cDNA Clone LONRF2

• Synonyms: .
• Plasmid: pBABED P FLAG aGFP16 5GLY- LONRF2 His339-Asn754 (end)
• Parent Plasmid: pBABED P FLAG aGFP16 5GLY
• Expressed: FLAG aGFP16 5GLY- LONRF2 His339-Asn754
• Genbank: GeneID 164832
• Tag: FLAG
• Species: Human

Sequence of Insert

gtcgacCACATGAATGCCCAGGCTCTGCTGGAAGAAGGTGATGCAGGGAG
TTCGGAGAATTCATCTGAGAAATCTGATATGCTTGGAAATACCAATTCTT
CAGTTTTGTATTTTATACTGGGTCTACACTTCGAAGAGGATAAAAAGGCG
TTAGAAAGCATCCTTCCAACAGCACCCAGCGCTGGCTTAAAGAGACAGTT
TCCGGATGACGTGGAGGATGCACCTGACCTGAACGCCCCTGGAAAAATTC
CCAAGAAAGATCTCTCACCTCAAAGGAGCCCAAACTCTGAGACAGAAGAA
AGTCAGGGGCTCTCGCTTGATGTAACTGACTTTGAGTGTGCCCTCTGCAT
GAGATTGCTCTTTGAACCTGTCACTACGCCCTGTGGACACACATTTTGCC
TAAAATGCCTTGAGCGCTGCCTTGACCACGCCCCACACTGTCCTTTGTGC
AAAGACAAACTTTCGGAATTATTGGCAAGCAGAAACTTTAACATAACTGT
TCTGGCCGAAGAATTAATATTTCGATATTTGCCGGATGAATTGTCTGATA
GGAAGAGAATTTATGATGAAGAAATGTCAGAACTGTCAAATCTGACCAGA
GACGTCCCCATCTTTGTGTGTGCCATGGCCTTCCCCACGGTCCCATGTCC
ACTCCACGTTTTTGAGCCCCGCTATCGGCTTATGATAAGAAGATGCATGG
AAACTGGCACCAAGCGGTTTGGCATGTGTTTATCTGCTGAGCACGCGGGG
CTTTCAGAGTATGGATGCATGCTGGAGATTAAGGACGTGAGAACGTTTCC
TGATGGAAGTTCTGTTGTAGACGCGATTGGCATCAGTCGGTTCCGAGTGC
TAAGCCACCGCCACAGAGATGGCTATAACACAGCGGACATTGAATATCTT
GAAGATGAAAAGGTGGAGGGTCCAGAGTATGAAGAACTTGCCGCTCTCCA
CGATTCTGTGCATCAACAGTCTGTTTCCTGGTTCGCGTCTCTCCAGGATC
GCATGAAAGAACAAATTTTAAGTCATTTTGGGGTAATGCCAGACAGAGAA
CCTGAGCCTCAGAGTAATCCCAGCGGCCCTGCCTGGTCCTGGTGGATCCT
GGCCGTGCTGCCCCTGGAGCGCAAGGCTCAGCTGGCCATCCTCGGCATGA
CCTCGCTCAAAGAGCGGCTCCTTGCCATCCGACGGATATTAGTCATCATC
ACGCGTAAGATGAATAGTCGGCAAGAGCTGGCTAATGCCAGGGAGAGAAA
TAATtgagcggccgc

Amino Acid Sequence

MDYKDDDDKGSMAQVQLVESGGRLVQAGDSLRLSCAASGRTFSTSAMAWF
RQAPGREREFVAAITWTVGNTILGDSVKGRFTISRDRAKNTVDLQMDNLE
PEDTAVYYCSARSRGYVLSVLRSVDSYDYWGQGTQVTVSGGGGGGGSSEF
EVDHMNAQALLEEGDAGSSENSSEKSDMLGNTNSSVLYFILGLHFEEDKK
ALESILPTAPSAGLKRQFPDDVEDAPDLNAPGKIPKKDLSPQRSPNSETE
ESQGLSLDVTDFECALCMRLLFEPVTTPCGHTFCLKCLERCLDHAPHCPL
CKDKLSELLASRNFNITVLAEELIFRYLPDELSDRKRIYDEEMSELSNLT
RDVPIFVCAMAFPTVPCPLHVFEPRYRLMIRRCMETGTKRFGMCLSAEHA
GLSEYGCMLEIKDVRTFPDGSSVVDAIGISRFRVLSHRHRDGYNTADIEY
LEDEKVEGPEYEELAALHDSVHQQSVSWFASLQDRMKEQILSHFGVMPDR
EPEPQSNPSGPAWSWWILAVLPLERKAQLAILGMTSLKERLLAIRRILVI
ITRKMNSRQELANARERNN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU29617
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Parental 60093; Silent change at Phe380 (TTT > TTC) plus listed SNP Leu426Pro
• Low Temp: No