGTQVTVSG*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29618
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 60052; Silent change at Phe380 (TTT > TTC) plus listed SNP Leu426Pro
• Low Temp: No